Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhT and acpT are separated by 54 nucleotides acpT and nikA are separated by 110 nucleotides nikA and nikB overlap by 1 nucleotides nikB and nikC overlap by 4 nucleotides
b3474: yhhT - orf, hypothetical protein (VIMSS), at 3,609,888 to 3,610,937
yhhT
b3475: acpT - holo-(acyl carrier protein) synthase 2 (NCBI), at 3,610,992 to 3,611,579
acpT
b3476: nikA - nickel transporter subunit (NCBI), at 3,611,690 to 3,613,264
nikA
b3477: nikB - nickel transporter subunit (NCBI), at 3,613,264 to 3,614,208
nikB
b3478: nikC - nickel transporter subunit (NCBI), at 3,614,205 to 3,615,038
nikC
Position (kb)
3611
3612
3613
3614 Strain fitness (log2 ratio)
-2
-1
0
1 at 3610.757 kb on + strand, within yhhT at 3610.757 kb on + strand, within yhhT at 3610.760 kb on - strand, within yhhT at 3610.762 kb on + strand, within yhhT at 3610.837 kb on + strand at 3610.837 kb on + strand at 3610.944 kb on + strand at 3610.946 kb on + strand at 3610.989 kb on + strand at 3610.992 kb on - strand at 3611.002 kb on - strand at 3611.002 kb on - strand at 3611.031 kb on - strand at 3611.059 kb on + strand, within acpT at 3611.104 kb on + strand, within acpT at 3611.104 kb on + strand, within acpT at 3611.122 kb on + strand, within acpT at 3611.140 kb on + strand, within acpT at 3611.149 kb on + strand, within acpT at 3611.149 kb on + strand, within acpT at 3611.200 kb on + strand, within acpT at 3611.214 kb on + strand, within acpT at 3611.234 kb on + strand, within acpT at 3611.234 kb on + strand, within acpT at 3611.242 kb on - strand, within acpT at 3611.242 kb on - strand, within acpT at 3611.320 kb on + strand, within acpT at 3611.407 kb on + strand, within acpT at 3611.407 kb on + strand, within acpT at 3611.409 kb on + strand, within acpT at 3611.447 kb on - strand, within acpT at 3611.447 kb on - strand, within acpT at 3611.455 kb on + strand, within acpT at 3611.529 kb on - strand at 3611.530 kb on + strand at 3611.530 kb on + strand at 3611.538 kb on - strand at 3611.538 kb on - strand at 3611.595 kb on + strand at 3611.682 kb on + strand at 3611.682 kb on + strand at 3611.692 kb on + strand at 3611.779 kb on + strand at 3611.787 kb on - strand at 3611.802 kb on + strand at 3611.823 kb on + strand at 3611.823 kb on - strand at 3611.823 kb on - strand at 3611.823 kb on - strand at 3611.831 kb on - strand at 3611.831 kb on - strand at 3611.853 kb on - strand, within nikA at 3611.930 kb on - strand, within nikA at 3611.946 kb on + strand, within nikA at 3611.952 kb on - strand, within nikA at 3611.956 kb on + strand, within nikA at 3611.956 kb on + strand, within nikA at 3612.028 kb on + strand, within nikA at 3612.028 kb on + strand, within nikA at 3612.094 kb on + strand, within nikA at 3612.130 kb on + strand, within nikA at 3612.163 kb on - strand, within nikA at 3612.272 kb on + strand, within nikA at 3612.403 kb on + strand, within nikA at 3612.480 kb on + strand, within nikA at 3612.480 kb on + strand, within nikA at 3612.560 kb on - strand, within nikA at 3612.560 kb on - strand, within nikA at 3612.582 kb on - strand, within nikA at 3612.602 kb on + strand, within nikA at 3612.602 kb on + strand, within nikA at 3612.612 kb on - strand, within nikA at 3612.644 kb on + strand, within nikA at 3612.666 kb on + strand, within nikA at 3612.687 kb on - strand, within nikA at 3612.701 kb on + strand, within nikA at 3612.743 kb on + strand, within nikA at 3612.801 kb on - strand, within nikA at 3612.982 kb on - strand, within nikA at 3613.011 kb on + strand, within nikA at 3613.103 kb on + strand, within nikA at 3613.103 kb on + strand, within nikA at 3613.132 kb on - strand at 3613.165 kb on - strand at 3613.195 kb on + strand at 3613.211 kb on - strand at 3613.211 kb on - strand at 3613.251 kb on - strand at 3613.251 kb on - strand at 3613.252 kb on + strand at 3613.309 kb on + strand at 3613.309 kb on + strand at 3613.360 kb on - strand, within nikB at 3613.403 kb on + strand, within nikB at 3613.403 kb on + strand, within nikB at 3613.445 kb on + strand, within nikB at 3613.450 kb on + strand, within nikB at 3613.461 kb on - strand, within nikB at 3613.461 kb on - strand, within nikB at 3613.467 kb on - strand, within nikB at 3613.531 kb on + strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.539 kb on - strand, within nikB at 3613.544 kb on + strand, within nikB at 3613.551 kb on - strand, within nikB at 3613.592 kb on - strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.607 kb on + strand, within nikB at 3613.615 kb on - strand, within nikB at 3613.615 kb on - strand, within nikB at 3613.670 kb on + strand, within nikB at 3613.670 kb on + strand, within nikB at 3613.691 kb on + strand, within nikB at 3613.773 kb on - strand, within nikB at 3613.796 kb on - strand, within nikB at 3613.848 kb on - strand, within nikB at 3613.848 kb on - strand, within nikB at 3613.869 kb on + strand, within nikB at 3613.874 kb on - strand, within nikB at 3613.878 kb on + strand, within nikB at 3613.881 kb on - strand, within nikB at 3613.932 kb on + strand, within nikB at 3613.934 kb on + strand, within nikB at 3613.942 kb on + strand, within nikB at 3613.942 kb on - strand, within nikB at 3613.947 kb on - strand, within nikB at 3613.947 kb on - strand, within nikB at 3613.988 kb on + strand, within nikB at 3614.094 kb on + strand, within nikB at 3614.249 kb on + strand at 3614.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 3,610,757 + yhhT b3474 0.83 -1.0 3,610,757 + yhhT b3474 0.83 -0.8 3,610,760 - yhhT b3474 0.83 -0.2 3,610,762 + yhhT b3474 0.83 +0.2 3,610,837 + +0.8 3,610,837 + -0.1 3,610,944 + -0.3 3,610,946 + +0.1 3,610,989 + -0.3 3,610,992 - -1.1 3,611,002 - -1.1 3,611,002 - +0.4 3,611,031 - +0.6 3,611,059 + acpT b3475 0.11 -0.5 3,611,104 + acpT b3475 0.19 -0.5 3,611,104 + acpT b3475 0.19 -0.5 3,611,122 + acpT b3475 0.22 +0.0 3,611,140 + acpT b3475 0.25 +0.4 3,611,149 + acpT b3475 0.27 -1.5 3,611,149 + acpT b3475 0.27 +0.6 3,611,200 + acpT b3475 0.35 +0.2 3,611,214 + acpT b3475 0.38 +0.2 3,611,234 + acpT b3475 0.41 +0.5 3,611,234 + acpT b3475 0.41 +0.5 3,611,242 - acpT b3475 0.43 -1.1 3,611,242 - acpT b3475 0.43 +0.4 3,611,320 + acpT b3475 0.56 -0.5 3,611,407 + acpT b3475 0.71 -1.0 3,611,407 + acpT b3475 0.71 -0.2 3,611,409 + acpT b3475 0.71 -0.6 3,611,447 - acpT b3475 0.77 -0.3 3,611,447 - acpT b3475 0.77 -1.0 3,611,455 + acpT b3475 0.79 -1.4 3,611,529 - -0.1 3,611,530 + -2.0 3,611,530 + +0.3 3,611,538 - -0.7 3,611,538 - +1.2 3,611,595 + +0.4 3,611,682 + +0.3 3,611,682 + -1.5 3,611,692 + +1.0 3,611,779 + -0.8 3,611,787 - -0.3 3,611,802 + +0.3 3,611,823 + +0.2 3,611,823 - -0.7 3,611,823 - -1.4 3,611,823 - -1.8 3,611,831 - -0.8 3,611,831 - +0.3 3,611,853 - nikA b3476 0.10 -1.1 3,611,930 - nikA b3476 0.15 -0.3 3,611,946 + nikA b3476 0.16 -0.4 3,611,952 - nikA b3476 0.17 +0.0 3,611,956 + nikA b3476 0.17 -2.0 3,611,956 + nikA b3476 0.17 -0.3 3,612,028 + nikA b3476 0.21 -0.5 3,612,028 + nikA b3476 0.21 -0.2 3,612,094 + nikA b3476 0.26 -0.7 3,612,130 + nikA b3476 0.28 -0.3 3,612,163 - nikA b3476 0.30 -0.3 3,612,272 + nikA b3476 0.37 +0.1 3,612,403 + nikA b3476 0.45 -0.8 3,612,480 + nikA b3476 0.50 -0.7 3,612,480 + nikA b3476 0.50 +0.0 3,612,560 - nikA b3476 0.55 -1.2 3,612,560 - nikA b3476 0.55 +0.4 3,612,582 - nikA b3476 0.57 -1.0 3,612,602 + nikA b3476 0.58 -0.0 3,612,602 + nikA b3476 0.58 -0.9 3,612,612 - nikA b3476 0.59 -0.9 3,612,644 + nikA b3476 0.61 -0.9 3,612,666 + nikA b3476 0.62 -1.8 3,612,687 - nikA b3476 0.63 -1.3 3,612,701 + nikA b3476 0.64 -0.3 3,612,743 + nikA b3476 0.67 -0.4 3,612,801 - nikA b3476 0.71 -1.1 3,612,982 - nikA b3476 0.82 +0.1 3,613,011 + nikA b3476 0.84 -0.4 3,613,103 + nikA b3476 0.90 -1.0 3,613,103 + nikA b3476 0.90 -1.3 3,613,132 - -1.3 3,613,165 - -1.5 3,613,195 + -0.1 3,613,211 - -0.2 3,613,211 - -0.3 3,613,251 - -0.6 3,613,251 - +0.0 3,613,252 + +0.0 3,613,309 + -0.5 3,613,309 + -0.4 3,613,360 - nikB b3477 0.10 -0.8 3,613,403 + nikB b3477 0.15 +1.4 3,613,403 + nikB b3477 0.15 -0.1 3,613,445 + nikB b3477 0.19 -0.5 3,613,450 + nikB b3477 0.20 -0.6 3,613,461 - nikB b3477 0.21 +0.8 3,613,461 - nikB b3477 0.21 -1.5 3,613,467 - nikB b3477 0.21 -1.3 3,613,531 + nikB b3477 0.28 -0.5 3,613,539 - nikB b3477 0.29 -1.5 3,613,539 - nikB b3477 0.29 -0.1 3,613,539 - nikB b3477 0.29 -1.4 3,613,539 - nikB b3477 0.29 -1.2 3,613,539 - nikB b3477 0.29 -0.5 3,613,539 - nikB b3477 0.29 -0.1 3,613,539 - nikB b3477 0.29 +0.0 3,613,544 + nikB b3477 0.30 +0.9 3,613,551 - nikB b3477 0.30 +0.7 3,613,592 - nikB b3477 0.35 -0.9 3,613,607 + nikB b3477 0.36 +0.6 3,613,607 + nikB b3477 0.36 +0.3 3,613,607 + nikB b3477 0.36 -0.7 3,613,615 - nikB b3477 0.37 -1.8 3,613,615 - nikB b3477 0.37 -0.8 3,613,670 + nikB b3477 0.43 -0.4 3,613,670 + nikB b3477 0.43 -1.8 3,613,691 + nikB b3477 0.45 -0.2 3,613,773 - nikB b3477 0.54 -1.1 3,613,796 - nikB b3477 0.56 -2.0 3,613,848 - nikB b3477 0.62 -0.5 3,613,848 - nikB b3477 0.62 -0.3 3,613,869 + nikB b3477 0.64 -0.7 3,613,874 - nikB b3477 0.65 +0.2 3,613,878 + nikB b3477 0.65 -0.0 3,613,881 - nikB b3477 0.65 -0.9 3,613,932 + nikB b3477 0.71 -0.4 3,613,934 + nikB b3477 0.71 -0.7 3,613,942 + nikB b3477 0.72 -0.2 3,613,942 - nikB b3477 0.72 -0.5 3,613,947 - nikB b3477 0.72 -0.5 3,613,947 - nikB b3477 0.72 -1.3 3,613,988 + nikB b3477 0.77 -1.1 3,614,094 + nikB b3477 0.88 -1.0 3,614,249 + +0.5 3,614,249 + -0.3
Or see this region's nucleotide sequence