Experiment: m.b. acetate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03320 and PGA1_c03330 are separated by 140 nucleotides PGA1_c03330 and PGA1_c03340 overlap by 11 nucleotides PGA1_c03340 and PGA1_c03350 are separated by 75 nucleotides
PGA1_c03320: PGA1_c03320 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 316,985 to 318,634
_c03320
PGA1_c03330: PGA1_c03330 - tetratricopeptide repeat-containing protein, at 318,775 to 320,523
_c03330
PGA1_c03340: PGA1_c03340 - putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 320,513 to 321,349
_c03340
PGA1_c03350: PGA1_c03350 - octaprenyl-diphosphate synthase IspB, at 321,425 to 322,423
_c03350
Position (kb) 318
319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 318.692 kb on - strand at 318.694 kb on + strand at 318.702 kb on - strand at 318.714 kb on - strand at 318.714 kb on - strand at 318.757 kb on + strand at 318.757 kb on + strand at 318.757 kb on + strand at 318.765 kb on - strand at 318.765 kb on - strand at 319.985 kb on + strand, within PGA1_c03330 at 319.987 kb on + strand, within PGA1_c03330 at 320.045 kb on + strand, within PGA1_c03330 at 320.055 kb on + strand, within PGA1_c03330 at 320.055 kb on + strand, within PGA1_c03330 at 320.107 kb on + strand, within PGA1_c03330 at 320.120 kb on - strand, within PGA1_c03330 at 320.154 kb on + strand, within PGA1_c03330 at 320.154 kb on + strand, within PGA1_c03330 at 320.282 kb on - strand, within PGA1_c03330 at 320.344 kb on + strand, within PGA1_c03330 at 320.459 kb on - strand at 320.489 kb on + strand at 320.497 kb on - strand at 321.385 kb on + strand at 321.385 kb on + strand at 321.393 kb on - strand at 321.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. acetate 100 mM remove 318,692 - +1.4 318,694 + +0.7 318,702 - +0.5 318,714 - -0.7 318,714 - -0.4 318,757 + +0.9 318,757 + +0.2 318,757 + +1.2 318,765 - -0.5 318,765 - +1.2 319,985 + PGA1_c03330 0.69 -1.0 319,987 + PGA1_c03330 0.69 -0.7 320,045 + PGA1_c03330 0.73 -0.5 320,055 + PGA1_c03330 0.73 +0.8 320,055 + PGA1_c03330 0.73 -1.4 320,107 + PGA1_c03330 0.76 -0.1 320,120 - PGA1_c03330 0.77 -0.9 320,154 + PGA1_c03330 0.79 -1.3 320,154 + PGA1_c03330 0.79 +0.3 320,282 - PGA1_c03330 0.86 -0.4 320,344 + PGA1_c03330 0.90 -2.9 320,459 - +0.2 320,489 + -0.6 320,497 - -1.1 321,385 + -0.6 321,385 + +0.0 321,393 - -0.7 321,423 + -0.9
Or see this region's nucleotide sequence