Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0298 and Psyr_0299 are separated by 250 nucleotides Psyr_0299 and Psyr_0300 are separated by 121 nucleotides Psyr_0300 and Psyr_0301 are separated by 231 nucleotides
Psyr_0298: Psyr_0298 - amino acid ABC transporter substrate-binding protein, PAAT family, at 317,876 to 318,661
_0298
Psyr_0299: Psyr_0299 - transcriptional regulator, GntR family, at 318,912 to 319,616
_0299
Psyr_0300: Psyr_0300 - Thioredoxin, at 319,738 to 320,088
_0300
Psyr_0301: Psyr_0301 - transcription termination factor Rho, at 320,320 to 321,579
_0301
Position (kb) 318
319
320 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 317.933 kb on - strand at 318.009 kb on + strand, within Psyr_0298 at 318.009 kb on + strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.010 kb on - strand, within Psyr_0298 at 318.126 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.232 kb on + strand, within Psyr_0298 at 318.233 kb on - strand, within Psyr_0298 at 318.233 kb on - strand, within Psyr_0298 at 318.297 kb on + strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.298 kb on - strand, within Psyr_0298 at 318.309 kb on + strand, within Psyr_0298 at 318.472 kb on + strand, within Psyr_0298 at 318.635 kb on - strand at 318.635 kb on - strand at 318.661 kb on + strand at 318.661 kb on + strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.662 kb on - strand at 318.762 kb on + strand at 318.763 kb on - strand at 318.818 kb on + strand at 318.819 kb on - strand at 318.874 kb on - strand at 318.984 kb on + strand, within Psyr_0299 at 318.985 kb on - strand, within Psyr_0299 at 319.173 kb on + strand, within Psyr_0299 at 319.173 kb on + strand, within Psyr_0299 at 319.174 kb on - strand, within Psyr_0299 at 319.174 kb on - strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.279 kb on + strand, within Psyr_0299 at 319.562 kb on + strand at 319.562 kb on + strand at 319.563 kb on - strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.620 kb on - strand at 319.620 kb on - strand at 319.627 kb on + strand at 319.644 kb on + strand at 319.645 kb on - strand at 319.645 kb on - strand at 319.645 kb on - strand at 319.645 kb on - strand at 319.681 kb on + strand at 319.719 kb on + strand at 320.076 kb on + strand at 320.135 kb on - strand at 320.137 kb on + strand at 320.160 kb on + strand at 320.228 kb on + strand at 320.231 kb on + strand at 320.282 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.297 kb on + strand at 320.305 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 317,933 - -1.6 318,009 + Psyr_0298 0.17 +0.6 318,009 + Psyr_0298 0.17 +1.5 318,010 - Psyr_0298 0.17 -1.2 318,010 - Psyr_0298 0.17 +0.5 318,010 - Psyr_0298 0.17 +2.5 318,010 - Psyr_0298 0.17 +0.4 318,010 - Psyr_0298 0.17 -0.1 318,010 - Psyr_0298 0.17 -1.5 318,010 - Psyr_0298 0.17 -0.7 318,126 + Psyr_0298 0.32 +0.3 318,232 + Psyr_0298 0.45 +0.5 318,232 + Psyr_0298 0.45 +2.8 318,232 + Psyr_0298 0.45 -0.7 318,233 - Psyr_0298 0.45 +1.6 318,233 - Psyr_0298 0.45 +0.0 318,297 + Psyr_0298 0.54 -0.1 318,298 - Psyr_0298 0.54 -0.8 318,298 - Psyr_0298 0.54 -0.1 318,298 - Psyr_0298 0.54 -1.8 318,298 - Psyr_0298 0.54 -0.1 318,309 + Psyr_0298 0.55 -0.2 318,472 + Psyr_0298 0.76 -0.4 318,635 - -0.6 318,635 - +2.5 318,661 + -2.0 318,661 + +0.6 318,662 - +1.5 318,662 - -0.5 318,662 - +0.8 318,662 - +0.4 318,762 + +0.3 318,763 - -0.9 318,818 + +0.8 318,819 - +0.7 318,874 - +0.4 318,984 + Psyr_0299 0.10 +0.9 318,985 - Psyr_0299 0.10 +1.2 319,173 + Psyr_0299 0.37 -0.4 319,173 + Psyr_0299 0.37 +0.7 319,174 - Psyr_0299 0.37 -0.6 319,174 - Psyr_0299 0.37 +2.7 319,279 + Psyr_0299 0.52 -0.3 319,279 + Psyr_0299 0.52 +0.3 319,279 + Psyr_0299 0.52 +0.8 319,279 + Psyr_0299 0.52 -0.9 319,279 + Psyr_0299 0.52 +0.5 319,279 + Psyr_0299 0.52 +2.3 319,562 + +0.3 319,562 + +0.9 319,563 - -0.1 319,619 + -1.9 319,619 + +0.3 319,619 + -0.5 319,619 + -2.7 319,619 + +1.2 319,620 - -0.4 319,620 - +0.8 319,627 + -1.2 319,644 + +0.1 319,645 - -0.6 319,645 - +0.4 319,645 - -1.2 319,645 - +0.1 319,681 + +0.9 319,719 + -1.7 320,076 + +1.4 320,135 - -0.5 320,137 + -0.9 320,160 + -1.1 320,228 + -0.5 320,231 + -0.9 320,282 + -0.7 320,297 + -1.1 320,297 + -0.3 320,297 + -0.1 320,297 + -2.2 320,297 + -0.8 320,297 + +0.3 320,297 + +1.1 320,297 + +2.0 320,305 + -0.3
Or see this region's nucleotide sequence