Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01845 and MMJJ_RS09265 are separated by 80 nucleotides MMJJ_RS09265 and MMJJ_RS01850 are separated by 8 nucleotides MMJJ_RS01850 and MMJJ_RS01855 are separated by 1 nucleotides MMJJ_RS01855 and MMJJ_RS01860 are separated by 53 nucleotides
MMJJ_RS01845: MMJJ_RS01845 - acetolactate decarboxylase, at 354,045 to 354,845
_RS01845
MMJJ_RS09265: MMJJ_RS09265 - hypothetical protein, at 354,926 to 355,072
_RS09265
MMJJ_RS01850: MMJJ_RS01850 - RNA 2',3'-cyclic phosphodiesterase, at 355,081 to 355,605
_RS01850
MMJJ_RS01855: MMJJ_RS01855 - MBL fold metallo-hydrolase, at 355,607 to 356,149
_RS01855
MMJJ_RS01860: MMJJ_RS01860 - tRNA guanosine(15) transglycosylase TgtA, at 356,203 to 358,152
_RS01860
Position (kb) 355
356
357 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 354.640 kb on + strand, within MMJJ_RS01845 at 354.679 kb on + strand, within MMJJ_RS01845 at 354.691 kb on - strand, within MMJJ_RS01845 at 354.791 kb on - strand at 354.791 kb on - strand at 354.791 kb on - strand at 354.804 kb on - strand at 354.813 kb on - strand at 354.813 kb on - strand at 354.816 kb on + strand at 354.819 kb on - strand at 354.840 kb on + strand at 354.846 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.877 kb on + strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.878 kb on - strand at 354.879 kb on + strand at 354.879 kb on + strand at 354.882 kb on - strand at 354.883 kb on + strand at 354.883 kb on + strand at 354.892 kb on - strand at 354.896 kb on - strand at 354.897 kb on + strand at 354.900 kb on + strand at 354.915 kb on + strand at 354.920 kb on - strand at 354.921 kb on + strand at 355.006 kb on + strand, within MMJJ_RS09265 at 355.039 kb on - strand, within MMJJ_RS09265 at 355.098 kb on - strand at 355.119 kb on + strand at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.318 kb on - strand, within MMJJ_RS01850 at 355.319 kb on + strand, within MMJJ_RS01850 at 355.366 kb on + strand, within MMJJ_RS01850 at 355.447 kb on + strand, within MMJJ_RS01850 at 355.487 kb on - strand, within MMJJ_RS01850 at 355.507 kb on + strand, within MMJJ_RS01850 at 355.529 kb on - strand, within MMJJ_RS01850 at 355.530 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.546 kb on + strand, within MMJJ_RS01850 at 355.554 kb on - strand at 355.564 kb on + strand at 355.579 kb on - strand at 355.614 kb on - strand at 355.627 kb on + strand at 355.651 kb on - strand at 355.651 kb on - strand at 355.652 kb on + strand at 355.673 kb on - strand, within MMJJ_RS01855 at 355.723 kb on - strand, within MMJJ_RS01855 at 355.724 kb on + strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.779 kb on - strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.780 kb on + strand, within MMJJ_RS01855 at 355.801 kb on - strand, within MMJJ_RS01855 at 355.853 kb on + strand, within MMJJ_RS01855 at 355.862 kb on - strand, within MMJJ_RS01855 at 355.879 kb on + strand, within MMJJ_RS01855 at 355.882 kb on - strand, within MMJJ_RS01855 at 355.901 kb on + strand, within MMJJ_RS01855 at 355.947 kb on + strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.948 kb on - strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.949 kb on + strand, within MMJJ_RS01855 at 355.951 kb on - strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.952 kb on + strand, within MMJJ_RS01855 at 355.953 kb on - strand, within MMJJ_RS01855 at 355.954 kb on + strand, within MMJJ_RS01855 at 355.979 kb on - strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 355.980 kb on + strand, within MMJJ_RS01855 at 356.030 kb on + strand, within MMJJ_RS01855 at 356.039 kb on - strand, within MMJJ_RS01855 at 356.060 kb on - strand, within MMJJ_RS01855 at 356.061 kb on + strand, within MMJJ_RS01855 at 356.063 kb on + strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.064 kb on - strand, within MMJJ_RS01855 at 356.068 kb on - strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.069 kb on + strand, within MMJJ_RS01855 at 356.094 kb on + strand, within MMJJ_RS01855 at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.133 kb on - strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.134 kb on + strand at 356.144 kb on - strand at 356.175 kb on + strand at 356.191 kb on + strand at 356.195 kb on - strand at 356.198 kb on - strand at 356.199 kb on + strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.275 kb on - strand at 356.284 kb on + strand at 356.289 kb on + strand at 356.296 kb on - strand at 356.423 kb on + strand, within MMJJ_RS01860 at 356.426 kb on + strand, within MMJJ_RS01860 at 356.432 kb on - strand, within MMJJ_RS01860 at 356.458 kb on - strand, within MMJJ_RS01860 at 356.514 kb on - strand, within MMJJ_RS01860 at 356.515 kb on + strand, within MMJJ_RS01860 at 356.621 kb on - strand, within MMJJ_RS01860 at 356.701 kb on - strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.702 kb on + strand, within MMJJ_RS01860 at 356.705 kb on - strand, within MMJJ_RS01860 at 356.706 kb on + strand, within MMJJ_RS01860 at 356.706 kb on + strand, within MMJJ_RS01860 at 356.706 kb on + strand at 356.706 kb on + strand, within MMJJ_RS01860 at 356.708 kb on - strand at 356.708 kb on - strand, within MMJJ_RS01860 at 356.709 kb on + strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.714 kb on - strand, within MMJJ_RS01860 at 356.715 kb on + strand, within MMJJ_RS01860 at 356.718 kb on - strand, within MMJJ_RS01860 at 356.718 kb on - strand, within MMJJ_RS01860 at 356.722 kb on - strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.723 kb on + strand, within MMJJ_RS01860 at 356.778 kb on - strand, within MMJJ_RS01860 at 356.885 kb on + strand, within MMJJ_RS01860 at 356.902 kb on - strand at 356.902 kb on - strand, within MMJJ_RS01860 at 356.902 kb on - strand, within MMJJ_RS01860 at 356.902 kb on - strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.903 kb on + strand, within MMJJ_RS01860 at 356.937 kb on - strand, within MMJJ_RS01860 at 356.956 kb on - strand, within MMJJ_RS01860 at 356.972 kb on - strand, within MMJJ_RS01860 at 357.005 kb on - strand, within MMJJ_RS01860 at 357.018 kb on - strand, within MMJJ_RS01860 at 357.024 kb on - strand, within MMJJ_RS01860 at 357.093 kb on - strand, within MMJJ_RS01860 at 357.095 kb on - strand, within MMJJ_RS01860 at 357.096 kb on + strand, within MMJJ_RS01860
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 354,640 + MMJJ_RS01845 0.74 -1.8 354,679 + MMJJ_RS01845 0.79 +0.5 354,691 - MMJJ_RS01845 0.81 -0.5 354,791 - +3.3 354,791 - +2.2 354,791 - -2.0 354,804 - +2.1 354,813 - -0.3 354,813 - -1.0 354,816 + -1.5 354,819 - +1.8 354,840 + +0.8 354,846 + -1.0 354,877 + -1.1 354,877 + -1.6 354,877 + -0.4 354,878 - +0.9 354,878 - +0.8 354,878 - +1.0 354,879 + +0.7 354,879 + +1.5 354,882 - -1.5 354,883 + -0.7 354,883 + -1.9 354,892 - -2.0 354,896 - +0.2 354,897 + -3.2 354,900 + +0.9 354,915 + +1.5 354,920 - +1.1 354,921 + +1.3 355,006 + MMJJ_RS09265 0.54 -3.1 355,039 - MMJJ_RS09265 0.77 -0.8 355,098 - +0.7 355,119 + +1.1 355,318 - MMJJ_RS01850 0.45 -0.5 355,318 - MMJJ_RS01850 0.45 -1.2 355,318 - MMJJ_RS01850 0.45 +1.3 355,319 + MMJJ_RS01850 0.45 +0.7 355,366 + MMJJ_RS01850 0.54 -0.0 355,447 + MMJJ_RS01850 0.70 +0.1 355,487 - MMJJ_RS01850 0.77 -0.4 355,507 + MMJJ_RS01850 0.81 -0.9 355,529 - MMJJ_RS01850 0.85 +3.7 355,530 + MMJJ_RS01850 0.86 +2.3 355,546 + MMJJ_RS01850 0.89 -3.7 355,546 + MMJJ_RS01850 0.89 -0.4 355,554 - +0.6 355,564 + +0.2 355,579 - +0.8 355,614 - -0.9 355,627 + +1.1 355,651 - -0.2 355,651 - +0.9 355,652 + +2.2 355,673 - MMJJ_RS01855 0.12 +0.1 355,723 - MMJJ_RS01855 0.21 +1.2 355,724 + MMJJ_RS01855 0.22 -1.1 355,779 - MMJJ_RS01855 0.32 -1.7 355,779 - MMJJ_RS01855 0.32 -3.2 355,779 - MMJJ_RS01855 0.32 -0.2 355,779 - MMJJ_RS01855 0.32 +1.0 355,779 - MMJJ_RS01855 0.32 +1.1 355,780 + MMJJ_RS01855 0.32 -3.3 355,780 + MMJJ_RS01855 0.32 -2.0 355,801 - MMJJ_RS01855 0.36 +2.1 355,853 + MMJJ_RS01855 0.45 +0.6 355,862 - MMJJ_RS01855 0.47 +1.8 355,879 + MMJJ_RS01855 0.50 -1.1 355,882 - MMJJ_RS01855 0.51 -3.3 355,901 + MMJJ_RS01855 0.54 -1.9 355,947 + MMJJ_RS01855 0.63 +0.6 355,948 - MMJJ_RS01855 0.63 -3.8 355,948 - MMJJ_RS01855 0.63 +2.3 355,948 - MMJJ_RS01855 0.63 +1.5 355,949 + MMJJ_RS01855 0.63 -2.5 355,949 + MMJJ_RS01855 0.63 +2.7 355,951 - MMJJ_RS01855 0.63 +2.8 355,952 + MMJJ_RS01855 0.64 -1.4 355,952 + MMJJ_RS01855 0.64 +3.1 355,952 + MMJJ_RS01855 0.64 +0.2 355,953 - MMJJ_RS01855 0.64 +2.6 355,954 + MMJJ_RS01855 0.64 +5.6 355,979 - MMJJ_RS01855 0.69 +4.2 355,980 + MMJJ_RS01855 0.69 +1.1 355,980 + MMJJ_RS01855 0.69 +0.7 356,030 + MMJJ_RS01855 0.78 -3.1 356,039 - MMJJ_RS01855 0.80 -2.2 356,060 - MMJJ_RS01855 0.83 +3.1 356,061 + MMJJ_RS01855 0.84 +2.2 356,063 + MMJJ_RS01855 0.84 +1.0 356,064 - MMJJ_RS01855 0.84 +2.1 356,064 - MMJJ_RS01855 0.84 +4.8 356,068 - MMJJ_RS01855 0.85 +1.7 356,069 + MMJJ_RS01855 0.85 -0.8 356,069 + MMJJ_RS01855 0.85 +0.1 356,069 + MMJJ_RS01855 0.85 -2.6 356,069 + MMJJ_RS01855 0.85 -2.5 356,094 + MMJJ_RS01855 0.90 +3.8 356,133 - -1.2 356,133 - +0.3 356,133 - -1.1 356,133 - +0.8 356,133 - +0.9 356,133 - -0.9 356,133 - +3.9 356,134 + -2.2 356,134 + +0.8 356,134 + +0.1 356,134 + -1.4 356,134 + +0.2 356,134 + +1.3 356,134 + +1.0 356,134 + -1.2 356,134 + +1.7 356,144 - -2.3 356,175 + -3.3 356,191 + +1.4 356,195 - -1.4 356,198 - +1.3 356,199 + +3.4 356,275 - -0.4 356,275 - +0.1 356,275 - +1.4 356,275 - +0.8 356,284 + +2.8 356,289 + +1.1 356,296 - -1.2 356,423 + MMJJ_RS01860 0.11 -1.9 356,426 + MMJJ_RS01860 0.11 -0.9 356,432 - MMJJ_RS01860 0.12 +2.5 356,458 - MMJJ_RS01860 0.13 -3.4 356,514 - MMJJ_RS01860 0.16 -2.2 356,515 + MMJJ_RS01860 0.16 +3.1 356,621 - MMJJ_RS01860 0.21 -3.4 356,701 - MMJJ_RS01860 0.26 -0.4 356,702 + MMJJ_RS01860 0.26 -0.9 356,702 + MMJJ_RS01860 0.26 -2.0 356,702 + MMJJ_RS01860 0.26 -1.3 356,702 + MMJJ_RS01860 0.26 -1.4 356,705 - MMJJ_RS01860 0.26 +0.1 356,706 + MMJJ_RS01860 0.26 -1.5 356,706 + MMJJ_RS01860 0.26 +0.1 356,706 + -0.4 356,706 + MMJJ_RS01860 0.26 -1.4 356,708 - +4.3 356,708 - MMJJ_RS01860 0.26 -0.9 356,709 + MMJJ_RS01860 0.26 -1.2 356,714 - MMJJ_RS01860 0.26 -2.3 356,714 - MMJJ_RS01860 0.26 +4.8 356,714 - MMJJ_RS01860 0.26 -1.4 356,715 + MMJJ_RS01860 0.26 -3.0 356,718 - MMJJ_RS01860 0.26 -1.4 356,718 - MMJJ_RS01860 0.26 +0.7 356,722 - MMJJ_RS01860 0.27 -0.7 356,723 + MMJJ_RS01860 0.27 -0.9 356,723 + MMJJ_RS01860 0.27 +0.1 356,723 + MMJJ_RS01860 0.27 -1.2 356,778 - MMJJ_RS01860 0.29 -1.2 356,885 + MMJJ_RS01860 0.35 +1.1 356,902 - -0.4 356,902 - MMJJ_RS01860 0.36 +1.1 356,902 - MMJJ_RS01860 0.36 -1.6 356,902 - MMJJ_RS01860 0.36 -2.7 356,903 + MMJJ_RS01860 0.36 +1.3 356,903 + MMJJ_RS01860 0.36 +4.3 356,903 + MMJJ_RS01860 0.36 -1.4 356,937 - MMJJ_RS01860 0.38 -2.9 356,956 - MMJJ_RS01860 0.39 -3.2 356,972 - MMJJ_RS01860 0.39 +0.0 357,005 - MMJJ_RS01860 0.41 +0.1 357,018 - MMJJ_RS01860 0.42 +0.1 357,024 - MMJJ_RS01860 0.42 -1.7 357,093 - MMJJ_RS01860 0.46 -2.6 357,095 - MMJJ_RS01860 0.46 -2.3 357,096 + MMJJ_RS01860 0.46 -0.8
Or see this region's nucleotide sequence