Experiment: Dino_ML3_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1556 and Dshi_1557 overlap by 14 nucleotides Dshi_1557 and Dshi_1558 overlap by 34 nucleotides Dshi_1558 and Dshi_1559 are separated by 115 nucleotides
Dshi_1556: Dshi_1556 - AMP-dependent synthetase and ligase (RefSeq), at 1,617,876 to 1,619,435
_1556
Dshi_1557: Dshi_1557 - DSBA oxidoreductase (RefSeq), at 1,619,422 to 1,620,090
_1557
Dshi_1558: Dshi_1558 - hypothetical protein (RefSeq), at 1,620,057 to 1,620,377
_1558
Dshi_1559: Dshi_1559 - drug resistance transporter, Bcr/CflA subfamily (RefSeq), at 1,620,493 to 1,621,701
_1559
Position (kb)
1619
1620
1621 Strain fitness (log2 ratio)
-1
0
1 at 1618.428 kb on - strand, within Dshi_1556 at 1618.430 kb on + strand, within Dshi_1556 at 1618.713 kb on + strand, within Dshi_1556 at 1618.878 kb on + strand, within Dshi_1556 at 1618.886 kb on - strand, within Dshi_1556 at 1618.939 kb on + strand, within Dshi_1556 at 1619.104 kb on + strand, within Dshi_1556 at 1619.104 kb on + strand, within Dshi_1556 at 1619.233 kb on + strand, within Dshi_1556 at 1619.692 kb on + strand, within Dshi_1557 at 1619.752 kb on - strand, within Dshi_1557 at 1620.354 kb on + strand at 1620.374 kb on + strand at 1620.505 kb on + strand at 1620.651 kb on - strand, within Dshi_1559 at 1620.768 kb on + strand, within Dshi_1559 at 1620.843 kb on + strand, within Dshi_1559 at 1620.878 kb on - strand, within Dshi_1559 at 1620.878 kb on - strand, within Dshi_1559
Per-strain Table
Position Strand Gene LocusTag Fraction Dino_ML3_MIT diversity barseq remove 1,618,428 - Dshi_1556 0.35 +0.8 1,618,430 + Dshi_1556 0.36 -0.1 1,618,713 + Dshi_1556 0.54 +0.3 1,618,878 + Dshi_1556 0.64 +0.2 1,618,886 - Dshi_1556 0.65 -0.2 1,618,939 + Dshi_1556 0.68 +0.2 1,619,104 + Dshi_1556 0.79 +0.7 1,619,104 + Dshi_1556 0.79 +0.1 1,619,233 + Dshi_1556 0.87 -0.0 1,619,692 + Dshi_1557 0.40 -0.6 1,619,752 - Dshi_1557 0.49 -1.2 1,620,354 + +0.6 1,620,374 + -0.0 1,620,505 + -0.4 1,620,651 - Dshi_1559 0.13 -0.9 1,620,768 + Dshi_1559 0.23 +0.4 1,620,843 + Dshi_1559 0.29 +0.2 1,620,878 - Dshi_1559 0.32 +0.2 1,620,878 - Dshi_1559 0.32 -0.1
Or see this region's nucleotide sequence