Experiment: Nitrate reduction, replicate B t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2010 and Pf1N1B4_2011 are separated by 11 nucleotides Pf1N1B4_2011 and Pf1N1B4_2012 are separated by 284 nucleotides
Pf1N1B4_2010 - Pyruvate carboxyl transferase subunit A (EC 6.4.1.1), at 2,295,359 to 2,296,774
_2010
Pf1N1B4_2011 - Pyruvate carboxyl transferase subunit B (EC 6.4.1.1), at 2,296,786 to 2,298,594
_2011
Pf1N1B4_2012 - Amino acid ABC transporter, permease protein, at 2,298,879 to 2,299,661
_2012
Position (kb)
2296
2297
2298
2299 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2296.115 kb on + strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.116 kb on - strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.205 kb on + strand at 2296.205 kb on + strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand, within Pf1N1B4_2010 at 2296.206 kb on - strand at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.262 kb on - strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.427 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.469 kb on + strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.470 kb on - strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.496 kb on + strand, within Pf1N1B4_2010 at 2296.497 kb on - strand, within Pf1N1B4_2010 at 2296.613 kb on + strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.614 kb on - strand, within Pf1N1B4_2010 at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.700 kb on + strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.701 kb on - strand at 2296.779 kb on + strand at 2296.779 kb on + strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.780 kb on - strand at 2296.953 kb on - strand at 2296.953 kb on - strand at 2297.010 kb on + strand at 2297.011 kb on - strand, within Pf1N1B4_2011 at 2297.065 kb on + strand, within Pf1N1B4_2011 at 2297.157 kb on + strand, within Pf1N1B4_2011 at 2297.183 kb on + strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.184 kb on - strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.221 kb on + strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.222 kb on - strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.593 kb on + strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.594 kb on - strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.599 kb on + strand, within Pf1N1B4_2011 at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.600 kb on - strand at 2297.600 kb on - strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.617 kb on + strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011 at 2297.618 kb on - strand, within Pf1N1B4_2011 at 2297.861 kb on - strand, within Pf1N1B4_2011 at 2297.874 kb on + strand, within Pf1N1B4_2011 at 2297.874 kb on + strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2297.891 kb on - strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.067 kb on + strand, within Pf1N1B4_2011 at 2298.068 kb on - strand, within Pf1N1B4_2011 at 2298.068 kb on - strand, within Pf1N1B4_2011 at 2298.230 kb on - strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.283 kb on + strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.284 kb on - strand, within Pf1N1B4_2011 at 2298.572 kb on + strand at 2298.572 kb on + strand at 2298.572 kb on + strand at 2298.573 kb on - strand at 2298.573 kb on - strand at 2298.573 kb on - strand at 2298.597 kb on + strand at 2298.598 kb on - strand at 2298.611 kb on + strand at 2298.612 kb on - strand at 2298.612 kb on - strand at 2298.612 kb on - strand at 2298.619 kb on + strand at 2298.620 kb on - strand at 2298.717 kb on + strand at 2298.727 kb on + strand at 2298.727 kb on + strand at 2298.728 kb on - strand at 2298.742 kb on + strand at 2298.743 kb on - strand at 2298.743 kb on - strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.796 kb on + strand at 2298.797 kb on - strand at 2298.797 kb on - strand at 2298.797 kb on - strand at 2298.870 kb on + strand at 2298.870 kb on + strand at 2298.891 kb on + strand at 2298.891 kb on + strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.892 kb on - strand at 2298.904 kb on - strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.957 kb on + strand at 2298.958 kb on - strand, within Pf1N1B4_2012 at 2298.958 kb on - strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.961 kb on + strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.962 kb on - strand, within Pf1N1B4_2012 at 2298.988 kb on + strand, within Pf1N1B4_2012 at 2298.989 kb on - strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.990 kb on + strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2298.991 kb on - strand, within Pf1N1B4_2012 at 2299.012 kb on - strand, within Pf1N1B4_2012 at 2299.082 kb on - strand, within Pf1N1B4_2012 at 2299.249 kb on + strand, within Pf1N1B4_2012 at 2299.249 kb on + strand, within Pf1N1B4_2012 at 2299.250 kb on - strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.321 kb on + strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.322 kb on - strand, within Pf1N1B4_2012 at 2299.352 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.372 kb on + strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012 at 2299.373 kb on - strand, within Pf1N1B4_2012
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B t2 remove 2,296,115 + Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,116 - Pf1N1B4_2010 0.53 -0.0 2,296,205 + Pf1N1B4_2010 0.60 -0.0 2,296,205 + Pf1N1B4_2010 0.60 -0.0 2,296,205 + -0.0 2,296,205 + Pf1N1B4_2010 0.60 -3.4 2,296,206 - Pf1N1B4_2010 0.60 -0.0 2,296,206 - Pf1N1B4_2010 0.60 -3.0 2,296,206 - -0.0 2,296,262 - Pf1N1B4_2010 0.64 -4.4 2,296,262 - Pf1N1B4_2010 0.64 -1.3 2,296,427 + Pf1N1B4_2010 0.75 -0.0 2,296,427 + Pf1N1B4_2010 0.75 -0.0 2,296,469 + Pf1N1B4_2010 0.78 -0.0 2,296,469 + Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -0.0 2,296,470 - Pf1N1B4_2010 0.78 -4.3 2,296,496 + Pf1N1B4_2010 0.80 -2.8 2,296,496 + Pf1N1B4_2010 0.80 -2.2 2,296,496 + Pf1N1B4_2010 0.80 -0.0 2,296,497 - Pf1N1B4_2010 0.80 -0.0 2,296,613 + Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,614 - Pf1N1B4_2010 0.89 -0.0 2,296,700 + -0.5 2,296,700 + -0.0 2,296,700 + -0.0 2,296,700 + -4.1 2,296,700 + -0.0 2,296,700 + -0.0 2,296,701 - -0.0 2,296,701 - -0.0 2,296,701 - -0.0 2,296,779 + -0.0 2,296,779 + -3.5 2,296,780 - -4.3 2,296,780 - -0.0 2,296,780 - -0.0 2,296,780 - -0.0 2,296,780 - -4.5 2,296,953 - -0.0 2,296,953 - -0.0 2,297,010 + -2.4 2,297,011 - Pf1N1B4_2011 0.12 -0.0 2,297,065 + Pf1N1B4_2011 0.15 -0.0 2,297,157 + Pf1N1B4_2011 0.21 -2.5 2,297,183 + Pf1N1B4_2011 0.22 -0.0 2,297,184 - Pf1N1B4_2011 0.22 -0.0 2,297,184 - Pf1N1B4_2011 0.22 -3.9 2,297,221 + Pf1N1B4_2011 0.24 -0.0 2,297,221 + Pf1N1B4_2011 0.24 -0.0 2,297,222 - Pf1N1B4_2011 0.24 -0.0 2,297,222 - Pf1N1B4_2011 0.24 -3.8 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,593 + Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -5.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,594 - Pf1N1B4_2011 0.45 -0.0 2,297,599 + Pf1N1B4_2011 0.45 -0.0 2,297,599 + Pf1N1B4_2011 0.45 -0.0 2,297,600 - Pf1N1B4_2011 0.45 -1.3 2,297,600 - -0.0 2,297,600 - Pf1N1B4_2011 0.45 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -1.0 2,297,617 + Pf1N1B4_2011 0.46 -3.5 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,617 + Pf1N1B4_2011 0.46 -0.0 2,297,618 - Pf1N1B4_2011 0.46 -4.4 2,297,618 - Pf1N1B4_2011 0.46 -3.4 2,297,861 - Pf1N1B4_2011 0.59 -0.0 2,297,874 + Pf1N1B4_2011 0.60 -3.1 2,297,874 + Pf1N1B4_2011 0.60 -0.0 2,297,891 - Pf1N1B4_2011 0.61 -0.0 2,297,891 - Pf1N1B4_2011 0.61 -0.0 2,297,891 - Pf1N1B4_2011 0.61 -0.0 2,297,891 - Pf1N1B4_2011 0.61 -2.8 2,298,067 + Pf1N1B4_2011 0.71 -0.0 2,298,067 + Pf1N1B4_2011 0.71 -2.9 2,298,067 + Pf1N1B4_2011 0.71 -0.0 2,298,068 - Pf1N1B4_2011 0.71 -0.0 2,298,068 - Pf1N1B4_2011 0.71 -0.0 2,298,230 - Pf1N1B4_2011 0.80 -0.0 2,298,283 + Pf1N1B4_2011 0.83 -2.6 2,298,283 + Pf1N1B4_2011 0.83 -0.0 2,298,283 + Pf1N1B4_2011 0.83 -2.8 2,298,283 + Pf1N1B4_2011 0.83 -0.0 2,298,283 + Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,284 - Pf1N1B4_2011 0.83 -0.0 2,298,572 + -0.0 2,298,572 + -0.6 2,298,572 + -1.0 2,298,573 - -4.5 2,298,573 - -0.0 2,298,573 - -0.0 2,298,597 + -0.0 2,298,598 - -0.0 2,298,611 + -0.0 2,298,612 - -2.2 2,298,612 - -0.0 2,298,612 - -2.0 2,298,619 + -0.0 2,298,620 - -0.0 2,298,717 + -0.0 2,298,727 + -0.0 2,298,727 + -1.7 2,298,728 - -2.0 2,298,742 + -0.0 2,298,743 - -0.0 2,298,743 - -3.0 2,298,796 + -2.1 2,298,796 + -0.0 2,298,796 + -0.0 2,298,796 + -1.7 2,298,796 + -0.0 2,298,797 - -0.0 2,298,797 - -0.0 2,298,797 - -0.0 2,298,870 + -0.0 2,298,870 + +0.3 2,298,891 + -0.0 2,298,891 + -0.0 2,298,892 - -0.0 2,298,892 - -0.0 2,298,892 - -0.0 2,298,892 - -0.0 2,298,892 - -0.0 2,298,892 - -0.0 2,298,892 - -2.2 2,298,904 - -0.0 2,298,957 + -0.0 2,298,957 + -0.0 2,298,957 + -1.9 2,298,957 + -1.6 2,298,958 - Pf1N1B4_2012 0.10 -0.0 2,298,958 - Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,961 + Pf1N1B4_2012 0.10 -0.0 2,298,962 - Pf1N1B4_2012 0.11 -0.3 2,298,962 - Pf1N1B4_2012 0.11 -0.9 2,298,962 - Pf1N1B4_2012 0.11 +1.0 2,298,962 - Pf1N1B4_2012 0.11 -0.0 2,298,962 - Pf1N1B4_2012 0.11 -0.0 2,298,988 + Pf1N1B4_2012 0.14 -0.0 2,298,989 - Pf1N1B4_2012 0.14 -0.0 2,298,990 + Pf1N1B4_2012 0.14 -0.0 2,298,990 + Pf1N1B4_2012 0.14 -1.1 2,298,990 + Pf1N1B4_2012 0.14 -0.0 2,298,991 - Pf1N1B4_2012 0.14 -1.0 2,298,991 - Pf1N1B4_2012 0.14 -2.1 2,298,991 - Pf1N1B4_2012 0.14 -2.0 2,298,991 - Pf1N1B4_2012 0.14 -0.0 2,298,991 - Pf1N1B4_2012 0.14 -2.2 2,299,012 - Pf1N1B4_2012 0.17 -0.0 2,299,082 - Pf1N1B4_2012 0.26 -0.0 2,299,249 + Pf1N1B4_2012 0.47 -0.2 2,299,249 + Pf1N1B4_2012 0.47 -0.0 2,299,250 - Pf1N1B4_2012 0.47 -0.0 2,299,321 + Pf1N1B4_2012 0.56 -0.0 2,299,321 + Pf1N1B4_2012 0.56 -0.0 2,299,321 + Pf1N1B4_2012 0.56 -0.0 2,299,322 - Pf1N1B4_2012 0.57 -0.0 2,299,322 - Pf1N1B4_2012 0.57 -0.0 2,299,322 - Pf1N1B4_2012 0.57 -0.0 2,299,352 + Pf1N1B4_2012 0.60 -3.5 2,299,372 + Pf1N1B4_2012 0.63 -0.0 2,299,372 + Pf1N1B4_2012 0.63 -0.0 2,299,372 + Pf1N1B4_2012 0.63 -1.4 2,299,372 + Pf1N1B4_2012 0.63 -0.0 2,299,372 + Pf1N1B4_2012 0.63 -0.0 2,299,372 + Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -2.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0 2,299,373 - Pf1N1B4_2012 0.63 -0.0
Or see this region's nucleotide sequence