Experiment: Dilute LB (1/10)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01750 and BWI76_RS01755 are separated by 348 nucleotides BWI76_RS01755 and BWI76_RS01760 overlap by 4 nucleotides BWI76_RS01760 and BWI76_RS01765 are separated by 185 nucleotides
BWI76_RS01750: BWI76_RS01750 - Cro/Cl family transcriptional regulator, at 357,467 to 358,414
_RS01750
BWI76_RS01755: BWI76_RS01755 - 23S rRNA pseudouridine synthase F, at 358,763 to 359,635
_RS01755
BWI76_RS01760: BWI76_RS01760 - hypothetical protein, at 359,632 to 359,904
_RS01760
BWI76_RS01765: BWI76_RS01765 - hypothetical protein, at 360,090 to 361,031
_RS01765
Position (kb) 358
359
360 Strain fitness (log2 ratio) -1
0
1
2 at 357.788 kb on - strand, within BWI76_RS01750 at 357.796 kb on + strand, within BWI76_RS01750 at 357.797 kb on + strand, within BWI76_RS01750 at 357.935 kb on + strand, within BWI76_RS01750 at 358.035 kb on + strand, within BWI76_RS01750 at 358.134 kb on + strand, within BWI76_RS01750 at 358.164 kb on - strand, within BWI76_RS01750 at 358.184 kb on - strand, within BWI76_RS01750 at 358.216 kb on + strand, within BWI76_RS01750 at 358.255 kb on - strand, within BWI76_RS01750 at 358.281 kb on - strand, within BWI76_RS01750 at 358.347 kb on + strand at 358.347 kb on + strand at 358.347 kb on + strand at 358.355 kb on - strand at 358.355 kb on - strand at 358.365 kb on - strand at 358.396 kb on - strand at 358.438 kb on - strand at 358.547 kb on + strand at 358.568 kb on - strand at 358.578 kb on + strand at 358.586 kb on - strand at 358.647 kb on - strand at 358.654 kb on + strand at 358.688 kb on + strand at 358.723 kb on + strand at 358.737 kb on - strand at 358.825 kb on + strand at 358.825 kb on + strand at 358.833 kb on - strand at 358.833 kb on - strand at 358.884 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.897 kb on + strand, within BWI76_RS01755 at 358.967 kb on - strand, within BWI76_RS01755 at 358.978 kb on + strand, within BWI76_RS01755 at 359.091 kb on + strand, within BWI76_RS01755 at 359.191 kb on + strand, within BWI76_RS01755 at 359.252 kb on - strand, within BWI76_RS01755 at 359.896 kb on - strand at 359.900 kb on + strand at 359.922 kb on + strand at 359.930 kb on - strand at 359.943 kb on - strand at 360.027 kb on - strand at 360.134 kb on + strand at 360.339 kb on - strand, within BWI76_RS01765 at 360.349 kb on + strand, within BWI76_RS01765 at 360.367 kb on + strand, within BWI76_RS01765 at 360.417 kb on - strand, within BWI76_RS01765 at 360.518 kb on + strand, within BWI76_RS01765 at 360.527 kb on - strand, within BWI76_RS01765 at 360.553 kb on + strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765 at 360.561 kb on - strand, within BWI76_RS01765
Per-strain Table
Position Strand Gene LocusTag Fraction Dilute LB (1/10) remove 357,788 - BWI76_RS01750 0.34 -0.1 357,796 + BWI76_RS01750 0.35 -0.1 357,797 + BWI76_RS01750 0.35 -0.1 357,935 + BWI76_RS01750 0.49 +0.0 358,035 + BWI76_RS01750 0.60 +0.5 358,134 + BWI76_RS01750 0.70 -0.3 358,164 - BWI76_RS01750 0.74 -0.1 358,184 - BWI76_RS01750 0.76 -0.4 358,216 + BWI76_RS01750 0.79 -0.3 358,255 - BWI76_RS01750 0.83 +0.1 358,281 - BWI76_RS01750 0.86 -0.9 358,347 + +0.1 358,347 + +0.2 358,347 + -0.4 358,355 - +0.3 358,355 - +0.2 358,365 - +0.8 358,396 - +0.3 358,438 - +0.1 358,547 + +0.8 358,568 - -0.1 358,578 + +0.2 358,586 - -0.4 358,647 - +0.2 358,654 + -0.0 358,688 + +1.2 358,723 + -1.7 358,737 - +0.3 358,825 + +0.1 358,825 + +1.3 358,833 - -0.0 358,833 - -0.6 358,884 + BWI76_RS01755 0.14 +1.9 358,897 + BWI76_RS01755 0.15 -0.6 358,897 + BWI76_RS01755 0.15 +0.1 358,897 + BWI76_RS01755 0.15 +0.1 358,897 + BWI76_RS01755 0.15 -0.1 358,967 - BWI76_RS01755 0.23 +0.7 358,978 + BWI76_RS01755 0.25 +0.7 359,091 + BWI76_RS01755 0.38 +0.5 359,191 + BWI76_RS01755 0.49 +0.1 359,252 - BWI76_RS01755 0.56 +0.6 359,896 - +0.1 359,900 + +0.4 359,922 + +0.4 359,930 - -0.5 359,943 - +0.1 360,027 - +0.2 360,134 + +0.5 360,339 - BWI76_RS01765 0.26 -0.1 360,349 + BWI76_RS01765 0.27 -0.0 360,367 + BWI76_RS01765 0.29 -0.4 360,417 - BWI76_RS01765 0.35 +0.1 360,518 + BWI76_RS01765 0.45 +0.1 360,527 - BWI76_RS01765 0.46 +0.0 360,553 + BWI76_RS01765 0.49 +0.2 360,561 - BWI76_RS01765 0.50 +0.6 360,561 - BWI76_RS01765 0.50 -0.0
Or see this region's nucleotide sequence