Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybbS and allA are separated by 229 nucleotides allA and allR are separated by 77 nucleotides allR and gcl are separated by 89 nucleotides
b0504: ybbS - DNA-binding transcriptional activator of the allD operon (NCBI), at 530,519 to 531,445
ybbS
b0505: allA - ureidoglycolate hydrolase (NCBI), at 531,675 to 532,157
allA
b0506: allR - DNA-binding transcriptional repressor (NCBI), at 532,235 to 533,050
allR
b0507: gcl - glyoxylate carboligase (NCBI), at 533,140 to 534,921
gcl
Position (kb) 532
533
534 Strain fitness (log2 ratio) -2
-1
0
1 at 531.687 kb on + strand at 531.687 kb on + strand at 531.776 kb on + strand, within allA at 531.816 kb on + strand, within allA at 531.907 kb on - strand, within allA at 531.962 kb on - strand, within allA at 532.199 kb on + strand at 532.216 kb on - strand at 532.221 kb on + strand at 532.278 kb on + strand at 532.278 kb on + strand at 532.497 kb on - strand, within allR at 532.513 kb on - strand, within allR at 532.560 kb on + strand, within allR at 532.616 kb on + strand, within allR at 532.642 kb on - strand, within allR at 532.657 kb on - strand, within allR at 532.677 kb on + strand, within allR at 532.677 kb on + strand, within allR at 532.798 kb on - strand, within allR at 532.798 kb on - strand, within allR at 532.811 kb on + strand, within allR at 532.811 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.992 kb on - strand at 533.167 kb on + strand at 533.167 kb on + strand at 533.171 kb on + strand at 533.171 kb on + strand at 533.245 kb on + strand at 533.246 kb on - strand at 533.423 kb on + strand, within gcl at 533.444 kb on + strand, within gcl at 533.561 kb on - strand, within gcl at 533.606 kb on + strand, within gcl at 533.754 kb on + strand, within gcl at 533.754 kb on + strand, within gcl at 533.805 kb on + strand, within gcl at 533.859 kb on - strand, within gcl at 533.869 kb on + strand, within gcl at 533.881 kb on + strand, within gcl at 533.922 kb on + strand, within gcl at 533.996 kb on - strand, within gcl
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 531,687 + +0.6 531,687 + +0.8 531,776 + allA b0505 0.21 +0.5 531,816 + allA b0505 0.29 -0.5 531,907 - allA b0505 0.48 -0.0 531,962 - allA b0505 0.59 +0.5 532,199 + -0.2 532,216 - -0.2 532,221 + +1.1 532,278 + -0.6 532,278 + -0.5 532,497 - allR b0506 0.32 -0.5 532,513 - allR b0506 0.34 -0.8 532,560 + allR b0506 0.40 -0.2 532,616 + allR b0506 0.47 -0.4 532,642 - allR b0506 0.50 -1.1 532,657 - allR b0506 0.52 -0.5 532,677 + allR b0506 0.54 -1.2 532,677 + allR b0506 0.54 -2.4 532,798 - allR b0506 0.69 -1.0 532,798 - allR b0506 0.69 -0.2 532,811 + allR b0506 0.71 -0.7 532,811 + allR b0506 0.71 -0.0 532,840 + allR b0506 0.74 -0.4 532,840 + allR b0506 0.74 +0.8 532,992 - -1.4 533,167 + +0.0 533,167 + -0.1 533,171 + -1.1 533,171 + -0.7 533,245 + +1.1 533,246 - -0.1 533,423 + gcl b0507 0.16 +1.6 533,444 + gcl b0507 0.17 +0.2 533,561 - gcl b0507 0.24 +0.1 533,606 + gcl b0507 0.26 -1.0 533,754 + gcl b0507 0.34 -0.4 533,754 + gcl b0507 0.34 +0.7 533,805 + gcl b0507 0.37 +0.2 533,859 - gcl b0507 0.40 -0.9 533,869 + gcl b0507 0.41 +1.7 533,881 + gcl b0507 0.42 +0.0 533,922 + gcl b0507 0.44 -0.1 533,996 - gcl b0507 0.48 -0.2
Or see this region's nucleotide sequence