Experiment: Nitrate reduction, replicate C t3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5753 and Pf1N1B4_5754 are separated by 28 nucleotides Pf1N1B4_5754 and Pf1N1B4_5755 overlap by 7 nucleotides Pf1N1B4_5755 and Pf1N1B4_5756 are separated by 592 nucleotides
Pf1N1B4_5753 - putative membrane protein, at 193,945 to 194,382
_5753
Pf1N1B4_5754 - NnrS protein involved in response to NO, at 194,411 to 195,073
_5754
Pf1N1B4_5755 - NnrS protein involved in response to NO, at 195,067 to 195,600
_5755
Pf1N1B4_5756 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 196,193 to 196,876
_5756
Position (kb) 195
196 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.079 kb on - strand, within Pf1N1B4_5753 at 194.199 kb on - strand, within Pf1N1B4_5753 at 194.272 kb on + strand at 194.470 kb on - strand at 194.911 kb on - strand, within Pf1N1B4_5754 at 194.912 kb on + strand, within Pf1N1B4_5754 at 194.913 kb on - strand, within Pf1N1B4_5754 at 195.171 kb on + strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.172 kb on - strand, within Pf1N1B4_5755 at 195.615 kb on - strand at 195.655 kb on + strand at 195.656 kb on - strand at 195.656 kb on - strand at 195.926 kb on + strand at 195.927 kb on - strand at 196.142 kb on + strand at 196.352 kb on + strand, within Pf1N1B4_5756 at 196.353 kb on - strand, within Pf1N1B4_5756 at 196.479 kb on - strand, within Pf1N1B4_5756 at 196.538 kb on + strand, within Pf1N1B4_5756 at 196.539 kb on - strand, within Pf1N1B4_5756
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C t3 remove 194,079 - Pf1N1B4_5753 0.31 +0.1 194,079 - Pf1N1B4_5753 0.31 -2.4 194,199 - Pf1N1B4_5753 0.58 -3.2 194,272 + -3.1 194,470 - -3.3 194,911 - Pf1N1B4_5754 0.75 +0.1 194,912 + Pf1N1B4_5754 0.76 -3.3 194,913 - Pf1N1B4_5754 0.76 -3.9 195,171 + Pf1N1B4_5755 0.19 +0.1 195,172 - Pf1N1B4_5755 0.20 +0.1 195,172 - Pf1N1B4_5755 0.20 -1.9 195,172 - Pf1N1B4_5755 0.20 +0.1 195,172 - Pf1N1B4_5755 0.20 +0.1 195,615 - +0.1 195,655 + +0.1 195,656 - -2.1 195,656 - -2.0 195,926 + -2.8 195,927 - +0.1 196,142 + -4.6 196,352 + Pf1N1B4_5756 0.23 -3.5 196,353 - Pf1N1B4_5756 0.23 -4.5 196,479 - Pf1N1B4_5756 0.42 -4.2 196,538 + Pf1N1B4_5756 0.50 +0.1 196,539 - Pf1N1B4_5756 0.51 +0.1
Or see this region's nucleotide sequence