Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5089 and Pf1N1B4_5090 are separated by 68 nucleotides Pf1N1B4_5090 and Pf1N1B4_5091 are separated by 13 nucleotides
Pf1N1B4_5089 - Exopolysaccharide production protein ExoQ, at 5,640,457 to 5,641,770
_5089
Pf1N1B4_5090 - glycosyl transferase, family 2, at 5,641,839 to 5,642,741
_5090
Pf1N1B4_5091 - hypothetical protein, at 5,642,755 to 5,644,173
_5091
Position (kb) 5641
5642
5643 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5641.242 kb on - strand, within Pf1N1B4_5089 at 5641.242 kb on - strand, within Pf1N1B4_5089 at 5641.253 kb on - strand, within Pf1N1B4_5089 at 5641.253 kb on - strand, within Pf1N1B4_5089 at 5641.604 kb on - strand, within Pf1N1B4_5089 at 5641.604 kb on - strand, within Pf1N1B4_5089 at 5641.604 kb on - strand, within Pf1N1B4_5089 at 5641.612 kb on + strand, within Pf1N1B4_5089 at 5641.613 kb on - strand, within Pf1N1B4_5089 at 5641.783 kb on + strand at 5641.843 kb on - strand at 5642.058 kb on + strand at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.059 kb on - strand, within Pf1N1B4_5090 at 5642.341 kb on - strand, within Pf1N1B4_5090 at 5642.469 kb on + strand, within Pf1N1B4_5090 at 5642.775 kb on + strand at 5642.920 kb on + strand, within Pf1N1B4_5091 at 5642.920 kb on + strand, within Pf1N1B4_5091 at 5642.921 kb on - strand, within Pf1N1B4_5091 at 5643.331 kb on + strand, within Pf1N1B4_5091 at 5643.331 kb on + strand, within Pf1N1B4_5091 at 5643.331 kb on + strand, within Pf1N1B4_5091 at 5643.532 kb on + strand, within Pf1N1B4_5091 at 5643.533 kb on - strand, within Pf1N1B4_5091
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A remove 5,641,242 - Pf1N1B4_5089 0.60 -0.0 5,641,242 - Pf1N1B4_5089 0.60 -0.0 5,641,253 - Pf1N1B4_5089 0.61 -0.0 5,641,253 - Pf1N1B4_5089 0.61 -0.0 5,641,604 - Pf1N1B4_5089 0.87 -0.0 5,641,604 - Pf1N1B4_5089 0.87 -2.5 5,641,604 - Pf1N1B4_5089 0.87 -0.0 5,641,612 + Pf1N1B4_5089 0.88 -0.0 5,641,613 - Pf1N1B4_5089 0.88 -0.0 5,641,783 + -1.4 5,641,843 - -1.9 5,642,058 + -1.8 5,642,059 - Pf1N1B4_5090 0.24 -0.0 5,642,059 - Pf1N1B4_5090 0.24 -0.0 5,642,059 - Pf1N1B4_5090 0.24 -0.0 5,642,059 - Pf1N1B4_5090 0.24 -0.0 5,642,059 - Pf1N1B4_5090 0.24 -3.5 5,642,059 - Pf1N1B4_5090 0.24 -0.0 5,642,341 - Pf1N1B4_5090 0.56 -3.0 5,642,469 + Pf1N1B4_5090 0.70 -0.0 5,642,775 + -0.0 5,642,920 + Pf1N1B4_5091 0.12 -0.0 5,642,920 + Pf1N1B4_5091 0.12 -0.0 5,642,921 - Pf1N1B4_5091 0.12 -2.5 5,643,331 + Pf1N1B4_5091 0.41 -1.2 5,643,331 + Pf1N1B4_5091 0.41 -0.0 5,643,331 + Pf1N1B4_5091 0.41 -0.0 5,643,532 + Pf1N1B4_5091 0.55 -0.0 5,643,533 - Pf1N1B4_5091 0.55 -1.9
Or see this region's nucleotide sequence