Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5495 and Pf1N1B4_5496 are separated by 68 nucleotides
Pf1N1B4_5495 - Cyclic AMP receptor protein, at 243,236 to 243,814
_5495
Pf1N1B4_5496 - hypothetical protein, at 243,883 to 244,107
_5496
Position (kb) 243
244 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 242.278 kb on + strand at 242.329 kb on + strand at 242.329 kb on + strand at 242.506 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.520 kb on + strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.521 kb on - strand at 242.622 kb on - strand at 242.790 kb on + strand at 242.790 kb on + strand at 242.791 kb on - strand at 242.817 kb on + strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.818 kb on - strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.831 kb on + strand at 242.832 kb on - strand at 242.870 kb on + strand at 242.871 kb on - strand at 242.871 kb on - strand at 243.050 kb on - strand at 243.064 kb on + strand at 243.065 kb on - strand at 243.065 kb on - strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.081 kb on + strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.082 kb on - strand at 243.155 kb on - strand at 243.292 kb on + strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.296 kb on + strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.297 kb on - strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.351 kb on + strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.352 kb on - strand, within Pf1N1B4_5495 at 243.360 kb on + strand, within Pf1N1B4_5495 at 243.361 kb on - strand, within Pf1N1B4_5495 at 243.369 kb on + strand at 243.370 kb on - strand, within Pf1N1B4_5495 at 243.475 kb on - strand, within Pf1N1B4_5495 at 243.583 kb on + strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.584 kb on - strand, within Pf1N1B4_5495 at 243.719 kb on - strand, within Pf1N1B4_5495 at 243.790 kb on + strand at 243.791 kb on - strand at 243.791 kb on - strand at 243.834 kb on + strand at 243.834 kb on + strand at 243.835 kb on - strand at 243.835 kb on - strand at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.922 kb on + strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 243.923 kb on - strand, within Pf1N1B4_5496 at 244.000 kb on + strand, within Pf1N1B4_5496 at 244.001 kb on - strand, within Pf1N1B4_5496 at 244.106 kb on - strand at 244.129 kb on - strand at 244.129 kb on - strand at 244.154 kb on + strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.155 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.183 kb on - strand at 244.300 kb on + strand at 244.431 kb on - strand at 244.431 kb on - strand at 244.445 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand at 244.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 242,278 + -2.0 242,329 + -3.6 242,329 + -0.1 242,506 + -0.1 242,520 + -0.1 242,520 + -0.1 242,520 + -0.1 242,521 - -0.1 242,521 - -2.0 242,521 - -0.1 242,521 - -2.7 242,521 - -0.1 242,521 - -0.1 242,521 - -0.1 242,622 - -0.1 242,790 + -0.6 242,790 + -2.0 242,791 - -1.3 242,817 + -1.0 242,818 - -1.3 242,818 - -1.9 242,818 - -2.4 242,818 - -0.1 242,831 + -0.1 242,831 + -0.8 242,831 + -0.1 242,832 - -1.1 242,870 + -0.7 242,871 - -0.1 242,871 - -0.1 243,050 - -0.1 243,064 + -1.0 243,065 - -1.7 243,065 - -0.9 243,081 + -1.6 243,081 + -0.1 243,081 + -1.7 243,082 - -0.1 243,082 - -0.1 243,082 - -1.9 243,082 - -3.4 243,082 - -2.9 243,082 - -0.1 243,155 - -0.1 243,292 + -0.1 243,293 - -0.1 243,293 - -0.1 243,293 - -0.1 243,296 + Pf1N1B4_5495 0.10 -0.1 243,296 + Pf1N1B4_5495 0.10 -0.1 243,296 + Pf1N1B4_5495 0.10 -0.1 243,296 + Pf1N1B4_5495 0.10 -0.1 243,296 + Pf1N1B4_5495 0.10 -0.1 243,297 - Pf1N1B4_5495 0.11 -0.1 243,297 - Pf1N1B4_5495 0.11 -3.1 243,297 - Pf1N1B4_5495 0.11 -0.1 243,351 + Pf1N1B4_5495 0.20 -1.9 243,351 + Pf1N1B4_5495 0.20 -2.0 243,351 + Pf1N1B4_5495 0.20 -3.7 243,351 + Pf1N1B4_5495 0.20 -0.1 243,352 - Pf1N1B4_5495 0.20 -0.1 243,352 - Pf1N1B4_5495 0.20 -2.9 243,360 + Pf1N1B4_5495 0.21 -2.1 243,361 - Pf1N1B4_5495 0.22 -2.1 243,369 + -0.1 243,370 - Pf1N1B4_5495 0.23 -0.1 243,475 - Pf1N1B4_5495 0.41 -0.1 243,583 + Pf1N1B4_5495 0.60 -0.1 243,584 - Pf1N1B4_5495 0.60 -2.4 243,584 - Pf1N1B4_5495 0.60 -2.7 243,719 - Pf1N1B4_5495 0.83 -2.4 243,790 + -0.5 243,791 - -0.1 243,791 - -3.1 243,834 + -2.0 243,834 + -1.1 243,835 - -0.1 243,835 - -0.1 243,922 + Pf1N1B4_5496 0.17 -1.4 243,922 + Pf1N1B4_5496 0.17 -2.0 243,922 + Pf1N1B4_5496 0.17 -1.0 243,923 - Pf1N1B4_5496 0.18 -0.1 243,923 - Pf1N1B4_5496 0.18 -0.1 243,923 - Pf1N1B4_5496 0.18 -3.6 244,000 + Pf1N1B4_5496 0.52 -0.1 244,001 - Pf1N1B4_5496 0.52 -0.1 244,106 - -0.1 244,129 - -2.6 244,129 - +0.9 244,154 + -0.6 244,155 - -0.1 244,155 - -0.1 244,155 - -0.1 244,183 - -2.2 244,183 - -0.1 244,183 - -1.6 244,183 - -0.1 244,183 - -2.2 244,183 - -0.1 244,300 + -0.1 244,431 - -2.5 244,431 - -0.1 244,445 - -0.1 244,571 - -0.1 244,571 - -0.1 244,571 - -0.1 244,571 - -2.3
Or see this region's nucleotide sequence