Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2021

Experiment: KB with Avocatin B 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2019 and Psyr_2020 are separated by 10 nucleotidesPsyr_2020 and Psyr_2021 are separated by 221 nucleotidesPsyr_2021 and Psyr_2022 are separated by 284 nucleotides Psyr_2019: Psyr_2019 - 3-oxoacyl-[acyl-carrier-protein] synthase I, at 2,343,838 to 2,345,061 _2019 Psyr_2020: Psyr_2020 - 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase, at 2,345,072 to 2,345,587 _2020 Psyr_2021: Psyr_2021 - Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal, at 2,345,809 to 2,347,746 _2021 Psyr_2022: Psyr_2022 - Glycerol-3-phosphate dehydrogenase (NAD(P)+), at 2,348,031 to 2,349,056 _2022 Position (kb) 2345 2346 2347 2348Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2345.657 kb on - strandat 2345.686 kb on - strandat 2345.692 kb on - strandat 2345.692 kb on - strandat 2345.858 kb on - strandat 2345.870 kb on - strandat 2345.901 kb on - strandat 2346.020 kb on + strand, within Psyr_2021at 2346.241 kb on - strand, within Psyr_2021at 2346.320 kb on + strand, within Psyr_2021at 2346.320 kb on + strand, within Psyr_2021at 2346.320 kb on + strand, within Psyr_2021at 2346.320 kb on + strand, within Psyr_2021at 2346.321 kb on - strand, within Psyr_2021at 2346.321 kb on - strand, within Psyr_2021at 2346.433 kb on - strand, within Psyr_2021at 2346.486 kb on + strand, within Psyr_2021at 2346.486 kb on + strand, within Psyr_2021at 2346.487 kb on - strand, within Psyr_2021at 2346.558 kb on + strand, within Psyr_2021at 2346.559 kb on - strand, within Psyr_2021at 2346.559 kb on - strand, within Psyr_2021at 2346.559 kb on - strand, within Psyr_2021at 2346.614 kb on - strand, within Psyr_2021at 2346.614 kb on - strand, within Psyr_2021at 2347.109 kb on + strand, within Psyr_2021at 2347.110 kb on - strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.244 kb on - strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.304 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.450 kb on + strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.451 kb on - strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.454 kb on + strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.455 kb on - strand, within Psyr_2021at 2347.468 kb on - strand, within Psyr_2021at 2347.493 kb on + strand, within Psyr_2021at 2347.493 kb on + strand, within Psyr_2021at 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.641 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.645 kb on + strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.646 kb on - strandat 2347.653 kb on + strandat 2347.654 kb on + strandat 2347.722 kb on + strandat 2347.722 kb on + strandat 2347.985 kb on + strandat 2347.986 kb on - strandat 2347.988 kb on - strandat 2348.030 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Avocatin B 10 mM
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2,345,657 - +1.6
2,345,686 - +0.5
2,345,692 - -0.5
2,345,692 - -0.4
2,345,858 - +3.2
2,345,870 - +0.7
2,345,901 - +0.5
2,346,020 + Psyr_2021 0.11 -2.0
2,346,241 - Psyr_2021 0.22 -1.0
2,346,320 + Psyr_2021 0.26 -0.3
2,346,320 + Psyr_2021 0.26 +0.9
2,346,320 + Psyr_2021 0.26 -1.2
2,346,320 + Psyr_2021 0.26 -1.5
2,346,321 - Psyr_2021 0.26 -0.6
2,346,321 - Psyr_2021 0.26 +0.6
2,346,433 - Psyr_2021 0.32 +2.0
2,346,486 + Psyr_2021 0.35 +0.1
2,346,486 + Psyr_2021 0.35 -1.3
2,346,487 - Psyr_2021 0.35 +0.6
2,346,558 + Psyr_2021 0.39 +0.6
2,346,559 - Psyr_2021 0.39 +1.2
2,346,559 - Psyr_2021 0.39 +0.2
2,346,559 - Psyr_2021 0.39 +0.2
2,346,614 - Psyr_2021 0.42 +2.0
2,346,614 - Psyr_2021 0.42 +1.2
2,347,109 + Psyr_2021 0.67 +3.2
2,347,110 - Psyr_2021 0.67 +1.8
2,347,244 - Psyr_2021 0.74 +0.9
2,347,244 - Psyr_2021 0.74 -0.7
2,347,304 + Psyr_2021 0.77 -0.4
2,347,304 + Psyr_2021 0.77 +1.5
2,347,304 + Psyr_2021 0.77 +0.5
2,347,450 + Psyr_2021 0.85 +0.6
2,347,450 + Psyr_2021 0.85 +2.4
2,347,450 + Psyr_2021 0.85 -1.4
2,347,451 - Psyr_2021 0.85 -0.1
2,347,451 - Psyr_2021 0.85 +0.9
2,347,451 - Psyr_2021 0.85 -1.0
2,347,451 - Psyr_2021 0.85 +1.1
2,347,451 - Psyr_2021 0.85 +0.6
2,347,454 + Psyr_2021 0.85 +1.5
2,347,454 + Psyr_2021 0.85 -0.7
2,347,454 + Psyr_2021 0.85 -0.4
2,347,454 + Psyr_2021 0.85 +1.5
2,347,454 + Psyr_2021 0.85 +0.6
2,347,454 + Psyr_2021 0.85 +0.3
2,347,454 + Psyr_2021 0.85 -0.3
2,347,454 + Psyr_2021 0.85 +0.1
2,347,454 + Psyr_2021 0.85 -0.4
2,347,455 - Psyr_2021 0.85 +1.2
2,347,455 - Psyr_2021 0.85 +1.1
2,347,455 - Psyr_2021 0.85 +0.9
2,347,455 - Psyr_2021 0.85 +1.0
2,347,455 - Psyr_2021 0.85 +0.6
2,347,455 - Psyr_2021 0.85 +0.4
2,347,455 - Psyr_2021 0.85 +0.6
2,347,455 - Psyr_2021 0.85 +0.6
2,347,468 - Psyr_2021 0.86 -1.4
2,347,493 + Psyr_2021 0.87 +0.5
2,347,493 + Psyr_2021 0.87 +0.3
2,347,641 + -0.3
2,347,641 + +0.2
2,347,641 + +0.3
2,347,641 + -0.3
2,347,645 + -0.1
2,347,645 + +1.2
2,347,645 + +0.1
2,347,645 + +1.3
2,347,645 + +0.9
2,347,645 + -0.4
2,347,646 - +0.5
2,347,646 - -0.3
2,347,646 - +0.3
2,347,646 - +0.4
2,347,646 - +1.2
2,347,653 + -0.2
2,347,654 + -0.1
2,347,722 + -0.1
2,347,722 + +1.2
2,347,985 + +1.2
2,347,986 - +0.2
2,347,988 - +0.2
2,348,030 + +0.8

Or see this region's nucleotide sequence