Experiment: McCas_formate_complete Novobiocin 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02340 and MMJJ_RS02345 are separated by 349 nucleotides MMJJ_RS02345 and MMJJ_RS02350 are separated by 208 nucleotides
MMJJ_RS02340: MMJJ_RS02340 - SAM-dependent methyltransferase, at 442,590 to 443,138
_RS02340
MMJJ_RS02345: MMJJ_RS02345 - ATP-binding protein, at 443,488 to 444,759
_RS02345
MMJJ_RS02350: MMJJ_RS02350 - Fic family protein, at 444,968 to 446,257
_RS02350
Position (kb) 443
444
445 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 442.532 kb on + strand at 442.536 kb on + strand at 442.536 kb on + strand at 442.546 kb on + strand at 442.592 kb on + strand at 442.614 kb on - strand at 442.619 kb on - strand at 442.620 kb on + strand at 442.670 kb on + strand, within MMJJ_RS02340 at 442.670 kb on + strand, within MMJJ_RS02340 at 442.740 kb on - strand, within MMJJ_RS02340 at 442.740 kb on - strand, within MMJJ_RS02340 at 442.740 kb on - strand, within MMJJ_RS02340 at 442.740 kb on - strand at 442.740 kb on - strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.741 kb on + strand, within MMJJ_RS02340 at 442.765 kb on + strand, within MMJJ_RS02340 at 442.765 kb on + strand, within MMJJ_RS02340 at 442.906 kb on - strand, within MMJJ_RS02340 at 442.906 kb on - strand, within MMJJ_RS02340 at 442.906 kb on - strand, within MMJJ_RS02340 at 442.907 kb on + strand, within MMJJ_RS02340 at 443.078 kb on - strand, within MMJJ_RS02340 at 443.079 kb on + strand, within MMJJ_RS02340 at 443.082 kb on + strand, within MMJJ_RS02340 at 443.082 kb on + strand, within MMJJ_RS02340 at 443.214 kb on + strand at 443.218 kb on - strand at 443.236 kb on - strand at 443.236 kb on - strand at 443.237 kb on + strand at 443.238 kb on - strand at 443.238 kb on - strand at 443.239 kb on + strand at 443.240 kb on - strand at 443.240 kb on - strand at 443.288 kb on + strand at 443.301 kb on + strand at 443.337 kb on - strand at 443.350 kb on - strand at 443.354 kb on - strand at 443.355 kb on + strand at 443.384 kb on - strand at 443.384 kb on - strand at 443.394 kb on + strand at 443.398 kb on - strand at 443.402 kb on - strand at 443.463 kb on - strand at 443.463 kb on - strand at 443.463 kb on - strand at 443.463 kb on - strand at 443.463 kb on - strand at 443.464 kb on + strand at 443.464 kb on + strand at 443.464 kb on + strand at 443.464 kb on + strand at 443.464 kb on + strand at 443.464 kb on + strand at 443.465 kb on - strand at 443.465 kb on - strand at 443.465 kb on - strand at 443.465 kb on - strand at 443.466 kb on + strand at 443.466 kb on + strand at 443.467 kb on - strand at 443.467 kb on - strand at 443.468 kb on + strand at 443.468 kb on + strand at 443.468 kb on + strand at 443.472 kb on + strand at 443.490 kb on + strand at 443.494 kb on + strand at 443.537 kb on + strand at 443.538 kb on - strand at 443.539 kb on + strand at 443.564 kb on + strand at 443.583 kb on + strand at 443.584 kb on - strand at 443.585 kb on + strand at 443.742 kb on + strand, within MMJJ_RS02345 at 443.807 kb on - strand, within MMJJ_RS02345 at 443.807 kb on - strand, within MMJJ_RS02345 at 443.808 kb on + strand, within MMJJ_RS02345 at 444.001 kb on + strand, within MMJJ_RS02345 at 444.001 kb on + strand, within MMJJ_RS02345 at 444.007 kb on + strand, within MMJJ_RS02345 at 444.030 kb on - strand, within MMJJ_RS02345 at 444.059 kb on + strand, within MMJJ_RS02345 at 444.059 kb on + strand, within MMJJ_RS02345 at 444.073 kb on - strand, within MMJJ_RS02345 at 444.104 kb on - strand, within MMJJ_RS02345 at 444.105 kb on + strand, within MMJJ_RS02345 at 444.123 kb on - strand, within MMJJ_RS02345 at 444.125 kb on - strand, within MMJJ_RS02345 at 444.126 kb on + strand, within MMJJ_RS02345 at 444.135 kb on - strand, within MMJJ_RS02345 at 444.143 kb on - strand, within MMJJ_RS02345 at 444.209 kb on + strand, within MMJJ_RS02345 at 444.225 kb on - strand, within MMJJ_RS02345 at 444.225 kb on - strand, within MMJJ_RS02345 at 444.238 kb on + strand, within MMJJ_RS02345 at 444.238 kb on + strand, within MMJJ_RS02345 at 444.275 kb on + strand, within MMJJ_RS02345 at 444.275 kb on + strand, within MMJJ_RS02345 at 444.282 kb on - strand, within MMJJ_RS02345 at 444.293 kb on - strand, within MMJJ_RS02345 at 444.317 kb on + strand, within MMJJ_RS02345 at 444.328 kb on - strand, within MMJJ_RS02345 at 444.328 kb on - strand, within MMJJ_RS02345 at 444.329 kb on + strand, within MMJJ_RS02345 at 444.402 kb on - strand, within MMJJ_RS02345 at 444.433 kb on + strand, within MMJJ_RS02345 at 444.490 kb on + strand, within MMJJ_RS02345 at 444.517 kb on - strand, within MMJJ_RS02345 at 444.554 kb on + strand, within MMJJ_RS02345 at 444.682 kb on - strand at 444.682 kb on - strand at 444.683 kb on + strand at 444.697 kb on - strand at 444.704 kb on + strand at 444.704 kb on + strand at 444.719 kb on - strand at 444.726 kb on - strand at 444.837 kb on + strand at 444.873 kb on - strand at 444.873 kb on - strand at 444.878 kb on - strand at 444.899 kb on - strand at 444.918 kb on + strand at 444.919 kb on - strand at 444.920 kb on + strand at 444.956 kb on + strand at 444.962 kb on - strand at 445.021 kb on - strand at 445.045 kb on + strand at 445.052 kb on + strand at 445.064 kb on - strand at 445.102 kb on - strand, within MMJJ_RS02350 at 445.130 kb on + strand, within MMJJ_RS02350 at 445.153 kb on - strand, within MMJJ_RS02350 at 445.198 kb on - strand, within MMJJ_RS02350 at 445.199 kb on + strand, within MMJJ_RS02350 at 445.203 kb on + strand, within MMJJ_RS02350 at 445.308 kb on + strand, within MMJJ_RS02350 at 445.309 kb on - strand, within MMJJ_RS02350 at 445.309 kb on - strand, within MMJJ_RS02350 at 445.309 kb on - strand, within MMJJ_RS02350 at 445.309 kb on - strand, within MMJJ_RS02350 at 445.309 kb on - strand, within MMJJ_RS02350 at 445.310 kb on + strand, within MMJJ_RS02350 at 445.310 kb on + strand, within MMJJ_RS02350 at 445.310 kb on + strand, within MMJJ_RS02350 at 445.310 kb on + strand, within MMJJ_RS02350 at 445.310 kb on + strand, within MMJJ_RS02350 at 445.314 kb on - strand, within MMJJ_RS02350 at 445.315 kb on + strand, within MMJJ_RS02350 at 445.369 kb on - strand, within MMJJ_RS02350 at 445.370 kb on + strand, within MMJJ_RS02350 at 445.464 kb on + strand, within MMJJ_RS02350 at 445.484 kb on + strand, within MMJJ_RS02350 at 445.595 kb on - strand, within MMJJ_RS02350 at 445.634 kb on - strand, within MMJJ_RS02350 at 445.665 kb on - strand, within MMJJ_RS02350 at 445.665 kb on - strand, within MMJJ_RS02350
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Novobiocin 0.0125 mM remove 442,532 + -1.4 442,536 + +2.5 442,536 + +0.4 442,546 + -3.3 442,592 + +2.1 442,614 - -0.1 442,619 - -1.3 442,620 + +2.4 442,670 + MMJJ_RS02340 0.15 +0.7 442,670 + MMJJ_RS02340 0.15 +0.6 442,740 - MMJJ_RS02340 0.27 +0.8 442,740 - MMJJ_RS02340 0.27 +1.1 442,740 - MMJJ_RS02340 0.27 +2.0 442,740 - +0.1 442,740 - MMJJ_RS02340 0.27 -2.4 442,741 + MMJJ_RS02340 0.28 +2.6 442,741 + MMJJ_RS02340 0.28 +1.8 442,741 + MMJJ_RS02340 0.28 -3.0 442,741 + MMJJ_RS02340 0.28 -2.6 442,741 + MMJJ_RS02340 0.28 +2.4 442,741 + MMJJ_RS02340 0.28 +1.0 442,741 + MMJJ_RS02340 0.28 +1.1 442,741 + MMJJ_RS02340 0.28 +1.7 442,765 + MMJJ_RS02340 0.32 +1.5 442,765 + MMJJ_RS02340 0.32 -4.5 442,906 - MMJJ_RS02340 0.58 +0.5 442,906 - MMJJ_RS02340 0.58 -0.4 442,906 - MMJJ_RS02340 0.58 -1.4 442,907 + MMJJ_RS02340 0.58 +2.8 443,078 - MMJJ_RS02340 0.89 +1.4 443,079 + MMJJ_RS02340 0.89 +2.0 443,082 + MMJJ_RS02340 0.90 +1.1 443,082 + MMJJ_RS02340 0.90 -3.3 443,214 + +1.2 443,218 - +1.6 443,236 - +3.5 443,236 - -0.7 443,237 + +0.8 443,238 - +1.1 443,238 - +1.5 443,239 + -0.1 443,240 - -1.9 443,240 - +0.3 443,288 + +2.6 443,301 + -2.7 443,337 - -3.4 443,350 - -1.3 443,354 - +1.7 443,355 + -2.6 443,384 - -2.2 443,384 - -1.9 443,394 + +2.0 443,398 - +1.1 443,402 - -0.2 443,463 - +3.9 443,463 - +1.0 443,463 - +1.2 443,463 - -3.2 443,463 - +0.7 443,464 + -0.8 443,464 + +0.4 443,464 + -0.8 443,464 + +3.6 443,464 + -0.9 443,464 + -2.9 443,465 - -0.9 443,465 - -2.2 443,465 - +2.1 443,465 - +1.6 443,466 + -1.7 443,466 + +2.4 443,467 - -0.9 443,467 - +1.8 443,468 + +1.9 443,468 + -2.2 443,468 + -1.4 443,472 + +1.3 443,490 + +0.2 443,494 + -3.2 443,537 + -0.1 443,538 - -1.9 443,539 + +1.6 443,564 + -2.6 443,583 + +2.3 443,584 - +1.0 443,585 + -2.8 443,742 + MMJJ_RS02345 0.20 -2.3 443,807 - MMJJ_RS02345 0.25 +1.8 443,807 - MMJJ_RS02345 0.25 -1.2 443,808 + MMJJ_RS02345 0.25 -4.4 444,001 + MMJJ_RS02345 0.40 +2.2 444,001 + MMJJ_RS02345 0.40 -1.9 444,007 + MMJJ_RS02345 0.41 +1.6 444,030 - MMJJ_RS02345 0.43 +2.4 444,059 + MMJJ_RS02345 0.45 +1.1 444,059 + MMJJ_RS02345 0.45 +2.1 444,073 - MMJJ_RS02345 0.46 -1.0 444,104 - MMJJ_RS02345 0.48 -2.9 444,105 + MMJJ_RS02345 0.49 +1.1 444,123 - MMJJ_RS02345 0.50 -1.8 444,125 - MMJJ_RS02345 0.50 +1.9 444,126 + MMJJ_RS02345 0.50 -0.6 444,135 - MMJJ_RS02345 0.51 -0.6 444,143 - MMJJ_RS02345 0.51 +2.5 444,209 + MMJJ_RS02345 0.57 +0.9 444,225 - MMJJ_RS02345 0.58 -0.9 444,225 - MMJJ_RS02345 0.58 +2.6 444,238 + MMJJ_RS02345 0.59 +3.6 444,238 + MMJJ_RS02345 0.59 +1.7 444,275 + MMJJ_RS02345 0.62 +2.6 444,275 + MMJJ_RS02345 0.62 +1.5 444,282 - MMJJ_RS02345 0.62 -0.4 444,293 - MMJJ_RS02345 0.63 -0.7 444,317 + MMJJ_RS02345 0.65 +1.2 444,328 - MMJJ_RS02345 0.66 -0.1 444,328 - MMJJ_RS02345 0.66 +0.6 444,329 + MMJJ_RS02345 0.66 +2.4 444,402 - MMJJ_RS02345 0.72 +0.4 444,433 + MMJJ_RS02345 0.74 -0.4 444,490 + MMJJ_RS02345 0.79 -0.8 444,517 - MMJJ_RS02345 0.81 +0.5 444,554 + MMJJ_RS02345 0.84 -3.9 444,682 - +1.7 444,682 - +2.5 444,683 + +1.6 444,697 - +2.8 444,704 + +2.7 444,704 + +1.8 444,719 - +1.4 444,726 - -1.7 444,837 + -3.7 444,873 - -0.2 444,873 - +1.2 444,878 - -0.8 444,899 - -0.2 444,918 + -1.3 444,919 - -2.8 444,920 + -2.5 444,956 + -0.3 444,962 - +1.3 445,021 - -0.3 445,045 + +0.6 445,052 + +2.8 445,064 - -0.4 445,102 - MMJJ_RS02350 0.10 -0.4 445,130 + MMJJ_RS02350 0.13 +0.1 445,153 - MMJJ_RS02350 0.14 +1.0 445,198 - MMJJ_RS02350 0.18 +0.7 445,199 + MMJJ_RS02350 0.18 +1.1 445,203 + MMJJ_RS02350 0.18 +2.0 445,308 + MMJJ_RS02350 0.26 +2.3 445,309 - MMJJ_RS02350 0.26 -3.9 445,309 - MMJJ_RS02350 0.26 +0.1 445,309 - MMJJ_RS02350 0.26 -0.3 445,309 - MMJJ_RS02350 0.26 +2.3 445,309 - MMJJ_RS02350 0.26 +1.3 445,310 + MMJJ_RS02350 0.27 +1.5 445,310 + MMJJ_RS02350 0.27 +0.7 445,310 + MMJJ_RS02350 0.27 -0.7 445,310 + MMJJ_RS02350 0.27 +3.1 445,310 + MMJJ_RS02350 0.27 +0.8 445,314 - MMJJ_RS02350 0.27 +0.7 445,315 + MMJJ_RS02350 0.27 -0.9 445,369 - MMJJ_RS02350 0.31 +1.0 445,370 + MMJJ_RS02350 0.31 -1.1 445,464 + MMJJ_RS02350 0.38 +0.1 445,484 + MMJJ_RS02350 0.40 +0.1 445,595 - MMJJ_RS02350 0.49 -1.2 445,634 - MMJJ_RS02350 0.52 -0.7 445,665 - MMJJ_RS02350 0.54 -2.4 445,665 - MMJJ_RS02350 0.54 -0.7
Or see this region's nucleotide sequence