Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yrbG and kdsD are separated by 13 nucleotides kdsD and kdsC are separated by 20 nucleotides kdsC and yrbK overlap by 4 nucleotides
b3196: yrbG - predicted calcium/sodium:proton antiporter (NCBI), at 3,338,297 to 3,339,274
yrbG
b3197: kdsD - D-arabinose 5-phosphate isomerase (NCBI), at 3,339,288 to 3,340,274
kdsD
b3198: kdsC - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI), at 3,340,295 to 3,340,861
kdsC
b3199: yrbK - hypothetical protein (NCBI), at 3,340,858 to 3,341,433
yrbK
Position (kb)
3339
3340
3341 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3338.300 kb on - strand at 3338.357 kb on - strand at 3338.357 kb on - strand at 3338.436 kb on - strand, within yrbG at 3338.436 kb on - strand, within yrbG at 3338.436 kb on - strand, within yrbG at 3338.436 kb on - strand, within yrbG at 3338.462 kb on + strand, within yrbG at 3338.503 kb on + strand, within yrbG at 3338.508 kb on + strand, within yrbG at 3338.508 kb on + strand, within yrbG at 3338.553 kb on + strand, within yrbG at 3338.591 kb on + strand, within yrbG at 3338.594 kb on + strand, within yrbG at 3338.601 kb on + strand, within yrbG at 3338.612 kb on + strand, within yrbG at 3338.612 kb on + strand, within yrbG at 3338.626 kb on + strand, within yrbG at 3338.629 kb on - strand, within yrbG at 3338.629 kb on - strand, within yrbG at 3338.643 kb on + strand, within yrbG at 3338.646 kb on - strand, within yrbG at 3338.651 kb on - strand, within yrbG at 3338.693 kb on - strand at 3338.714 kb on - strand, within yrbG at 3338.714 kb on - strand, within yrbG at 3338.714 kb on - strand, within yrbG at 3338.722 kb on - strand, within yrbG at 3338.768 kb on - strand, within yrbG at 3338.789 kb on + strand, within yrbG at 3338.792 kb on - strand, within yrbG at 3338.797 kb on - strand, within yrbG at 3338.797 kb on - strand, within yrbG at 3338.876 kb on + strand, within yrbG at 3338.891 kb on + strand, within yrbG at 3338.897 kb on + strand, within yrbG at 3338.960 kb on - strand, within yrbG at 3338.984 kb on - strand, within yrbG at 3339.031 kb on - strand, within yrbG at 3339.092 kb on - strand, within yrbG at 3339.092 kb on - strand, within yrbG at 3339.107 kb on + strand, within yrbG at 3339.107 kb on + strand, within yrbG at 3339.115 kb on - strand, within yrbG at 3339.122 kb on - strand, within yrbG at 3339.122 kb on - strand, within yrbG at 3339.209 kb on - strand at 3339.209 kb on - strand at 3339.239 kb on + strand at 3339.255 kb on + strand at 3339.255 kb on + strand at 3339.301 kb on + strand at 3339.309 kb on - strand at 3339.309 kb on - strand at 3339.309 kb on - strand at 3339.346 kb on + strand at 3339.346 kb on + strand at 3339.350 kb on + strand at 3339.417 kb on - strand, within kdsD at 3339.447 kb on + strand, within kdsD at 3339.516 kb on - strand, within kdsD at 3339.536 kb on + strand, within kdsD at 3339.579 kb on - strand, within kdsD at 3339.579 kb on - strand, within kdsD at 3339.584 kb on + strand, within kdsD at 3339.590 kb on - strand, within kdsD at 3339.596 kb on + strand, within kdsD at 3339.596 kb on + strand, within kdsD at 3339.604 kb on - strand, within kdsD at 3339.635 kb on - strand, within kdsD at 3339.649 kb on - strand, within kdsD at 3339.649 kb on - strand, within kdsD at 3339.719 kb on + strand, within kdsD at 3339.720 kb on - strand, within kdsD at 3339.720 kb on - strand, within kdsD at 3339.773 kb on + strand, within kdsD at 3339.773 kb on + strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.778 kb on - strand, within kdsD at 3339.791 kb on + strand, within kdsD at 3339.791 kb on + strand, within kdsD at 3339.829 kb on - strand, within kdsD at 3339.864 kb on + strand, within kdsD at 3339.868 kb on - strand, within kdsD at 3339.876 kb on + strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.879 kb on - strand, within kdsD at 3339.924 kb on + strand, within kdsD at 3339.974 kb on + strand, within kdsD at 3340.032 kb on + strand, within kdsD at 3340.105 kb on - strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.129 kb on + strand, within kdsD at 3340.203 kb on + strand at 3340.203 kb on + strand at 3340.231 kb on + strand at 3340.269 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.326 kb on + strand at 3340.374 kb on + strand, within kdsC at 3340.374 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.391 kb on + strand, within kdsC at 3340.736 kb on + strand, within kdsC at 3341.218 kb on + strand, within yrbK at 3341.218 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK at 3341.256 kb on + strand, within yrbK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 3,338,300 - -0.0 3,338,357 - +0.7 3,338,357 - -0.5 3,338,436 - yrbG b3196 0.14 +0.2 3,338,436 - yrbG b3196 0.14 -0.7 3,338,436 - yrbG b3196 0.14 +0.0 3,338,436 - yrbG b3196 0.14 -0.1 3,338,462 + yrbG b3196 0.17 -1.3 3,338,503 + yrbG b3196 0.21 -1.5 3,338,508 + yrbG b3196 0.22 +0.4 3,338,508 + yrbG b3196 0.22 +0.3 3,338,553 + yrbG b3196 0.26 +0.2 3,338,591 + yrbG b3196 0.30 -0.7 3,338,594 + yrbG b3196 0.30 -0.3 3,338,601 + yrbG b3196 0.31 -0.5 3,338,612 + yrbG b3196 0.32 +0.5 3,338,612 + yrbG b3196 0.32 -1.8 3,338,626 + yrbG b3196 0.34 -0.6 3,338,629 - yrbG b3196 0.34 +0.2 3,338,629 - yrbG b3196 0.34 +1.7 3,338,643 + yrbG b3196 0.35 -0.1 3,338,646 - yrbG b3196 0.36 +0.4 3,338,651 - yrbG b3196 0.36 -0.1 3,338,693 - -1.1 3,338,714 - yrbG b3196 0.43 +0.7 3,338,714 - yrbG b3196 0.43 +0.6 3,338,714 - yrbG b3196 0.43 +0.7 3,338,722 - yrbG b3196 0.43 -1.0 3,338,768 - yrbG b3196 0.48 -1.1 3,338,789 + yrbG b3196 0.50 -0.3 3,338,792 - yrbG b3196 0.51 +0.2 3,338,797 - yrbG b3196 0.51 +0.6 3,338,797 - yrbG b3196 0.51 +0.4 3,338,876 + yrbG b3196 0.59 +0.1 3,338,891 + yrbG b3196 0.61 +0.9 3,338,897 + yrbG b3196 0.61 -0.8 3,338,960 - yrbG b3196 0.68 +0.6 3,338,984 - yrbG b3196 0.70 -0.8 3,339,031 - yrbG b3196 0.75 +0.2 3,339,092 - yrbG b3196 0.81 +1.1 3,339,092 - yrbG b3196 0.81 +0.7 3,339,107 + yrbG b3196 0.83 -0.9 3,339,107 + yrbG b3196 0.83 -0.6 3,339,115 - yrbG b3196 0.84 -0.7 3,339,122 - yrbG b3196 0.84 -0.8 3,339,122 - yrbG b3196 0.84 +0.1 3,339,209 - +0.7 3,339,209 - -1.8 3,339,239 + -0.3 3,339,255 + +0.6 3,339,255 + -3.3 3,339,301 + -0.8 3,339,309 - +0.5 3,339,309 - -0.1 3,339,309 - -0.0 3,339,346 + -0.5 3,339,346 + -1.3 3,339,350 + -1.4 3,339,417 - kdsD b3197 0.13 -1.0 3,339,447 + kdsD b3197 0.16 -1.5 3,339,516 - kdsD b3197 0.23 +0.3 3,339,536 + kdsD b3197 0.25 -1.7 3,339,579 - kdsD b3197 0.29 -0.9 3,339,579 - kdsD b3197 0.29 -0.7 3,339,584 + kdsD b3197 0.30 -0.4 3,339,590 - kdsD b3197 0.31 +0.9 3,339,596 + kdsD b3197 0.31 -0.8 3,339,596 + kdsD b3197 0.31 -0.7 3,339,604 - kdsD b3197 0.32 -0.1 3,339,635 - kdsD b3197 0.35 -0.7 3,339,649 - kdsD b3197 0.37 -2.5 3,339,649 - kdsD b3197 0.37 -0.7 3,339,719 + kdsD b3197 0.44 -2.0 3,339,720 - kdsD b3197 0.44 -1.0 3,339,720 - kdsD b3197 0.44 -1.3 3,339,773 + kdsD b3197 0.49 -1.1 3,339,773 + kdsD b3197 0.49 -1.5 3,339,778 - kdsD b3197 0.50 -0.7 3,339,778 - kdsD b3197 0.50 -1.0 3,339,778 - kdsD b3197 0.50 -0.5 3,339,791 + kdsD b3197 0.51 -0.1 3,339,791 + kdsD b3197 0.51 -2.1 3,339,829 - kdsD b3197 0.55 -0.6 3,339,864 + kdsD b3197 0.58 +0.2 3,339,868 - kdsD b3197 0.59 -0.1 3,339,876 + kdsD b3197 0.60 -0.2 3,339,879 - kdsD b3197 0.60 +0.0 3,339,879 - kdsD b3197 0.60 +0.4 3,339,924 + kdsD b3197 0.64 -2.3 3,339,974 + kdsD b3197 0.70 -0.7 3,340,032 + kdsD b3197 0.75 -0.8 3,340,105 - kdsD b3197 0.83 +0.9 3,340,129 + kdsD b3197 0.85 -1.5 3,340,129 + kdsD b3197 0.85 -1.1 3,340,203 + -0.7 3,340,203 + -0.7 3,340,231 + -0.6 3,340,269 + -0.6 3,340,326 + -0.5 3,340,326 + +0.1 3,340,326 + -2.7 3,340,374 + kdsC b3198 0.14 +0.7 3,340,374 + kdsC b3198 0.14 -0.5 3,340,391 + kdsC b3198 0.17 +0.9 3,340,391 + kdsC b3198 0.17 -0.5 3,340,736 + kdsC b3198 0.78 +0.2 3,341,218 + yrbK b3199 0.62 -0.1 3,341,218 + yrbK b3199 0.62 +0.9 3,341,256 + yrbK b3199 0.69 -1.8 3,341,256 + yrbK b3199 0.69 -2.8
Or see this region's nucleotide sequence