Experiment: KB with Veratrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2464 and Psyr_2465 are separated by 406 nucleotides Psyr_2465 and Psyr_2466 are separated by 70 nucleotides Psyr_2466 and Psyr_2467 are separated by 96 nucleotides
Psyr_2464: Psyr_2464 - methionine synthase (B12-dependent), at 2,852,906 to 2,856,625
_2464
Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054
_2465
Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718
_2466
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Position (kb)
2857
2858
2859 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2856.688 kb on + strand at 2856.746 kb on + strand at 2856.746 kb on + strand at 2856.747 kb on - strand at 2856.747 kb on - strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.802 kb on - strand at 2856.924 kb on + strand at 2856.975 kb on + strand at 2856.975 kb on + strand at 2857.014 kb on + strand at 2857.014 kb on + strand at 2857.015 kb on - strand at 2857.278 kb on + strand, within Psyr_2465 at 2857.286 kb on - strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.288 kb on - strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.464 kb on + strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.483 kb on + strand, within Psyr_2465 at 2857.483 kb on + strand, within Psyr_2465 at 2857.484 kb on - strand, within Psyr_2465 at 2857.484 kb on - strand, within Psyr_2465 at 2857.599 kb on + strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.699 kb on - strand, within Psyr_2465 at 2857.791 kb on + strand, within Psyr_2465 at 2857.836 kb on + strand, within Psyr_2465 at 2857.845 kb on + strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.965 kb on + strand at 2857.965 kb on + strand at 2857.966 kb on - strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.714 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Veratrine 10 mM remove 2,856,688 + +1.4 2,856,746 + +0.5 2,856,746 + -0.4 2,856,747 - +0.7 2,856,747 - +0.7 2,856,754 + -0.7 2,856,754 + +1.3 2,856,754 + -0.9 2,856,754 + -0.3 2,856,802 - +0.3 2,856,924 + +0.2 2,856,975 + +0.2 2,856,975 + -0.5 2,857,014 + -0.5 2,857,014 + -0.2 2,857,015 - -0.1 2,857,278 + Psyr_2465 0.24 +1.7 2,857,286 - Psyr_2465 0.25 +0.6 2,857,287 + Psyr_2465 0.25 -0.3 2,857,287 + Psyr_2465 0.25 +1.0 2,857,287 + Psyr_2465 0.25 +1.1 2,857,287 + Psyr_2465 0.25 +0.7 2,857,288 - Psyr_2465 0.25 +1.4 2,857,449 + Psyr_2465 0.41 +1.6 2,857,449 + Psyr_2465 0.41 -0.5 2,857,449 + Psyr_2465 0.41 +0.5 2,857,449 + Psyr_2465 0.41 -2.6 2,857,449 + Psyr_2465 0.41 +0.8 2,857,450 - Psyr_2465 0.41 +0.5 2,857,450 - Psyr_2465 0.41 +0.1 2,857,450 - Psyr_2465 0.41 -0.5 2,857,464 + Psyr_2465 0.42 +0.4 2,857,465 - Psyr_2465 0.42 +0.1 2,857,465 - Psyr_2465 0.42 -2.3 2,857,465 - Psyr_2465 0.42 -1.0 2,857,483 + Psyr_2465 0.44 -0.7 2,857,483 + Psyr_2465 0.44 +1.6 2,857,484 - Psyr_2465 0.44 -0.0 2,857,484 - Psyr_2465 0.44 -0.3 2,857,599 + Psyr_2465 0.55 +1.5 2,857,600 - Psyr_2465 0.56 -0.5 2,857,600 - Psyr_2465 0.56 -0.6 2,857,600 - Psyr_2465 0.56 -3.5 2,857,699 - Psyr_2465 0.65 +0.4 2,857,791 + Psyr_2465 0.74 +0.2 2,857,836 + Psyr_2465 0.79 +0.7 2,857,845 + Psyr_2465 0.79 -0.6 2,857,912 - Psyr_2465 0.86 -0.1 2,857,912 - Psyr_2465 0.86 -1.6 2,857,912 - Psyr_2465 0.86 +0.2 2,857,965 + -0.3 2,857,965 + +0.9 2,857,966 - +2.0 2,858,713 + -0.4 2,858,713 + +0.2 2,858,713 + -3.8 2,858,713 + +1.4 2,858,714 - -0.8
Or see this region's nucleotide sequence