Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2895

Experiment: library passed through aphids in food, 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2894 and Psyr_RNA45 are separated by 91 nucleotidesPsyr_RNA45 and Psyr_2895 are separated by 66 nucleotidesPsyr_2895 and Psyr_2896 are separated by 32 nucleotides Psyr_2894: Psyr_2894 - conserved hypothetical protein, at 3,473,200 to 3,473,658 _2894 Psyr_RNA45: Psyr_RNA45 - tRNA-Gly, at 3,473,750 to 3,473,825 _RNA45 Psyr_2895: Psyr_2895 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,473,892 to 3,474,452 _2895 Psyr_2896: Psyr_2896 - Excinuclease ABC subunit C, at 3,474,485 to 3,476,308 _2896 Position (kb) 3473 3474 3475Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 3473.072 kb on + strandat 3473.073 kb on - strandat 3473.073 kb on - strandat 3473.073 kb on - strandat 3473.083 kb on + strandat 3473.083 kb on + strandat 3473.084 kb on - strandat 3473.105 kb on + strandat 3473.166 kb on + strandat 3473.166 kb on + strandat 3473.166 kb on + strandat 3473.167 kb on - strandat 3473.193 kb on - strandat 3473.203 kb on + strandat 3473.203 kb on + strandat 3473.234 kb on - strandat 3473.545 kb on + strand, within Psyr_2894at 3473.546 kb on - strand, within Psyr_2894at 3473.656 kb on + strandat 3473.656 kb on + strandat 3473.657 kb on - strandat 3473.657 kb on - strandat 3474.508 kb on - strandat 3474.508 kb on - strandat 3474.733 kb on + strand, within Psyr_2896at 3474.793 kb on - strand, within Psyr_2896at 3474.863 kb on + strand, within Psyr_2896at 3474.964 kb on - strand, within Psyr_2896at 3474.964 kb on - strand, within Psyr_2896at 3474.996 kb on + strand, within Psyr_2896at 3475.119 kb on + strand, within Psyr_2896at 3475.405 kb on - strand, within Psyr_2896at 3475.448 kb on + strand, within Psyr_2896at 3475.448 kb on + strand, within Psyr_2896at 3475.449 kb on - strand, within Psyr_2896at 3475.449 kb on - strand, within Psyr_2896at 3475.449 kb on - strand, within Psyr_2896

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours
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3,473,072 + -1.3
3,473,073 - -0.5
3,473,073 - -0.1
3,473,073 - +0.7
3,473,083 + +1.6
3,473,083 + -0.5
3,473,084 - +3.3
3,473,105 + -0.6
3,473,166 + -1.6
3,473,166 + +0.5
3,473,166 + +0.1
3,473,167 - +1.3
3,473,193 - -0.6
3,473,203 + -2.3
3,473,203 + +6.2
3,473,234 - +1.5
3,473,545 + Psyr_2894 0.75 -1.2
3,473,546 - Psyr_2894 0.75 +1.5
3,473,656 + +0.4
3,473,656 + -0.1
3,473,657 - +1.9
3,473,657 - -1.4
3,474,508 - -3.7
3,474,508 - -0.6
3,474,733 + Psyr_2896 0.14 -1.5
3,474,793 - Psyr_2896 0.17 -1.4
3,474,863 + Psyr_2896 0.21 -1.4
3,474,964 - Psyr_2896 0.26 -0.3
3,474,964 - Psyr_2896 0.26 -0.3
3,474,996 + Psyr_2896 0.28 +0.5
3,475,119 + Psyr_2896 0.35 +0.0
3,475,405 - Psyr_2896 0.50 +0.2
3,475,448 + Psyr_2896 0.53 +0.3
3,475,448 + Psyr_2896 0.53 -1.0
3,475,449 - Psyr_2896 0.53 -0.2
3,475,449 - Psyr_2896 0.53 -0.3
3,475,449 - Psyr_2896 0.53 +1.2

Or see this region's nucleotide sequence