Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2537 and Dshi_2538 are separated by 46 nucleotides Dshi_2538 and Dshi_2539 are separated by 168 nucleotides Dshi_2539 and Dshi_2540 are separated by 65 nucleotides
Dshi_2537: Dshi_2537 - 4,5-dihydroxyphthalate decarboxylase (RefSeq), at 2,694,263 to 2,695,291
_2537
Dshi_2538: Dshi_2538 - fumarylacetoacetate (FAA) hydrolase (RefSeq), at 2,695,338 to 2,696,024
_2538
Dshi_2539: Dshi_2539 - GntR domain protein (RefSeq), at 2,696,193 to 2,696,882
_2539
Dshi_2540: Dshi_2540 - hypothetical protein (RefSeq), at 2,696,948 to 2,697,499
_2540
Position (kb)
2695
2696
2697 Strain fitness (log2 ratio)
-1
0
1 at 2694.432 kb on + strand, within Dshi_2537 at 2694.438 kb on + strand, within Dshi_2537 at 2694.506 kb on - strand, within Dshi_2537 at 2694.517 kb on - strand, within Dshi_2537 at 2694.532 kb on - strand, within Dshi_2537 at 2694.539 kb on + strand, within Dshi_2537 at 2694.543 kb on + strand, within Dshi_2537 at 2694.644 kb on - strand, within Dshi_2537 at 2694.687 kb on + strand, within Dshi_2537 at 2694.705 kb on - strand, within Dshi_2537 at 2694.740 kb on + strand, within Dshi_2537 at 2694.748 kb on - strand, within Dshi_2537 at 2694.758 kb on - strand, within Dshi_2537 at 2694.758 kb on - strand, within Dshi_2537 at 2694.867 kb on + strand, within Dshi_2537 at 2694.869 kb on - strand, within Dshi_2537 at 2694.984 kb on - strand, within Dshi_2537 at 2695.053 kb on - strand, within Dshi_2537 at 2695.064 kb on - strand, within Dshi_2537 at 2695.204 kb on - strand at 2695.240 kb on + strand at 2695.249 kb on - strand at 2695.250 kb on - strand at 2695.409 kb on + strand, within Dshi_2538 at 2695.434 kb on + strand, within Dshi_2538 at 2695.603 kb on + strand, within Dshi_2538 at 2695.657 kb on + strand, within Dshi_2538 at 2695.665 kb on - strand, within Dshi_2538 at 2695.665 kb on - strand, within Dshi_2538 at 2695.675 kb on - strand, within Dshi_2538 at 2695.679 kb on - strand, within Dshi_2538 at 2695.799 kb on + strand, within Dshi_2538 at 2695.838 kb on + strand, within Dshi_2538 at 2695.896 kb on - strand, within Dshi_2538 at 2696.203 kb on + strand at 2696.206 kb on - strand at 2696.286 kb on - strand, within Dshi_2539 at 2696.355 kb on + strand, within Dshi_2539 at 2696.412 kb on + strand, within Dshi_2539 at 2696.412 kb on + strand, within Dshi_2539 at 2696.420 kb on - strand, within Dshi_2539 at 2696.420 kb on - strand, within Dshi_2539 at 2696.420 kb on - strand, within Dshi_2539 at 2696.439 kb on - strand, within Dshi_2539 at 2696.477 kb on + strand, within Dshi_2539 at 2696.485 kb on - strand, within Dshi_2539 at 2696.521 kb on + strand, within Dshi_2539 at 2696.547 kb on + strand, within Dshi_2539 at 2696.605 kb on + strand, within Dshi_2539 at 2696.613 kb on - strand, within Dshi_2539 at 2696.655 kb on + strand, within Dshi_2539 at 2696.686 kb on + strand, within Dshi_2539 at 2696.712 kb on + strand, within Dshi_2539 at 2696.720 kb on - strand, within Dshi_2539 at 2696.733 kb on - strand, within Dshi_2539 at 2696.757 kb on + strand, within Dshi_2539 at 2696.772 kb on - strand, within Dshi_2539 at 2696.772 kb on - strand, within Dshi_2539 at 2696.809 kb on + strand, within Dshi_2539 at 2696.843 kb on + strand at 2696.884 kb on - strand at 2696.889 kb on - strand at 2696.905 kb on - strand at 2696.924 kb on + strand at 2697.012 kb on + strand, within Dshi_2540
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 2,694,432 + Dshi_2537 0.16 -0.5 2,694,438 + Dshi_2537 0.17 +0.7 2,694,506 - Dshi_2537 0.24 +0.5 2,694,517 - Dshi_2537 0.25 -0.6 2,694,532 - Dshi_2537 0.26 -0.0 2,694,539 + Dshi_2537 0.27 +0.2 2,694,543 + Dshi_2537 0.27 +0.9 2,694,644 - Dshi_2537 0.37 +0.0 2,694,687 + Dshi_2537 0.41 +0.5 2,694,705 - Dshi_2537 0.43 +0.1 2,694,740 + Dshi_2537 0.46 -0.0 2,694,748 - Dshi_2537 0.47 +0.6 2,694,758 - Dshi_2537 0.48 +1.0 2,694,758 - Dshi_2537 0.48 -0.3 2,694,867 + Dshi_2537 0.59 +0.1 2,694,869 - Dshi_2537 0.59 +0.1 2,694,984 - Dshi_2537 0.70 +0.6 2,695,053 - Dshi_2537 0.77 +0.2 2,695,064 - Dshi_2537 0.78 +1.0 2,695,204 - -0.1 2,695,240 + +0.0 2,695,249 - +0.7 2,695,250 - +0.6 2,695,409 + Dshi_2538 0.10 -0.2 2,695,434 + Dshi_2538 0.14 -0.2 2,695,603 + Dshi_2538 0.39 -0.6 2,695,657 + Dshi_2538 0.46 -0.1 2,695,665 - Dshi_2538 0.48 +0.2 2,695,665 - Dshi_2538 0.48 -0.0 2,695,675 - Dshi_2538 0.49 -1.4 2,695,679 - Dshi_2538 0.50 -0.0 2,695,799 + Dshi_2538 0.67 -1.3 2,695,838 + Dshi_2538 0.73 -0.0 2,695,896 - Dshi_2538 0.81 -0.3 2,696,203 + +0.5 2,696,206 - +0.2 2,696,286 - Dshi_2539 0.13 +0.2 2,696,355 + Dshi_2539 0.23 +0.5 2,696,412 + Dshi_2539 0.32 +0.6 2,696,412 + Dshi_2539 0.32 +0.2 2,696,420 - Dshi_2539 0.33 -0.1 2,696,420 - Dshi_2539 0.33 -0.5 2,696,420 - Dshi_2539 0.33 +0.0 2,696,439 - Dshi_2539 0.36 +0.1 2,696,477 + Dshi_2539 0.41 -0.2 2,696,485 - Dshi_2539 0.42 +0.2 2,696,521 + Dshi_2539 0.48 -0.3 2,696,547 + Dshi_2539 0.51 +0.2 2,696,605 + Dshi_2539 0.60 +0.2 2,696,613 - Dshi_2539 0.61 -0.1 2,696,655 + Dshi_2539 0.67 -0.1 2,696,686 + Dshi_2539 0.71 -0.4 2,696,712 + Dshi_2539 0.75 +0.0 2,696,720 - Dshi_2539 0.76 +1.0 2,696,733 - Dshi_2539 0.78 +0.5 2,696,757 + Dshi_2539 0.82 -0.0 2,696,772 - Dshi_2539 0.84 +0.2 2,696,772 - Dshi_2539 0.84 -0.1 2,696,809 + Dshi_2539 0.89 -0.2 2,696,843 + +0.5 2,696,884 - -0.2 2,696,889 - -0.2 2,696,905 - +0.2 2,696,924 + +0.9 2,697,012 + Dshi_2540 0.12 +1.6
Or see this region's nucleotide sequence