Experiment: LB in EcoFAB_10mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14000 and BWI76_RS14005 are separated by 2 nucleotides BWI76_RS14005 and BWI76_RS14010 are separated by 116 nucleotides BWI76_RS14010 and BWI76_RS14015 are separated by 265 nucleotides
BWI76_RS14000: BWI76_RS14000 - glutamine amidotransferase, at 2,925,897 to 2,926,640
_RS14000
BWI76_RS14005: BWI76_RS14005 - TetR family transcriptional regulator, at 2,926,643 to 2,927,191
_RS14005
BWI76_RS14010: BWI76_RS14010 - SDR family oxidoreductase, at 2,927,308 to 2,928,090
_RS14010
BWI76_RS14015: BWI76_RS14015 - L-2,4-diaminobutyrate decarboxylase, at 2,928,356 to 2,929,828
_RS14015
Position (kb)
2927
2928
2929 Strain fitness (log2 ratio)
-1
0
1
2 at 2926.339 kb on - strand, within BWI76_RS14000 at 2926.343 kb on + strand, within BWI76_RS14000 at 2926.523 kb on - strand, within BWI76_RS14000 at 2926.570 kb on + strand at 2926.816 kb on + strand, within BWI76_RS14005 at 2927.693 kb on + strand, within BWI76_RS14010 at 2927.773 kb on + strand, within BWI76_RS14010 at 2927.810 kb on + strand, within BWI76_RS14010 at 2927.957 kb on + strand, within BWI76_RS14010 at 2928.097 kb on + strand at 2928.114 kb on + strand at 2929.084 kb on + strand, within BWI76_RS14015
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_10mL remove 2,926,339 - BWI76_RS14000 0.59 +0.6 2,926,343 + BWI76_RS14000 0.60 -1.4 2,926,523 - BWI76_RS14000 0.84 -0.9 2,926,570 + -1.2 2,926,816 + BWI76_RS14005 0.32 -0.6 2,927,693 + BWI76_RS14010 0.49 +0.2 2,927,773 + BWI76_RS14010 0.59 -0.3 2,927,810 + BWI76_RS14010 0.64 +2.5 2,927,957 + BWI76_RS14010 0.83 -0.0 2,928,097 + -0.5 2,928,114 + +1.6 2,929,084 + BWI76_RS14015 0.49 -0.2
Or see this region's nucleotide sequence