Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0105 and Psyr_0106 are separated by 56 nucleotides Psyr_0106 and Psyr_0107 are separated by 201 nucleotides Psyr_0107 and Psyr_0108 are separated by 210 nucleotides
Psyr_0105: Psyr_0105 - Integrase, catalytic region, at 116,991 to 117,812
_0105
Psyr_0106: Psyr_0106 - Transposase IS3/IS911, at 117,869 to 118,168
_0106
Psyr_0107: Psyr_0107 - hypothetical protein, at 118,370 to 118,735
_0107
Psyr_0108: Psyr_0108 - Major facilitator superfamily, at 118,946 to 120,304
_0108
Position (kb)
118
119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.207 kb on + strand at 118.235 kb on - strand at 118.249 kb on + strand at 118.282 kb on + strand at 118.283 kb on - strand at 118.283 kb on - strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.320 kb on - strand at 118.320 kb on - strand at 118.320 kb on - strand at 118.519 kb on + strand, within Psyr_0107 at 118.520 kb on - strand, within Psyr_0107 at 118.605 kb on + strand, within Psyr_0107 at 118.606 kb on - strand, within Psyr_0107 at 118.609 kb on + strand, within Psyr_0107 at 118.609 kb on + strand, within Psyr_0107 at 118.610 kb on - strand, within Psyr_0107 at 118.666 kb on + strand, within Psyr_0107 at 118.666 kb on + strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.776 kb on + strand at 118.823 kb on + strand at 118.824 kb on - strand at 118.824 kb on - strand at 118.981 kb on + strand at 119.064 kb on + strand at 119.065 kb on - strand at 119.065 kb on - strand at 119.075 kb on + strand at 119.141 kb on + strand, within Psyr_0108 at 119.141 kb on + strand, within Psyr_0108 at 119.141 kb on + strand, within Psyr_0108 at 119.159 kb on - strand, within Psyr_0108 at 119.192 kb on - strand, within Psyr_0108 at 119.285 kb on + strand, within Psyr_0108 at 119.285 kb on + strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.343 kb on - strand, within Psyr_0108
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 118,207 + +1.0 118,235 - +1.8 118,249 + -1.1 118,282 + -0.1 118,283 - +0.3 118,283 - -0.7 118,292 + -0.3 118,292 + +1.2 118,292 + -0.7 118,292 + -0.5 118,292 + +0.4 118,292 + +0.8 118,292 + +0.7 118,292 + +0.3 118,293 - +1.0 118,293 - -2.1 118,293 - -1.5 118,293 - +1.1 118,293 - +1.5 118,293 - +0.4 118,296 + -2.4 118,296 + +1.2 118,296 + -1.5 118,296 + -1.4 118,296 + -1.2 118,297 - -0.7 118,297 - +0.7 118,297 - -1.5 118,297 - +0.2 118,297 - -1.5 118,297 - +0.5 118,297 - -0.1 118,319 + +0.3 118,319 + +0.1 118,319 + +0.1 118,319 + +0.4 118,320 - +1.6 118,320 - -0.2 118,320 - +0.0 118,519 + Psyr_0107 0.41 -1.3 118,520 - Psyr_0107 0.41 -0.4 118,605 + Psyr_0107 0.64 +0.3 118,606 - Psyr_0107 0.64 -0.3 118,609 + Psyr_0107 0.65 -1.2 118,609 + Psyr_0107 0.65 +0.1 118,610 - Psyr_0107 0.66 -0.3 118,666 + Psyr_0107 0.81 -0.6 118,666 + Psyr_0107 0.81 -1.0 118,667 - Psyr_0107 0.81 -1.9 118,667 - Psyr_0107 0.81 +0.5 118,667 - Psyr_0107 0.81 -1.8 118,667 - Psyr_0107 0.81 +0.8 118,667 - Psyr_0107 0.81 -0.2 118,667 - Psyr_0107 0.81 -0.1 118,667 - Psyr_0107 0.81 -1.3 118,678 + Psyr_0107 0.84 -0.6 118,678 + Psyr_0107 0.84 +2.1 118,678 + Psyr_0107 0.84 +0.7 118,679 - Psyr_0107 0.84 -1.0 118,679 - Psyr_0107 0.84 -1.1 118,679 - Psyr_0107 0.84 +1.2 118,679 - Psyr_0107 0.84 -0.8 118,679 - Psyr_0107 0.84 +2.4 118,679 - Psyr_0107 0.84 +0.0 118,776 + -1.1 118,823 + -0.3 118,824 - -0.4 118,824 - +0.3 118,981 + +0.7 119,064 + -1.0 119,065 - +1.1 119,065 - -0.6 119,075 + -1.2 119,141 + Psyr_0108 0.14 -1.9 119,141 + Psyr_0108 0.14 -0.5 119,141 + Psyr_0108 0.14 -0.1 119,159 - Psyr_0108 0.16 -0.5 119,192 - Psyr_0108 0.18 -0.2 119,285 + Psyr_0108 0.25 -1.6 119,285 + Psyr_0108 0.25 +1.1 119,286 - Psyr_0108 0.25 -1.3 119,286 - Psyr_0108 0.25 +0.6 119,286 - Psyr_0108 0.25 -0.6 119,286 - Psyr_0108 0.25 +1.1 119,286 - Psyr_0108 0.25 -1.8 119,306 + Psyr_0108 0.26 -1.2 119,306 + Psyr_0108 0.26 +0.2 119,306 + Psyr_0108 0.26 -0.6 119,306 + Psyr_0108 0.26 -0.4 119,306 + Psyr_0108 0.26 +0.4 119,306 + Psyr_0108 0.26 +0.1 119,307 - Psyr_0108 0.27 +1.1 119,307 - Psyr_0108 0.27 +0.5 119,307 - Psyr_0108 0.27 +1.0 119,307 - Psyr_0108 0.27 -0.2 119,307 - Psyr_0108 0.27 -0.2 119,307 - Psyr_0108 0.27 -2.0 119,307 - Psyr_0108 0.27 +0.4 119,343 - Psyr_0108 0.29 +0.5
Or see this region's nucleotide sequence