Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20330 and PGA1_c20340 are separated by 201 nucleotides PGA1_c20340 and PGA1_c20350 are separated by 131 nucleotides
PGA1_c20330: PGA1_c20330 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase IspG, at 2,112,455 to 2,113,576
_c20330
PGA1_c20340: PGA1_c20340 - Uncharacterized protein conserved in bacteria, at 2,113,778 to 2,115,001
_c20340
PGA1_c20350: PGA1_c20350 - 5-aminolevulinate synthase HemA, at 2,115,133 to 2,116,362
_c20350
Position (kb)
2113
2114
2115
2116 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2113.634 kb on + strand at 2113.668 kb on + strand at 2113.676 kb on - strand at 2113.724 kb on + strand at 2113.743 kb on - strand at 2113.975 kb on + strand, within PGA1_c20340 at 2114.002 kb on - strand, within PGA1_c20340 at 2114.002 kb on - strand, within PGA1_c20340 at 2114.002 kb on - strand, within PGA1_c20340 at 2114.002 kb on - strand, within PGA1_c20340 at 2114.002 kb on - strand, within PGA1_c20340 at 2114.009 kb on - strand, within PGA1_c20340 at 2114.140 kb on - strand, within PGA1_c20340 at 2115.024 kb on + strand at 2115.106 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 2,113,634 + +1.0 2,113,668 + +1.7 2,113,676 - +0.2 2,113,724 + +0.0 2,113,743 - +1.4 2,113,975 + PGA1_c20340 0.16 -0.4 2,114,002 - PGA1_c20340 0.18 -0.4 2,114,002 - PGA1_c20340 0.18 -0.8 2,114,002 - PGA1_c20340 0.18 -1.2 2,114,002 - PGA1_c20340 0.18 -2.4 2,114,002 - PGA1_c20340 0.18 -2.9 2,114,009 - PGA1_c20340 0.19 -0.6 2,114,140 - PGA1_c20340 0.30 -2.5 2,115,024 + -1.0 2,115,106 - -1.0 2,115,196 - +0.4 2,115,196 - +1.4 2,115,196 - -0.1 2,115,196 - -0.1 2,115,196 - +1.0
Or see this region's nucleotide sequence