Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5136 and Pf1N1B4_5137 are separated by 27 nucleotides Pf1N1B4_5137 and Pf1N1B4_5138 are separated by 237 nucleotides
Pf1N1B4_5136 - Carboxynorspermidine dehydrogenase, at 5,714,810 to 5,716,045
_5136
Pf1N1B4_5137 - Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-), at 5,716,073 to 5,717,170
_5137
Pf1N1B4_5138 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,717,408 to 5,718,499
_5138
Position (kb) 5716
5717
5718 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5715.234 kb on - strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.252 kb on + strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.253 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.340 kb on - strand, within Pf1N1B4_5136 at 5715.487 kb on - strand, within Pf1N1B4_5136 at 5715.536 kb on - strand, within Pf1N1B4_5136 at 5715.546 kb on + strand, within Pf1N1B4_5136 at 5715.657 kb on + strand at 5715.866 kb on + strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.867 kb on - strand, within Pf1N1B4_5136 at 5715.956 kb on - strand at 5716.028 kb on - strand at 5716.074 kb on + strand at 5716.093 kb on - strand at 5716.093 kb on - strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.308 kb on + strand, within Pf1N1B4_5137 at 5716.309 kb on - strand, within Pf1N1B4_5137 at 5716.419 kb on + strand, within Pf1N1B4_5137 at 5716.851 kb on + strand at 5716.942 kb on - strand, within Pf1N1B4_5137 at 5717.005 kb on + strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.006 kb on - strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.019 kb on + strand, within Pf1N1B4_5137 at 5717.020 kb on - strand at 5717.020 kb on - strand at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.020 kb on - strand, within Pf1N1B4_5137 at 5717.151 kb on + strand at 5717.151 kb on + strand at 5717.151 kb on + strand at 5717.152 kb on - strand at 5717.152 kb on - strand at 5717.154 kb on + strand at 5717.155 kb on - strand at 5717.338 kb on + strand at 5717.339 kb on - strand at 5717.633 kb on + strand, within Pf1N1B4_5138 at 5717.694 kb on + strand, within Pf1N1B4_5138 at 5717.721 kb on + strand, within Pf1N1B4_5138 at 5717.721 kb on + strand, within Pf1N1B4_5138 at 5717.767 kb on - strand at 5717.911 kb on - strand, within Pf1N1B4_5138
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 5,715,234 - Pf1N1B4_5136 0.34 +0.0 5,715,252 + Pf1N1B4_5136 0.36 -3.6 5,715,252 + Pf1N1B4_5136 0.36 +1.0 5,715,252 + Pf1N1B4_5136 0.36 +0.0 5,715,253 - Pf1N1B4_5136 0.36 -3.5 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,253 - -4.1 5,715,253 - Pf1N1B4_5136 0.36 -2.2 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,253 - Pf1N1B4_5136 0.36 +0.0 5,715,340 - Pf1N1B4_5136 0.43 +0.0 5,715,340 - Pf1N1B4_5136 0.43 +0.0 5,715,487 - Pf1N1B4_5136 0.55 -1.0 5,715,536 - Pf1N1B4_5136 0.59 -1.3 5,715,546 + Pf1N1B4_5136 0.60 +0.0 5,715,657 + +0.0 5,715,866 + Pf1N1B4_5136 0.85 -3.6 5,715,867 - Pf1N1B4_5136 0.86 +0.0 5,715,867 - Pf1N1B4_5136 0.86 -2.8 5,715,867 - Pf1N1B4_5136 0.86 -3.7 5,715,956 - +0.0 5,716,028 - -2.4 5,716,074 + -2.9 5,716,093 - +0.0 5,716,093 - -2.6 5,716,308 + Pf1N1B4_5137 0.21 -3.0 5,716,308 + -2.7 5,716,308 + Pf1N1B4_5137 0.21 -2.1 5,716,308 + +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,308 + Pf1N1B4_5137 0.21 +0.0 5,716,309 - Pf1N1B4_5137 0.21 -2.4 5,716,419 + Pf1N1B4_5137 0.32 -4.2 5,716,851 + +0.0 5,716,942 - Pf1N1B4_5137 0.79 +0.0 5,717,005 + Pf1N1B4_5137 0.85 -2.5 5,717,006 - Pf1N1B4_5137 0.85 +0.0 5,717,006 - Pf1N1B4_5137 0.85 -3.6 5,717,006 - Pf1N1B4_5137 0.85 -3.6 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -0.9 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -3.5 5,717,019 + Pf1N1B4_5137 0.86 +0.0 5,717,019 + Pf1N1B4_5137 0.86 -2.3 5,717,019 + Pf1N1B4_5137 0.86 -1.8 5,717,020 - -2.2 5,717,020 - -2.3 5,717,020 - Pf1N1B4_5137 0.86 -3.4 5,717,020 - Pf1N1B4_5137 0.86 -2.3 5,717,020 - Pf1N1B4_5137 0.86 +0.0 5,717,151 + +0.0 5,717,151 + +0.0 5,717,151 + +0.0 5,717,152 - +0.0 5,717,152 - -2.9 5,717,154 + +0.0 5,717,155 - +0.0 5,717,338 + +0.2 5,717,339 - +0.0 5,717,633 + Pf1N1B4_5138 0.21 +0.0 5,717,694 + Pf1N1B4_5138 0.26 -1.7 5,717,721 + Pf1N1B4_5138 0.29 -2.9 5,717,721 + Pf1N1B4_5138 0.29 -1.3 5,717,767 - +0.0 5,717,911 - Pf1N1B4_5138 0.46 +0.0
Or see this region's nucleotide sequence