Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_283 and Pf1N1B4_284 are separated by 113 nucleotides Pf1N1B4_284 and Pf1N1B4_285 are separated by 96 nucleotides
Pf1N1B4_283 - Cys regulon transcriptional activator CysB, at 339,949 to 340,923
_283
Pf1N1B4_284 - Universal stress protein family, at 341,037 to 341,540
_284
Pf1N1B4_285 - 5'-nucleotidase (EC 3.1.3.5), at 341,637 to 342,542
_285
Position (kb) 341
342 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 340.944 kb on - strand at 340.944 kb on - strand at 340.944 kb on - strand at 340.969 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.971 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.977 kb on - strand at 340.979 kb on - strand at 341.003 kb on - strand at 341.039 kb on - strand at 341.039 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.081 kb on - strand at 341.167 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.337 kb on - strand, within Pf1N1B4_284 at 341.383 kb on + strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.384 kb on - strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.440 kb on + strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.441 kb on - strand, within Pf1N1B4_284 at 341.500 kb on + strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.501 kb on - strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.521 kb on + strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.522 kb on - strand at 341.583 kb on + strand at 341.584 kb on - strand at 341.584 kb on - strand at 341.639 kb on - strand at 341.654 kb on + strand at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.733 kb on + strand, within Pf1N1B4_285 at 341.734 kb on - strand, within Pf1N1B4_285 at 342.019 kb on + strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.020 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.117 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.206 kb on - strand, within Pf1N1B4_285 at 342.260 kb on - strand, within Pf1N1B4_285 at 342.260 kb on - strand, within Pf1N1B4_285 at 342.269 kb on + strand at 342.269 kb on + strand, within Pf1N1B4_285 at 342.269 kb on + strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.270 kb on - strand, within Pf1N1B4_285 at 342.321 kb on + strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.322 kb on - strand, within Pf1N1B4_285 at 342.428 kb on - strand, within Pf1N1B4_285 at 342.428 kb on - strand, within Pf1N1B4_285 at 342.461 kb on - strand at 342.461 kb on - strand at 342.461 kb on - strand at 342.461 kb on - strand at 342.528 kb on + strand at 342.529 kb on - strand at 342.529 kb on - strand at 342.529 kb on - strand at 342.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 340,944 - -2.1 340,944 - -1.3 340,944 - -0.1 340,969 - -0.1 340,971 - -2.8 340,971 - -1.5 340,971 - -0.6 340,971 - -0.9 340,971 - -1.8 340,971 - -1.1 340,971 - -2.8 340,977 - -0.1 340,977 - -1.4 340,977 - -1.2 340,979 - -0.1 341,003 - -1.6 341,039 - -1.3 341,039 - -1.7 341,081 - -3.1 341,081 - -0.1 341,081 - -1.8 341,081 - -0.1 341,167 - Pf1N1B4_284 0.26 -2.8 341,337 - Pf1N1B4_284 0.60 -2.4 341,337 - Pf1N1B4_284 0.60 -2.2 341,383 + Pf1N1B4_284 0.69 -0.1 341,384 - Pf1N1B4_284 0.69 -2.9 341,384 - Pf1N1B4_284 0.69 -2.6 341,384 - Pf1N1B4_284 0.69 -3.0 341,384 - Pf1N1B4_284 0.69 -1.4 341,384 - Pf1N1B4_284 0.69 -0.1 341,384 - Pf1N1B4_284 0.69 -1.6 341,384 - Pf1N1B4_284 0.69 -2.4 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -2.9 341,440 + -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -2.7 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + Pf1N1B4_284 0.80 -5.0 341,440 + Pf1N1B4_284 0.80 -0.1 341,440 + -0.1 341,440 + Pf1N1B4_284 0.80 -3.3 341,440 + Pf1N1B4_284 0.80 -2.6 341,440 + Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -1.1 341,441 - Pf1N1B4_284 0.80 -2.4 341,441 - Pf1N1B4_284 0.80 -4.6 341,441 - Pf1N1B4_284 0.80 -2.1 341,441 - Pf1N1B4_284 0.80 -4.0 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -2.7 341,441 - Pf1N1B4_284 0.80 -2.2 341,441 - Pf1N1B4_284 0.80 -1.5 341,441 - Pf1N1B4_284 0.80 -2.9 341,441 - Pf1N1B4_284 0.80 -5.6 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -2.4 341,441 - Pf1N1B4_284 0.80 -2.8 341,441 - Pf1N1B4_284 0.80 -2.2 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -4.0 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -3.3 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - -0.1 341,441 - Pf1N1B4_284 0.80 -3.7 341,441 - Pf1N1B4_284 0.80 -4.0 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -3.0 341,441 - Pf1N1B4_284 0.80 -1.8 341,441 - Pf1N1B4_284 0.80 -2.3 341,441 - Pf1N1B4_284 0.80 -2.6 341,441 - -0.1 341,441 - Pf1N1B4_284 0.80 -2.4 341,441 - Pf1N1B4_284 0.80 -3.5 341,441 - Pf1N1B4_284 0.80 -3.2 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -0.1 341,441 - Pf1N1B4_284 0.80 -1.4 341,441 - Pf1N1B4_284 0.80 -3.0 341,441 - Pf1N1B4_284 0.80 -2.6 341,441 - Pf1N1B4_284 0.80 -3.9 341,441 - Pf1N1B4_284 0.80 -4.6 341,441 - Pf1N1B4_284 0.80 -3.5 341,441 - Pf1N1B4_284 0.80 -3.2 341,441 - Pf1N1B4_284 0.80 -4.0 341,500 + -0.1 341,501 - -2.8 341,501 - -4.3 341,501 - -1.8 341,501 - -0.1 341,501 - -0.1 341,501 - -0.1 341,501 - -2.9 341,501 - -3.4 341,501 - -2.6 341,521 + -0.1 341,521 + -0.1 341,521 + -4.4 341,521 + -4.4 341,521 + -0.1 341,521 + -0.1 341,521 + -2.7 341,521 + -0.1 341,522 - -2.9 341,522 - -3.9 341,522 - -3.8 341,522 - -0.5 341,522 - -3.3 341,522 - -2.3 341,522 - -0.1 341,522 - -2.4 341,522 - -2.0 341,522 - -1.7 341,522 - -2.6 341,522 - -3.6 341,522 - -3.3 341,522 - -2.1 341,522 - -0.1 341,522 - -1.1 341,522 - -3.5 341,522 - -0.1 341,522 - -3.2 341,522 - -2.0 341,522 - -0.1 341,522 - -1.1 341,583 + -5.7 341,584 - -0.2 341,584 - -0.1 341,639 - -0.1 341,654 + -1.1 341,733 + Pf1N1B4_285 0.11 -0.1 341,733 + Pf1N1B4_285 0.11 -0.1 341,733 + Pf1N1B4_285 0.11 -2.0 341,734 - Pf1N1B4_285 0.11 -1.3 342,019 + Pf1N1B4_285 0.42 -1.5 342,020 - Pf1N1B4_285 0.42 -0.4 342,020 - Pf1N1B4_285 0.42 -0.7 342,020 - Pf1N1B4_285 0.42 -0.1 342,117 - Pf1N1B4_285 0.53 -0.6 342,117 - Pf1N1B4_285 0.53 -1.9 342,117 - Pf1N1B4_285 0.53 -1.2 342,117 - Pf1N1B4_285 0.53 -0.1 342,117 - Pf1N1B4_285 0.53 -0.1 342,206 - Pf1N1B4_285 0.63 -0.1 342,206 - Pf1N1B4_285 0.63 -0.1 342,206 - Pf1N1B4_285 0.63 -0.9 342,260 - Pf1N1B4_285 0.69 -0.1 342,260 - Pf1N1B4_285 0.69 -1.9 342,269 + -2.2 342,269 + Pf1N1B4_285 0.70 -0.1 342,269 + Pf1N1B4_285 0.70 -1.4 342,270 - Pf1N1B4_285 0.70 -2.0 342,270 - Pf1N1B4_285 0.70 -0.1 342,270 - Pf1N1B4_285 0.70 -0.1 342,270 - Pf1N1B4_285 0.70 -0.1 342,270 - Pf1N1B4_285 0.70 -0.1 342,270 - Pf1N1B4_285 0.70 -1.0 342,270 - Pf1N1B4_285 0.70 -0.1 342,321 + Pf1N1B4_285 0.75 -2.0 342,322 - Pf1N1B4_285 0.76 -1.3 342,322 - Pf1N1B4_285 0.76 -2.0 342,322 - Pf1N1B4_285 0.76 -1.8 342,428 - Pf1N1B4_285 0.87 -1.8 342,428 - Pf1N1B4_285 0.87 -0.1 342,461 - -0.1 342,461 - -2.2 342,461 - -1.2 342,461 - -2.4 342,528 + -1.5 342,529 - -0.1 342,529 - -0.1 342,529 - -2.9 342,529 - -0.1
Or see this region's nucleotide sequence