Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2425 and Dshi_2426 are separated by 80 nucleotides Dshi_2426 and Dshi_2427 are separated by 185 nucleotides Dshi_2427 and Dshi_2428 are separated by 5 nucleotides
Dshi_2425: Dshi_2425 - Hemolysin-type calcium-binding region (RefSeq), at 2,565,692 to 2,567,371
_2425
Dshi_2426: Dshi_2426 - peptidase S8 and S53 subtilisin kexin sedolisin (RefSeq), at 2,567,452 to 2,568,609
_2426
Dshi_2427: Dshi_2427 - hypothetical protein (RefSeq), at 2,568,795 to 2,569,361
_2427
Dshi_2428: Dshi_2428 - RNA polymerase, sigma-24 subunit, ECF subfamily (RefSeq), at 2,569,367 to 2,569,903
_2428
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-2
-1
0
1 at 2566.466 kb on + strand, within Dshi_2425 at 2566.489 kb on - strand, within Dshi_2425 at 2566.489 kb on - strand, within Dshi_2425 at 2566.489 kb on - strand, within Dshi_2425 at 2566.635 kb on + strand, within Dshi_2425 at 2566.658 kb on - strand, within Dshi_2425 at 2566.669 kb on + strand, within Dshi_2425 at 2566.759 kb on - strand, within Dshi_2425 at 2566.795 kb on + strand at 2566.803 kb on - strand, within Dshi_2425 at 2566.817 kb on + strand, within Dshi_2425 at 2566.825 kb on - strand, within Dshi_2425 at 2566.872 kb on - strand, within Dshi_2425 at 2567.011 kb on - strand, within Dshi_2425 at 2567.086 kb on - strand, within Dshi_2425 at 2567.162 kb on + strand, within Dshi_2425 at 2567.229 kb on + strand at 2567.248 kb on - strand at 2567.258 kb on - strand at 2567.280 kb on - strand at 2567.302 kb on + strand at 2567.315 kb on + strand at 2567.400 kb on - strand at 2567.625 kb on + strand, within Dshi_2426 at 2567.711 kb on - strand, within Dshi_2426 at 2567.786 kb on + strand, within Dshi_2426 at 2567.899 kb on - strand, within Dshi_2426 at 2568.006 kb on + strand, within Dshi_2426 at 2568.014 kb on - strand, within Dshi_2426 at 2568.123 kb on + strand, within Dshi_2426 at 2568.136 kb on - strand, within Dshi_2426 at 2568.207 kb on - strand, within Dshi_2426 at 2568.224 kb on + strand, within Dshi_2426 at 2568.277 kb on + strand, within Dshi_2426 at 2568.390 kb on + strand, within Dshi_2426 at 2568.507 kb on - strand at 2568.542 kb on - strand at 2568.552 kb on - strand at 2568.552 kb on - strand at 2568.669 kb on + strand at 2568.669 kb on + strand at 2568.669 kb on + strand at 2568.677 kb on - strand at 2568.692 kb on + strand at 2568.692 kb on + strand at 2568.692 kb on + strand at 2568.692 kb on - strand at 2568.700 kb on - strand at 2568.726 kb on + strand at 2568.726 kb on + strand at 2568.759 kb on + strand at 2568.881 kb on + strand, within Dshi_2427 at 2568.968 kb on + strand, within Dshi_2427 at 2568.981 kb on - strand, within Dshi_2427 at 2568.987 kb on + strand, within Dshi_2427 at 2569.081 kb on - strand, within Dshi_2427 at 2569.249 kb on + strand, within Dshi_2427 at 2569.280 kb on - strand, within Dshi_2427 at 2569.394 kb on + strand at 2569.520 kb on - strand, within Dshi_2428 at 2569.522 kb on + strand, within Dshi_2428
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 2,566,466 + Dshi_2425 0.46 +0.2 2,566,489 - Dshi_2425 0.47 -0.8 2,566,489 - Dshi_2425 0.47 +0.8 2,566,489 - Dshi_2425 0.47 -0.8 2,566,635 + Dshi_2425 0.56 +0.1 2,566,658 - Dshi_2425 0.57 +0.2 2,566,669 + Dshi_2425 0.58 -0.7 2,566,759 - Dshi_2425 0.64 -0.2 2,566,795 + +0.3 2,566,803 - Dshi_2425 0.66 -2.3 2,566,817 + Dshi_2425 0.67 +0.1 2,566,825 - Dshi_2425 0.67 +0.1 2,566,872 - Dshi_2425 0.70 +0.3 2,567,011 - Dshi_2425 0.79 +0.1 2,567,086 - Dshi_2425 0.83 -2.2 2,567,162 + Dshi_2425 0.88 +0.2 2,567,229 + +0.0 2,567,248 - -0.0 2,567,258 - +0.4 2,567,280 - -0.0 2,567,302 + +0.5 2,567,315 + +0.1 2,567,400 - -0.1 2,567,625 + Dshi_2426 0.15 +1.0 2,567,711 - Dshi_2426 0.22 -0.7 2,567,786 + Dshi_2426 0.29 +0.4 2,567,899 - Dshi_2426 0.39 -1.1 2,568,006 + Dshi_2426 0.48 -0.4 2,568,014 - Dshi_2426 0.49 +0.3 2,568,123 + Dshi_2426 0.58 -0.6 2,568,136 - Dshi_2426 0.59 -0.3 2,568,207 - Dshi_2426 0.65 -1.3 2,568,224 + Dshi_2426 0.67 -0.4 2,568,277 + Dshi_2426 0.71 -1.3 2,568,390 + Dshi_2426 0.81 +0.2 2,568,507 - -0.6 2,568,542 - +0.6 2,568,552 - +0.5 2,568,552 - +0.3 2,568,669 + +0.3 2,568,669 + -0.2 2,568,669 + +0.5 2,568,677 - -0.4 2,568,692 + +0.4 2,568,692 + +0.4 2,568,692 + +0.4 2,568,692 - +0.6 2,568,700 - +0.2 2,568,726 + -0.2 2,568,726 + -2.4 2,568,759 + -0.4 2,568,881 + Dshi_2427 0.15 +0.8 2,568,968 + Dshi_2427 0.31 +0.1 2,568,981 - Dshi_2427 0.33 +0.3 2,568,987 + Dshi_2427 0.34 +0.2 2,569,081 - Dshi_2427 0.50 -0.6 2,569,249 + Dshi_2427 0.80 +0.2 2,569,280 - Dshi_2427 0.86 +0.3 2,569,394 + -0.2 2,569,520 - Dshi_2428 0.28 +0.5 2,569,522 + Dshi_2428 0.29 -0.8
Or see this region's nucleotide sequence