Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22890 and BWI76_RS22895 are separated by 73 nucleotides BWI76_RS22895 and BWI76_RS22900 are separated by 41 nucleotides
BWI76_RS22890: BWI76_RS22890 - 5,10-methylenetetrahydromethanopterin reductase, at 4,776,743 to 4,778,149
_RS22890
BWI76_RS22895: BWI76_RS22895 - FAD-dependent oxidoreductase, at 4,778,223 to 4,779,620
_RS22895
BWI76_RS22900: BWI76_RS22900 - alkanesulfonate monooxygenase, at 4,779,662 to 4,780,834
_RS22900
Position (kb) 4778
4779
4780 Strain fitness (log2 ratio) -1
0
1
2 at 4777.243 kb on - strand, within BWI76_RS22890 at 4777.487 kb on - strand, within BWI76_RS22890 at 4777.569 kb on + strand, within BWI76_RS22890 at 4777.974 kb on + strand, within BWI76_RS22890 at 4778.050 kb on + strand at 4778.081 kb on - strand at 4778.081 kb on - strand at 4778.154 kb on - strand at 4778.154 kb on - strand at 4778.257 kb on - strand at 4778.644 kb on + strand, within BWI76_RS22895 at 4778.658 kb on - strand, within BWI76_RS22895 at 4778.715 kb on - strand, within BWI76_RS22895 at 4778.925 kb on - strand, within BWI76_RS22895 at 4778.925 kb on - strand, within BWI76_RS22895 at 4778.985 kb on - strand, within BWI76_RS22895 at 4779.074 kb on - strand, within BWI76_RS22895 at 4779.079 kb on + strand, within BWI76_RS22895 at 4779.410 kb on + strand, within BWI76_RS22895 at 4779.446 kb on + strand, within BWI76_RS22895 at 4779.934 kb on - strand, within BWI76_RS22900 at 4779.940 kb on - strand, within BWI76_RS22900 at 4780.062 kb on + strand, within BWI76_RS22900 at 4780.229 kb on + strand, within BWI76_RS22900 at 4780.237 kb on - strand, within BWI76_RS22900 at 4780.247 kb on - strand, within BWI76_RS22900 at 4780.262 kb on + strand, within BWI76_RS22900 at 4780.305 kb on + strand, within BWI76_RS22900
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 4,777,243 - BWI76_RS22890 0.36 -0.3 4,777,487 - BWI76_RS22890 0.53 -0.0 4,777,569 + BWI76_RS22890 0.59 +0.5 4,777,974 + BWI76_RS22890 0.87 -0.1 4,778,050 + -0.1 4,778,081 - -0.2 4,778,081 - -0.5 4,778,154 - +0.0 4,778,154 - +0.7 4,778,257 - +1.1 4,778,644 + BWI76_RS22895 0.30 -0.3 4,778,658 - BWI76_RS22895 0.31 +0.1 4,778,715 - BWI76_RS22895 0.35 -0.0 4,778,925 - BWI76_RS22895 0.50 +2.5 4,778,925 - BWI76_RS22895 0.50 +1.8 4,778,985 - BWI76_RS22895 0.55 +0.3 4,779,074 - BWI76_RS22895 0.61 -0.0 4,779,079 + BWI76_RS22895 0.61 -0.0 4,779,410 + BWI76_RS22895 0.85 +0.1 4,779,446 + BWI76_RS22895 0.87 -0.6 4,779,934 - BWI76_RS22900 0.23 -0.4 4,779,940 - BWI76_RS22900 0.24 +0.1 4,780,062 + BWI76_RS22900 0.34 -0.2 4,780,229 + BWI76_RS22900 0.48 +0.1 4,780,237 - BWI76_RS22900 0.49 -0.3 4,780,247 - BWI76_RS22900 0.50 +1.0 4,780,262 + BWI76_RS22900 0.51 -0.5 4,780,305 + BWI76_RS22900 0.55 +0.2
Or see this region's nucleotide sequence