Experiment: McCas_formate_complete Antimycin A 0.01 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00130 and MMJJ_RS00135 are separated by 86 nucleotides MMJJ_RS00135 and MMJJ_RS00140 are separated by 48 nucleotides MMJJ_RS00140 and MMJJ_RS00145 are separated by 125 nucleotides
MMJJ_RS00130: MMJJ_RS00130 - uroporphyrinogen-III C-methyltransferase, at 24,902 to 25,621
_RS00130
MMJJ_RS00135: MMJJ_RS00135 - FmdE family protein, at 25,708 to 26,286
_RS00135
MMJJ_RS00140: MMJJ_RS00140 - hypothetical protein, at 26,335 to 26,592
_RS00140
MMJJ_RS00145: MMJJ_RS00145 - aquaporin family protein, at 26,718 to 27,437
_RS00145
Position (kb) 25
26
27 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 24.714 kb on + strand at 24.725 kb on - strand at 24.726 kb on + strand at 24.743 kb on - strand at 24.744 kb on + strand at 24.745 kb on - strand at 24.745 kb on - strand at 24.749 kb on - strand at 24.753 kb on - strand at 24.754 kb on + strand at 24.779 kb on + strand at 24.796 kb on + strand at 24.817 kb on + strand at 24.829 kb on + strand at 24.833 kb on + strand at 24.855 kb on - strand at 24.855 kb on - strand at 24.856 kb on + strand at 24.856 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 24.861 kb on + strand at 25.147 kb on + strand, within MMJJ_RS00130 at 25.221 kb on - strand, within MMJJ_RS00130 at 25.246 kb on - strand, within MMJJ_RS00130 at 25.615 kb on + strand at 25.652 kb on - strand at 25.653 kb on + strand at 25.653 kb on + strand at 25.653 kb on + strand at 25.673 kb on - strand at 25.673 kb on - strand at 25.685 kb on - strand at 25.686 kb on + strand at 25.789 kb on - strand, within MMJJ_RS00135 at 25.789 kb on - strand, within MMJJ_RS00135 at 25.796 kb on - strand, within MMJJ_RS00135 at 25.804 kb on - strand, within MMJJ_RS00135 at 25.804 kb on - strand, within MMJJ_RS00135 at 25.805 kb on + strand, within MMJJ_RS00135 at 25.849 kb on + strand, within MMJJ_RS00135 at 25.860 kb on - strand, within MMJJ_RS00135 at 25.860 kb on - strand, within MMJJ_RS00135 at 25.871 kb on - strand, within MMJJ_RS00135 at 25.939 kb on - strand, within MMJJ_RS00135 at 25.991 kb on + strand, within MMJJ_RS00135 at 26.009 kb on - strand, within MMJJ_RS00135 at 26.009 kb on - strand, within MMJJ_RS00135 at 26.136 kb on + strand, within MMJJ_RS00135 at 26.160 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.187 kb on - strand, within MMJJ_RS00135 at 26.188 kb on + strand, within MMJJ_RS00135 at 26.215 kb on + strand, within MMJJ_RS00135 at 26.235 kb on - strand at 26.235 kb on - strand at 26.249 kb on - strand at 26.250 kb on + strand at 26.257 kb on + strand at 26.289 kb on + strand at 26.353 kb on - strand at 26.353 kb on - strand at 26.357 kb on - strand at 26.357 kb on - strand at 26.357 kb on - strand at 26.358 kb on + strand at 26.361 kb on - strand, within MMJJ_RS00140 at 26.398 kb on - strand, within MMJJ_RS00140 at 26.399 kb on + strand, within MMJJ_RS00140 at 26.399 kb on + strand, within MMJJ_RS00140 at 26.400 kb on - strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.401 kb on + strand, within MMJJ_RS00140 at 26.402 kb on - strand, within MMJJ_RS00140 at 26.403 kb on + strand, within MMJJ_RS00140 at 26.407 kb on - strand, within MMJJ_RS00140 at 26.408 kb on + strand, within MMJJ_RS00140 at 26.412 kb on - strand, within MMJJ_RS00140 at 26.574 kb on + strand at 26.583 kb on - strand at 26.604 kb on - strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.609 kb on + strand at 26.611 kb on - strand at 26.614 kb on - strand at 26.614 kb on - strand at 26.615 kb on + strand at 26.619 kb on + strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.730 kb on - strand at 26.740 kb on - strand at 26.740 kb on - strand at 26.741 kb on + strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.744 kb on - strand at 26.745 kb on + strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.757 kb on - strand at 26.758 kb on + strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.783 kb on - strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.784 kb on + strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.786 kb on + strand at 26.788 kb on + strand at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.797 kb on - strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.801 kb on + strand, within MMJJ_RS00145 at 26.807 kb on - strand, within MMJJ_RS00145 at 26.822 kb on - strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.823 kb on + strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.825 kb on - strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.826 kb on + strand, within MMJJ_RS00145 at 26.829 kb on - strand, within MMJJ_RS00145 at 26.830 kb on + strand, within MMJJ_RS00145 at 26.863 kb on + strand, within MMJJ_RS00145 at 26.880 kb on + strand, within MMJJ_RS00145 at 26.973 kb on - strand, within MMJJ_RS00145 at 26.975 kb on - strand, within MMJJ_RS00145 at 27.003 kb on + strand, within MMJJ_RS00145 at 27.075 kb on - strand, within MMJJ_RS00145 at 27.108 kb on - strand, within MMJJ_RS00145 at 27.133 kb on + strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.146 kb on - strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.147 kb on + strand, within MMJJ_RS00145 at 27.148 kb on - strand, within MMJJ_RS00145 at 27.149 kb on + strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.150 kb on - strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.151 kb on + strand, within MMJJ_RS00145 at 27.159 kb on + strand, within MMJJ_RS00145 at 27.200 kb on - strand, within MMJJ_RS00145 at 27.201 kb on + strand, within MMJJ_RS00145 at 27.213 kb on + strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.230 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.242 kb on - strand, within MMJJ_RS00145 at 27.249 kb on - strand, within MMJJ_RS00145 at 27.250 kb on + strand, within MMJJ_RS00145
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Antimycin A 0.01 mM remove 24,714 + -0.9 24,725 - -0.7 24,726 + -0.9 24,743 - +1.1 24,744 + -1.9 24,745 - -1.7 24,745 - -1.3 24,749 - -1.3 24,753 - +3.3 24,754 + +0.3 24,779 + -0.6 24,796 + -2.1 24,817 + -1.9 24,829 + -1.6 24,833 + -0.5 24,855 - -2.4 24,855 - -1.7 24,856 + -2.5 24,856 + -1.5 24,861 + -0.9 24,861 + -1.9 24,861 + -0.9 25,147 + MMJJ_RS00130 0.34 +0.1 25,221 - MMJJ_RS00130 0.44 -0.5 25,246 - MMJJ_RS00130 0.48 -0.7 25,615 + +0.9 25,652 - +1.4 25,653 + -0.6 25,653 + +1.9 25,653 + +0.9 25,673 - +1.0 25,673 - -2.3 25,685 - +0.8 25,686 + +2.6 25,789 - MMJJ_RS00135 0.14 +1.1 25,789 - MMJJ_RS00135 0.14 +3.8 25,796 - MMJJ_RS00135 0.15 -1.9 25,804 - MMJJ_RS00135 0.17 +2.2 25,804 - MMJJ_RS00135 0.17 -0.1 25,805 + MMJJ_RS00135 0.17 +0.8 25,849 + MMJJ_RS00135 0.24 +4.4 25,860 - MMJJ_RS00135 0.26 +0.8 25,860 - MMJJ_RS00135 0.26 -1.7 25,871 - MMJJ_RS00135 0.28 -0.9 25,939 - MMJJ_RS00135 0.40 +0.3 25,991 + MMJJ_RS00135 0.49 +3.4 26,009 - MMJJ_RS00135 0.52 +1.3 26,009 - MMJJ_RS00135 0.52 +0.1 26,136 + MMJJ_RS00135 0.74 +3.9 26,160 - MMJJ_RS00135 0.78 -0.3 26,187 - MMJJ_RS00135 0.83 -2.1 26,187 - MMJJ_RS00135 0.83 -0.8 26,187 - MMJJ_RS00135 0.83 +0.1 26,187 - MMJJ_RS00135 0.83 +0.4 26,188 + MMJJ_RS00135 0.83 -1.4 26,215 + MMJJ_RS00135 0.88 +2.7 26,235 - +1.9 26,235 - +2.8 26,249 - +2.9 26,250 + +0.4 26,257 + +0.5 26,289 + -0.4 26,353 - -0.0 26,353 - +1.1 26,357 - -2.7 26,357 - +0.2 26,357 - +3.1 26,358 + +1.1 26,361 - MMJJ_RS00140 0.10 +1.1 26,398 - MMJJ_RS00140 0.24 -0.9 26,399 + MMJJ_RS00140 0.25 +2.8 26,399 + MMJJ_RS00140 0.25 +0.5 26,400 - MMJJ_RS00140 0.25 -2.6 26,401 + MMJJ_RS00140 0.26 -2.9 26,401 + MMJJ_RS00140 0.26 -0.9 26,401 + MMJJ_RS00140 0.26 -2.0 26,402 - MMJJ_RS00140 0.26 +3.2 26,403 + MMJJ_RS00140 0.26 -0.3 26,407 - MMJJ_RS00140 0.28 -1.3 26,408 + MMJJ_RS00140 0.28 +0.6 26,412 - MMJJ_RS00140 0.30 +3.0 26,574 + -2.5 26,583 - -0.9 26,604 - +1.8 26,609 + +3.5 26,609 + +0.6 26,609 + +0.8 26,611 - -2.8 26,614 - +0.6 26,614 - +1.8 26,615 + +1.1 26,619 + +1.6 26,730 - -0.7 26,730 - -0.4 26,730 - -0.7 26,740 - -1.7 26,740 - -0.9 26,741 + +2.1 26,744 - -4.5 26,744 - -0.5 26,744 - +3.5 26,745 + -2.9 26,757 - -0.7 26,757 - -3.2 26,757 - -3.5 26,757 - +1.5 26,758 + -0.7 26,783 - -0.5 26,783 - -3.1 26,783 - +0.2 26,783 - -4.1 26,784 + +0.3 26,784 + -0.6 26,784 + +0.7 26,785 - +1.3 26,785 - +0.9 26,785 - -1.3 26,785 - +2.1 26,785 - +2.9 26,785 - +0.2 26,786 + -1.3 26,786 + -2.1 26,786 + -0.9 26,786 + +2.7 26,786 + -3.1 26,786 + -2.9 26,786 + -2.5 26,788 + +2.7 26,797 - MMJJ_RS00145 0.11 +2.1 26,797 - MMJJ_RS00145 0.11 +1.0 26,797 - MMJJ_RS00145 0.11 -0.4 26,801 + MMJJ_RS00145 0.12 +1.5 26,801 + MMJJ_RS00145 0.12 +1.8 26,807 - MMJJ_RS00145 0.12 -2.9 26,822 - MMJJ_RS00145 0.14 +0.6 26,823 + MMJJ_RS00145 0.15 -0.5 26,823 + MMJJ_RS00145 0.15 -1.2 26,825 - MMJJ_RS00145 0.15 -0.9 26,825 - MMJJ_RS00145 0.15 -2.1 26,825 - MMJJ_RS00145 0.15 -3.0 26,826 + MMJJ_RS00145 0.15 +0.6 26,826 + MMJJ_RS00145 0.15 +2.1 26,826 + MMJJ_RS00145 0.15 +0.2 26,826 + MMJJ_RS00145 0.15 +1.4 26,826 + MMJJ_RS00145 0.15 +0.0 26,829 - MMJJ_RS00145 0.15 -3.7 26,830 + MMJJ_RS00145 0.16 +1.2 26,863 + MMJJ_RS00145 0.20 +0.7 26,880 + MMJJ_RS00145 0.23 +1.9 26,973 - MMJJ_RS00145 0.35 -2.8 26,975 - MMJJ_RS00145 0.36 -1.6 27,003 + MMJJ_RS00145 0.40 -2.4 27,075 - MMJJ_RS00145 0.50 -2.9 27,108 - MMJJ_RS00145 0.54 +1.5 27,133 + MMJJ_RS00145 0.58 -0.9 27,146 - MMJJ_RS00145 0.59 -3.1 27,146 - MMJJ_RS00145 0.59 +1.2 27,147 + MMJJ_RS00145 0.60 -2.9 27,147 + MMJJ_RS00145 0.60 -1.0 27,148 - MMJJ_RS00145 0.60 +2.9 27,149 + MMJJ_RS00145 0.60 -0.7 27,150 - MMJJ_RS00145 0.60 +0.1 27,150 - MMJJ_RS00145 0.60 -2.1 27,150 - MMJJ_RS00145 0.60 -2.7 27,150 - MMJJ_RS00145 0.60 +0.1 27,150 - MMJJ_RS00145 0.60 +0.7 27,151 + MMJJ_RS00145 0.60 +0.1 27,151 + MMJJ_RS00145 0.60 -0.9 27,151 + MMJJ_RS00145 0.60 +3.6 27,151 + MMJJ_RS00145 0.60 -2.0 27,159 + MMJJ_RS00145 0.61 +2.6 27,200 - MMJJ_RS00145 0.67 -2.6 27,201 + MMJJ_RS00145 0.67 -0.7 27,213 + MMJJ_RS00145 0.69 -3.3 27,230 - MMJJ_RS00145 0.71 -2.4 27,230 - MMJJ_RS00145 0.71 +0.8 27,242 - MMJJ_RS00145 0.73 -1.6 27,242 - MMJJ_RS00145 0.73 -0.9 27,242 - MMJJ_RS00145 0.73 +1.5 27,249 - MMJJ_RS00145 0.74 +1.9 27,250 + MMJJ_RS00145 0.74 +0.1
Or see this region's nucleotide sequence