Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23185 and BWI76_RS23190 overlap by 1 nucleotides BWI76_RS23190 and BWI76_RS23195 are separated by 4 nucleotides BWI76_RS23195 and BWI76_RS23200 overlap by 16 nucleotides BWI76_RS23200 and BWI76_RS23205 are separated by 91 nucleotides
BWI76_RS23185: BWI76_RS23185 - propanediol utilization: polyhedral bodies pduT, at 4,830,836 to 4,831,390
_RS23185
BWI76_RS23190: BWI76_RS23190 - propanediol utilization protein PduU, at 4,831,390 to 4,831,740
_RS23190
BWI76_RS23195: BWI76_RS23195 - ethanolamine utilization protein EutP, at 4,831,745 to 4,832,197
_RS23195
BWI76_RS23200: BWI76_RS23200 - propionate kinase, at 4,832,182 to 4,833,396
_RS23200
BWI76_RS23205: BWI76_RS23205 - propanediol utilization protein PduX, at 4,833,488 to 4,834,354
_RS23205
Position (kb) 4832
4833
4834 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4831.420 kb on + strand at 4831.420 kb on + strand at 4831.428 kb on - strand, within BWI76_RS23190 at 4831.469 kb on - strand, within BWI76_RS23190 at 4831.538 kb on + strand, within BWI76_RS23190 at 4831.538 kb on + strand, within BWI76_RS23190 at 4831.903 kb on + strand, within BWI76_RS23195 at 4831.911 kb on - strand, within BWI76_RS23195 at 4831.921 kb on - strand, within BWI76_RS23195 at 4832.055 kb on - strand, within BWI76_RS23195 at 4832.177 kb on + strand at 4832.253 kb on + strand at 4832.455 kb on + strand, within BWI76_RS23200 at 4832.711 kb on + strand, within BWI76_RS23200 at 4832.898 kb on + strand, within BWI76_RS23200 at 4832.933 kb on + strand, within BWI76_RS23200 at 4832.967 kb on + strand, within BWI76_RS23200 at 4833.292 kb on - strand at 4833.292 kb on - strand at 4833.309 kb on + strand at 4833.363 kb on + strand at 4833.580 kb on + strand, within BWI76_RS23205 at 4833.670 kb on + strand, within BWI76_RS23205 at 4833.745 kb on + strand, within BWI76_RS23205 at 4833.863 kb on - strand, within BWI76_RS23205 at 4833.887 kb on + strand, within BWI76_RS23205 at 4834.041 kb on - strand, within BWI76_RS23205 at 4834.336 kb on - strand at 4834.338 kb on + strand at 4834.340 kb on + strand at 4834.346 kb on - strand at 4834.348 kb on + strand at 4834.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,831,420 + +0.6 4,831,420 + -0.2 4,831,428 - BWI76_RS23190 0.11 +0.5 4,831,469 - BWI76_RS23190 0.23 +0.3 4,831,538 + BWI76_RS23190 0.42 +0.7 4,831,538 + BWI76_RS23190 0.42 +0.1 4,831,903 + BWI76_RS23195 0.35 -0.5 4,831,911 - BWI76_RS23195 0.37 +0.5 4,831,921 - BWI76_RS23195 0.39 -0.1 4,832,055 - BWI76_RS23195 0.68 +0.1 4,832,177 + -0.0 4,832,253 + -0.1 4,832,455 + BWI76_RS23200 0.22 +0.0 4,832,711 + BWI76_RS23200 0.44 +2.0 4,832,898 + BWI76_RS23200 0.59 +0.2 4,832,933 + BWI76_RS23200 0.62 +0.3 4,832,967 + BWI76_RS23200 0.65 +0.2 4,833,292 - +0.0 4,833,292 - +0.3 4,833,309 + -0.2 4,833,363 + -2.5 4,833,580 + BWI76_RS23205 0.11 -0.3 4,833,670 + BWI76_RS23205 0.21 +0.2 4,833,745 + BWI76_RS23205 0.30 -0.2 4,833,863 - BWI76_RS23205 0.43 +0.9 4,833,887 + BWI76_RS23205 0.46 -0.0 4,834,041 - BWI76_RS23205 0.64 +0.2 4,834,336 - -0.5 4,834,338 + +0.3 4,834,340 + -0.1 4,834,346 - +0.4 4,834,348 + -1.1 4,834,348 + +0.4
Or see this region's nucleotide sequence