Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01470

Experiment: McCas_formate_complete Nitrofurantoin 0.025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01465 and MMJJ_RS01470 are separated by 118 nucleotidesMMJJ_RS01470 and MMJJ_RS01475 are separated by 163 nucleotides MMJJ_RS01465: MMJJ_RS01465 - METTL5 family protein, at 286,896 to 287,537 _RS01465 MMJJ_RS01470: MMJJ_RS01470 - Hsp20/alpha crystallin family protein, at 287,656 to 288,120 _RS01470 MMJJ_RS01475: MMJJ_RS01475 - signal recognition particle-docking protein FtsY, at 288,284 to 289,435 _RS01475 Position (kb) 287 288 289Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 286.783 kb on - strandat 286.837 kb on - strandat 286.840 kb on + strandat 286.842 kb on + strandat 286.914 kb on + strandat 286.987 kb on + strand, within MMJJ_RS01465at 286.987 kb on + strand, within MMJJ_RS01465at 286.989 kb on + strand, within MMJJ_RS01465at 286.989 kb on + strandat 287.020 kb on - strand, within MMJJ_RS01465at 287.020 kb on - strand, within MMJJ_RS01465at 287.021 kb on + strand, within MMJJ_RS01465at 287.021 kb on + strand, within MMJJ_RS01465at 287.021 kb on + strand, within MMJJ_RS01465at 287.055 kb on - strand, within MMJJ_RS01465at 287.060 kb on - strand, within MMJJ_RS01465at 287.169 kb on + strand, within MMJJ_RS01465at 287.214 kb on + strand, within MMJJ_RS01465at 287.260 kb on + strand, within MMJJ_RS01465at 287.260 kb on + strand, within MMJJ_RS01465at 287.359 kb on + strand, within MMJJ_RS01465at 287.360 kb on - strand, within MMJJ_RS01465at 287.361 kb on + strand, within MMJJ_RS01465at 287.382 kb on - strand, within MMJJ_RS01465at 287.408 kb on - strand, within MMJJ_RS01465at 287.538 kb on - strandat 287.554 kb on + strandat 287.559 kb on - strandat 287.570 kb on - strandat 287.577 kb on + strandat 287.577 kb on + strandat 287.582 kb on - strandat 287.616 kb on - strandat 287.616 kb on - strandat 287.617 kb on + strandat 287.617 kb on + strandat 287.621 kb on + strandat 287.633 kb on - strandat 287.634 kb on + strandat 287.639 kb on - strandat 287.657 kb on - strandat 287.658 kb on + strandat 287.662 kb on - strandat 287.683 kb on + strandat 287.707 kb on - strand, within MMJJ_RS01470at 287.775 kb on - strand, within MMJJ_RS01470at 287.838 kb on - strand, within MMJJ_RS01470at 287.894 kb on + strand, within MMJJ_RS01470at 287.924 kb on + strand, within MMJJ_RS01470at 287.968 kb on + strand, within MMJJ_RS01470at 287.988 kb on - strand, within MMJJ_RS01470at 287.989 kb on + strand, within MMJJ_RS01470at 288.055 kb on + strand, within MMJJ_RS01470at 288.201 kb on - strandat 288.202 kb on + strandat 288.202 kb on + strandat 288.202 kb on + strandat 288.206 kb on - strandat 288.210 kb on - strandat 288.216 kb on - strandat 288.217 kb on + strandat 288.219 kb on - strandat 288.219 kb on - strandat 288.219 kb on - strandat 288.220 kb on + strandat 288.220 kb on + strandat 288.221 kb on - strandat 288.221 kb on - strandat 288.221 kb on - strandat 288.222 kb on + strandat 288.222 kb on + strandat 288.222 kb on + strandat 288.226 kb on + strandat 288.238 kb on - strandat 288.238 kb on - strandat 288.238 kb on - strandat 288.238 kb on - strandat 288.238 kb on - strandat 288.240 kb on - strandat 288.241 kb on + strandat 288.747 kb on - strand, within MMJJ_RS01475at 288.835 kb on + strandat 288.862 kb on + strand, within MMJJ_RS01475at 289.079 kb on + strand, within MMJJ_RS01475at 289.096 kb on - strand, within MMJJ_RS01475

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete Nitrofurantoin 0.025 mM
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286,783 - -0.3
286,837 - +1.3
286,840 + -0.3
286,842 + -1.8
286,914 + +4.1
286,987 + MMJJ_RS01465 0.14 -2.5
286,987 + MMJJ_RS01465 0.14 -2.1
286,989 + MMJJ_RS01465 0.14 +1.5
286,989 + +2.3
287,020 - MMJJ_RS01465 0.19 -1.4
287,020 - MMJJ_RS01465 0.19 -2.0
287,021 + MMJJ_RS01465 0.19 -1.2
287,021 + MMJJ_RS01465 0.19 -1.2
287,021 + MMJJ_RS01465 0.19 -1.3
287,055 - MMJJ_RS01465 0.25 -1.9
287,060 - MMJJ_RS01465 0.26 -2.3
287,169 + MMJJ_RS01465 0.43 -2.6
287,214 + MMJJ_RS01465 0.50 -2.8
287,260 + MMJJ_RS01465 0.57 +1.0
287,260 + MMJJ_RS01465 0.57 -3.3
287,359 + MMJJ_RS01465 0.72 -0.4
287,360 - MMJJ_RS01465 0.72 +2.1
287,361 + MMJJ_RS01465 0.72 +0.5
287,382 - MMJJ_RS01465 0.76 +2.4
287,408 - MMJJ_RS01465 0.80 +0.3
287,538 - -3.0
287,554 + +1.3
287,559 - +3.4
287,570 - +3.1
287,577 + -1.0
287,577 + -1.3
287,582 - +3.6
287,616 - -3.1
287,616 - -2.3
287,617 + +0.1
287,617 + +1.3
287,621 + +0.3
287,633 - +1.0
287,634 + +2.4
287,639 - +1.0
287,657 - +0.7
287,658 + +3.7
287,662 - -1.7
287,683 + -2.5
287,707 - MMJJ_RS01470 0.11 -1.3
287,775 - MMJJ_RS01470 0.26 -2.1
287,838 - MMJJ_RS01470 0.39 +0.2
287,894 + MMJJ_RS01470 0.51 +1.1
287,924 + MMJJ_RS01470 0.58 -1.9
287,968 + MMJJ_RS01470 0.67 +1.6
287,988 - MMJJ_RS01470 0.71 -2.0
287,989 + MMJJ_RS01470 0.72 -2.4
288,055 + MMJJ_RS01470 0.86 -0.3
288,201 - +1.7
288,202 + +2.2
288,202 + -4.0
288,202 + +2.1
288,206 - +3.4
288,210 - -2.4
288,216 - -1.1
288,217 + -1.0
288,219 - -2.9
288,219 - -0.5
288,219 - +1.4
288,220 + +2.5
288,220 + +1.9
288,221 - -3.1
288,221 - +0.6
288,221 - -0.3
288,222 + -3.1
288,222 + +1.3
288,222 + +0.2
288,226 + +1.6
288,238 - +1.4
288,238 - +1.1
288,238 - -3.8
288,238 - +0.5
288,238 - +2.9
288,240 - +3.3
288,241 + +1.0
288,747 - MMJJ_RS01475 0.40 -2.4
288,835 + +0.3
288,862 + MMJJ_RS01475 0.50 +0.3
289,079 + MMJJ_RS01475 0.69 +0.3
289,096 - MMJJ_RS01475 0.70 +2.3

Or see this region's nucleotide sequence