Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5060 and PP_5061 are separated by 87 nucleotides PP_5061 and PP_5719 are separated by 475 nucleotides
PP_5060: PP_5060 - conserved protein of unknown function, at 5,766,156 to 5,770,871
_5060
PP_5061: PP_5061 - Choline transporter, at 5,770,959 to 5,772,962
_5061
PP_5719: PP_5719 - choline-responsive transcriptional repressor BetI, at 5,773,438 to 5,774,094
_5719
Position (kb) 5770
5771
5772
5773 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5770.006 kb on + strand, within PP_5060 at 5770.083 kb on + strand, within PP_5060 at 5770.084 kb on - strand, within PP_5060 at 5770.084 kb on - strand, within PP_5060 at 5770.233 kb on + strand, within PP_5060 at 5770.233 kb on + strand, within PP_5060 at 5770.233 kb on + strand, within PP_5060 at 5770.234 kb on - strand, within PP_5060 at 5770.234 kb on - strand, within PP_5060 at 5770.234 kb on - strand, within PP_5060 at 5770.234 kb on - strand, within PP_5060 at 5770.409 kb on - strand at 5770.675 kb on + strand at 5770.676 kb on - strand at 5770.726 kb on + strand at 5770.761 kb on + strand at 5770.903 kb on + strand at 5770.903 kb on + strand at 5770.903 kb on + strand at 5770.903 kb on + strand at 5770.903 kb on + strand at 5770.904 kb on - strand at 5770.904 kb on - strand at 5770.904 kb on - strand at 5770.904 kb on - strand at 5770.904 kb on - strand at 5770.907 kb on + strand at 5770.907 kb on + strand at 5770.907 kb on + strand at 5770.907 kb on + strand at 5770.907 kb on + strand at 5770.908 kb on - strand at 5770.908 kb on - strand at 5770.964 kb on + strand at 5770.964 kb on + strand at 5770.964 kb on + strand at 5770.965 kb on - strand at 5770.966 kb on + strand at 5770.966 kb on + strand at 5770.967 kb on - strand at 5771.053 kb on + strand at 5771.054 kb on - strand at 5771.054 kb on - strand at 5771.077 kb on + strand at 5771.077 kb on + strand at 5771.078 kb on - strand at 5771.151 kb on - strand at 5771.151 kb on - strand at 5771.152 kb on + strand at 5771.152 kb on + strand at 5771.152 kb on + strand at 5771.152 kb on + strand at 5771.152 kb on + strand at 5771.153 kb on - strand at 5771.153 kb on - strand at 5771.153 kb on - strand at 5771.174 kb on - strand, within PP_5061 at 5771.446 kb on + strand, within PP_5061 at 5771.447 kb on - strand, within PP_5061 at 5771.447 kb on - strand, within PP_5061 at 5771.918 kb on + strand, within PP_5061 at 5771.971 kb on + strand, within PP_5061 at 5771.971 kb on + strand, within PP_5061 at 5771.971 kb on + strand, within PP_5061 at 5771.972 kb on - strand, within PP_5061 at 5771.972 kb on - strand, within PP_5061 at 5772.025 kb on + strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.026 kb on - strand, within PP_5061 at 5772.031 kb on + strand, within PP_5061 at 5772.031 kb on + strand, within PP_5061 at 5772.032 kb on - strand, within PP_5061 at 5772.032 kb on - strand, within PP_5061 at 5772.032 kb on - strand, within PP_5061 at 5772.032 kb on - strand, within PP_5061 at 5772.067 kb on + strand, within PP_5061 at 5772.068 kb on - strand, within PP_5061 at 5772.284 kb on - strand, within PP_5061 at 5772.284 kb on - strand, within PP_5061 at 5772.284 kb on - strand, within PP_5061 at 5772.300 kb on + strand, within PP_5061 at 5772.300 kb on + strand, within PP_5061 at 5772.301 kb on - strand, within PP_5061 at 5772.365 kb on + strand, within PP_5061 at 5772.419 kb on - strand, within PP_5061 at 5772.466 kb on + strand, within PP_5061 at 5772.467 kb on - strand, within PP_5061 at 5772.493 kb on + strand, within PP_5061 at 5772.494 kb on - strand, within PP_5061 at 5772.494 kb on - strand, within PP_5061 at 5772.494 kb on - strand, within PP_5061 at 5772.592 kb on + strand, within PP_5061 at 5772.592 kb on + strand, within PP_5061 at 5772.593 kb on - strand, within PP_5061 at 5772.622 kb on + strand, within PP_5061 at 5772.622 kb on + strand, within PP_5061 at 5772.636 kb on - strand at 5772.636 kb on - strand, within PP_5061 at 5772.676 kb on + strand, within PP_5061 at 5772.677 kb on - strand, within PP_5061 at 5772.725 kb on - strand, within PP_5061 at 5772.742 kb on + strand, within PP_5061 at 5772.742 kb on + strand, within PP_5061 at 5772.781 kb on + strand at 5772.781 kb on + strand at 5772.782 kb on - strand at 5772.782 kb on - strand at 5772.782 kb on - strand at 5772.782 kb on - strand at 5772.782 kb on - strand at 5772.782 kb on - strand at 5772.944 kb on + strand at 5772.944 kb on + strand at 5772.944 kb on + strand at 5772.945 kb on - strand at 5772.999 kb on - strand at 5773.049 kb on + strand at 5773.051 kb on + strand at 5773.051 kb on + strand at 5773.051 kb on + strand at 5773.051 kb on + strand at 5773.051 kb on + strand at 5773.085 kb on + strand at 5773.085 kb on + strand at 5773.086 kb on - strand at 5773.249 kb on + strand at 5773.250 kb on - strand at 5773.431 kb on + strand at 5773.432 kb on - strand at 5773.432 kb on - strand at 5773.453 kb on - strand at 5773.582 kb on + strand, within PP_5719 at 5773.624 kb on + strand, within PP_5719 at 5773.837 kb on + strand, within PP_5719 at 5773.879 kb on + strand, within PP_5719 at 5773.879 kb on + strand, within PP_5719 at 5773.880 kb on - strand, within PP_5719 at 5773.880 kb on - strand, within PP_5719
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,770,006 + PP_5060 0.82 +1.1 5,770,083 + PP_5060 0.83 -2.4 5,770,084 - PP_5060 0.83 -0.9 5,770,084 - PP_5060 0.83 +1.2 5,770,233 + PP_5060 0.86 +1.9 5,770,233 + PP_5060 0.86 +2.5 5,770,233 + PP_5060 0.86 -1.4 5,770,234 - PP_5060 0.86 -1.0 5,770,234 - PP_5060 0.86 -0.3 5,770,234 - PP_5060 0.86 -0.3 5,770,234 - PP_5060 0.86 +0.3 5,770,409 - -0.7 5,770,675 + +0.3 5,770,676 - -0.1 5,770,726 + +0.2 5,770,761 + -0.1 5,770,903 + -0.7 5,770,903 + +0.4 5,770,903 + -0.3 5,770,903 + +0.7 5,770,903 + -0.2 5,770,904 - -1.6 5,770,904 - +1.9 5,770,904 - -0.7 5,770,904 - +0.2 5,770,904 - -0.7 5,770,907 + +1.5 5,770,907 + -0.5 5,770,907 + -0.2 5,770,907 + +1.2 5,770,907 + -0.7 5,770,908 - -0.2 5,770,908 - +0.1 5,770,964 + +0.7 5,770,964 + -0.4 5,770,964 + +1.3 5,770,965 - +0.2 5,770,966 + -0.1 5,770,966 + -0.6 5,770,967 - -1.0 5,771,053 + -0.1 5,771,054 - +0.5 5,771,054 - -0.7 5,771,077 + -0.5 5,771,077 + -0.7 5,771,078 - -0.7 5,771,151 - +0.3 5,771,151 - -0.1 5,771,152 + -0.4 5,771,152 + -0.1 5,771,152 + +0.2 5,771,152 + +1.1 5,771,152 + +1.3 5,771,153 - -0.7 5,771,153 - +0.1 5,771,153 - -0.1 5,771,174 - PP_5061 0.11 +1.5 5,771,446 + PP_5061 0.24 +2.5 5,771,447 - PP_5061 0.24 -0.2 5,771,447 - PP_5061 0.24 -2.5 5,771,918 + PP_5061 0.48 -0.7 5,771,971 + PP_5061 0.50 +0.9 5,771,971 + PP_5061 0.50 -0.0 5,771,971 + PP_5061 0.50 -0.4 5,771,972 - PP_5061 0.51 +2.0 5,771,972 - PP_5061 0.51 +0.6 5,772,025 + PP_5061 0.53 -0.7 5,772,026 - PP_5061 0.53 -0.8 5,772,026 - PP_5061 0.53 -0.3 5,772,026 - PP_5061 0.53 +1.9 5,772,026 - PP_5061 0.53 +1.3 5,772,026 - PP_5061 0.53 +1.5 5,772,026 - PP_5061 0.53 +0.8 5,772,026 - PP_5061 0.53 +0.7 5,772,031 + PP_5061 0.53 +0.9 5,772,031 + PP_5061 0.53 +0.9 5,772,032 - PP_5061 0.54 +1.5 5,772,032 - PP_5061 0.54 +0.9 5,772,032 - PP_5061 0.54 +1.3 5,772,032 - PP_5061 0.54 -0.1 5,772,067 + PP_5061 0.55 -0.1 5,772,068 - PP_5061 0.55 +0.6 5,772,284 - PP_5061 0.66 +0.6 5,772,284 - PP_5061 0.66 -0.7 5,772,284 - PP_5061 0.66 +1.4 5,772,300 + PP_5061 0.67 -0.4 5,772,300 + PP_5061 0.67 -1.0 5,772,301 - PP_5061 0.67 -0.3 5,772,365 + PP_5061 0.70 +0.1 5,772,419 - PP_5061 0.73 +0.9 5,772,466 + PP_5061 0.75 +0.6 5,772,467 - PP_5061 0.75 -1.3 5,772,493 + PP_5061 0.77 +0.5 5,772,494 - PP_5061 0.77 -0.3 5,772,494 - PP_5061 0.77 +0.0 5,772,494 - PP_5061 0.77 -0.1 5,772,592 + PP_5061 0.81 -1.0 5,772,592 + PP_5061 0.81 +0.4 5,772,593 - PP_5061 0.82 +1.9 5,772,622 + PP_5061 0.83 +0.0 5,772,622 + PP_5061 0.83 -0.3 5,772,636 - -0.1 5,772,636 - PP_5061 0.84 -1.3 5,772,676 + PP_5061 0.86 +0.3 5,772,677 - PP_5061 0.86 +0.3 5,772,725 - PP_5061 0.88 +0.2 5,772,742 + PP_5061 0.89 +0.2 5,772,742 + PP_5061 0.89 +2.5 5,772,781 + -1.4 5,772,781 + -0.1 5,772,782 - -1.7 5,772,782 - -0.3 5,772,782 - -1.1 5,772,782 - -1.0 5,772,782 - -0.1 5,772,782 - -0.4 5,772,944 + -1.9 5,772,944 + -0.8 5,772,944 + +0.5 5,772,945 - -0.9 5,772,999 - -1.3 5,773,049 + -0.2 5,773,051 + -0.4 5,773,051 + -0.3 5,773,051 + -0.4 5,773,051 + +0.7 5,773,051 + +0.1 5,773,085 + -0.2 5,773,085 + -0.1 5,773,086 - -0.6 5,773,249 + +1.1 5,773,250 - +0.4 5,773,431 + +0.9 5,773,432 - -0.7 5,773,432 - -1.1 5,773,453 - -0.2 5,773,582 + PP_5719 0.22 -1.3 5,773,624 + PP_5719 0.28 +0.4 5,773,837 + PP_5719 0.61 +0.1 5,773,879 + PP_5719 0.67 -0.2 5,773,879 + PP_5719 0.67 -0.2 5,773,880 - PP_5719 0.67 -2.3 5,773,880 - PP_5719 0.67 +0.6
Or see this region's nucleotide sequence