Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybB and hybA overlap by 11 nucleotides hybA and hybO are separated by 2 nucleotides hybO and yghW are separated by 188 nucleotides yghW and yghX are separated by 118 nucleotides
b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186
hybB
b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162
hybA
b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283
hybO
b2998: yghW - hypothetical protein (NCBI), at 3,144,472 to 3,144,759
yghW
b4658: yghX - no description, at 3,144,878 to 3,145,713
yghX
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3142.271 kb on - strand at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.368 kb on + strand, within hybA at 3142.410 kb on + strand, within hybA at 3142.446 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.587 kb on + strand, within hybA at 3142.600 kb on + strand, within hybA at 3142.676 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.865 kb on + strand, within hybA at 3142.877 kb on + strand, within hybA at 3142.885 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3143.008 kb on + strand, within hybA at 3143.108 kb on - strand at 3143.108 kb on - strand at 3143.127 kb on + strand at 3143.127 kb on + strand at 3143.223 kb on + strand at 3143.230 kb on + strand at 3143.230 kb on + strand at 3143.277 kb on - strand, within hybO at 3143.279 kb on - strand, within hybO at 3143.410 kb on - strand, within hybO at 3143.410 kb on - strand, within hybO at 3143.456 kb on + strand, within hybO at 3143.471 kb on - strand, within hybO at 3143.475 kb on + strand, within hybO at 3143.499 kb on - strand, within hybO at 3143.515 kb on - strand, within hybO at 3143.521 kb on - strand, within hybO at 3143.656 kb on + strand, within hybO at 3143.677 kb on - strand at 3143.677 kb on - strand, within hybO at 3143.799 kb on - strand, within hybO at 3143.861 kb on - strand, within hybO at 3143.897 kb on + strand, within hybO at 3143.960 kb on - strand, within hybO at 3143.995 kb on + strand, within hybO at 3144.007 kb on + strand, within hybO at 3144.091 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO at 3144.201 kb on + strand at 3144.202 kb on - strand at 3144.250 kb on + strand at 3144.259 kb on + strand at 3144.259 kb on + strand at 3144.267 kb on + strand at 3144.269 kb on + strand at 3144.269 kb on + strand at 3144.395 kb on + strand at 3144.395 kb on + strand at 3144.398 kb on - strand at 3144.437 kb on - strand at 3144.485 kb on + strand at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.560 kb on - strand, within yghW at 3144.561 kb on - strand, within yghW at 3144.564 kb on + strand, within yghW at 3144.564 kb on + strand, within yghW at 3144.659 kb on - strand, within yghW at 3144.701 kb on - strand, within yghW at 3144.845 kb on + strand at 3144.854 kb on - strand at 3144.854 kb on - strand at 3144.911 kb on + strand at 3144.992 kb on + strand, within yghX at 3144.992 kb on + strand, within yghX at 3145.000 kb on - strand, within yghX at 3145.000 kb on - strand, within yghX at 3145.009 kb on + strand, within yghX at 3145.010 kb on - strand, within yghX at 3145.034 kb on + strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.052 kb on - strand, within yghX at 3145.080 kb on + strand, within yghX at 3145.085 kb on + strand, within yghX at 3145.152 kb on + strand, within yghX at 3145.157 kb on + strand, within yghX at 3145.240 kb on + strand, within yghX
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 3,142,271 - -0.8 3,142,284 - hybA b2996 0.11 -0.1 3,142,284 - hybA b2996 0.11 -1.4 3,142,284 - hybA b2996 0.11 +0.5 3,142,286 - hybA b2996 0.11 -0.8 3,142,286 - hybA b2996 0.11 -0.2 3,142,368 + hybA b2996 0.19 -1.8 3,142,410 + hybA b2996 0.24 +0.6 3,142,446 - hybA b2996 0.27 -0.9 3,142,585 - hybA b2996 0.41 -2.0 3,142,585 - hybA b2996 0.41 -0.4 3,142,587 + hybA b2996 0.42 -1.0 3,142,600 + hybA b2996 0.43 -0.6 3,142,676 - hybA b2996 0.51 -0.3 3,142,719 - hybA b2996 0.55 -0.6 3,142,719 - hybA b2996 0.55 -1.0 3,142,865 + hybA b2996 0.70 -0.5 3,142,877 + hybA b2996 0.71 -0.2 3,142,885 - hybA b2996 0.72 -1.3 3,142,995 - hybA b2996 0.83 -1.1 3,142,995 - hybA b2996 0.83 -0.8 3,143,008 + hybA b2996 0.84 -1.3 3,143,108 - +0.2 3,143,108 - +0.1 3,143,127 + -0.5 3,143,127 + -1.1 3,143,223 + -0.8 3,143,230 + -0.2 3,143,230 + -0.8 3,143,277 - hybO b2997 0.10 -0.7 3,143,279 - hybO b2997 0.10 -1.6 3,143,410 - hybO b2997 0.22 -1.1 3,143,410 - hybO b2997 0.22 -0.0 3,143,456 + hybO b2997 0.26 -0.2 3,143,471 - hybO b2997 0.27 -1.5 3,143,475 + hybO b2997 0.28 -0.3 3,143,499 - hybO b2997 0.30 -1.4 3,143,515 - hybO b2997 0.31 -0.4 3,143,521 - hybO b2997 0.32 -1.1 3,143,656 + hybO b2997 0.44 -0.5 3,143,677 - -1.2 3,143,677 - hybO b2997 0.46 +1.2 3,143,799 - hybO b2997 0.57 -1.1 3,143,861 - hybO b2997 0.62 -0.2 3,143,897 + hybO b2997 0.65 -0.4 3,143,960 - hybO b2997 0.71 -0.2 3,143,995 + hybO b2997 0.74 -0.7 3,144,007 + hybO b2997 0.75 -0.8 3,144,091 + hybO b2997 0.83 -1.0 3,144,105 + hybO b2997 0.84 -0.1 3,144,105 + hybO b2997 0.84 -1.1 3,144,201 + -0.2 3,144,202 - -0.2 3,144,250 + -0.1 3,144,259 + -0.2 3,144,259 + -1.3 3,144,267 + -0.8 3,144,269 + -0.4 3,144,269 + -0.9 3,144,395 + +0.1 3,144,395 + +0.0 3,144,398 - +0.7 3,144,437 - -0.5 3,144,485 + -2.2 3,144,553 + yghW b2998 0.28 +0.3 3,144,553 + yghW b2998 0.28 -1.5 3,144,553 + yghW b2998 0.28 -0.2 3,144,553 + yghW b2998 0.28 +0.8 3,144,553 + yghW b2998 0.28 -1.8 3,144,560 - yghW b2998 0.31 -0.7 3,144,561 - yghW b2998 0.31 -0.2 3,144,564 + yghW b2998 0.32 -0.6 3,144,564 + yghW b2998 0.32 -0.1 3,144,659 - yghW b2998 0.65 -0.8 3,144,701 - yghW b2998 0.80 +0.1 3,144,845 + +0.7 3,144,854 - -0.2 3,144,854 - -0.5 3,144,911 + -0.2 3,144,992 + yghX b4658 0.14 +1.5 3,144,992 + yghX b4658 0.14 +0.2 3,145,000 - yghX b4658 0.15 -0.2 3,145,000 - yghX b4658 0.15 -0.3 3,145,009 + yghX b4658 0.16 +1.0 3,145,010 - yghX b4658 0.16 +0.1 3,145,034 + yghX b4658 0.19 -1.5 3,145,042 - yghX b4658 0.20 +1.8 3,145,042 - yghX b4658 0.20 -1.5 3,145,042 - yghX b4658 0.20 -0.2 3,145,042 - yghX b4658 0.20 +0.1 3,145,052 - yghX b4658 0.21 +0.4 3,145,080 + yghX b4658 0.24 -1.9 3,145,085 + yghX b4658 0.25 -0.6 3,145,152 + yghX b4658 0.33 -0.3 3,145,157 + yghX b4658 0.33 -2.4 3,145,240 + yghX b4658 0.43 +0.0
Or see this region's nucleotide sequence