Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25930

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25915 and BWI76_RS25920 are separated by 59 nucleotidesBWI76_RS25920 and BWI76_RS25925 are separated by 94 nucleotidesBWI76_RS25925 and BWI76_RS25930 are separated by 11 nucleotidesBWI76_RS25930 and BWI76_RS25935 overlap by 14 nucleotidesBWI76_RS25935 and BWI76_RS25940 overlap by 80 nucleotidesBWI76_RS25940 and BWI76_RS25945 are separated by 21 nucleotides BWI76_RS25915: BWI76_RS25915 - protein SlyX, at 5,359,169 to 5,359,387 _RS25915 BWI76_RS25920: BWI76_RS25920 - peptidylprolyl isomerase, at 5,359,447 to 5,360,034 _RS25920 BWI76_RS25925: BWI76_RS25925 - hypothetical protein, at 5,360,129 to 5,360,329 _RS25925 BWI76_RS25930: BWI76_RS25930 - glutathione-regulated potassium-efflux system protein KefB, at 5,360,341 to 5,362,146 _RS25930 BWI76_RS25935: BWI76_RS25935 - glutathione-regulated potassium-efflux system ancillary protein KefG, at 5,362,133 to 5,362,693 _RS25935 BWI76_RS25940: BWI76_RS25940 - hypothetical protein, at 5,362,614 to 5,362,847 _RS25940 BWI76_RS25945: BWI76_RS25945 - ABC transporter ATP-binding protein, at 5,362,869 to 5,364,773 _RS25945 Position (kb) 5360 5361 5362 5363Strain fitness (log2 ratio) -1 0 1at 5359.521 kb on - strand, within BWI76_RS25920at 5359.585 kb on + strand, within BWI76_RS25920at 5359.835 kb on - strand, within BWI76_RS25920at 5359.993 kb on - strandat 5360.185 kb on + strand, within BWI76_RS25925at 5360.254 kb on - strand, within BWI76_RS25925at 5360.278 kb on - strand, within BWI76_RS25925at 5360.425 kb on - strandat 5360.450 kb on - strandat 5360.628 kb on + strand, within BWI76_RS25930at 5360.703 kb on - strand, within BWI76_RS25930at 5361.199 kb on + strand, within BWI76_RS25930at 5361.259 kb on + strand, within BWI76_RS25930at 5361.267 kb on - strand, within BWI76_RS25930at 5361.389 kb on + strand, within BWI76_RS25930at 5361.407 kb on - strand, within BWI76_RS25930at 5361.478 kb on + strand, within BWI76_RS25930at 5361.493 kb on + strand, within BWI76_RS25930at 5361.542 kb on - strand, within BWI76_RS25930at 5361.692 kb on + strand, within BWI76_RS25930at 5361.706 kb on - strand, within BWI76_RS25930at 5361.724 kb on + strand, within BWI76_RS25930at 5361.741 kb on - strand, within BWI76_RS25930at 5361.932 kb on - strand, within BWI76_RS25930at 5362.003 kb on + strandat 5362.211 kb on + strand, within BWI76_RS25935at 5362.438 kb on - strand, within BWI76_RS25935at 5362.480 kb on - strand, within BWI76_RS25935at 5362.480 kb on - strand, within BWI76_RS25935at 5362.927 kb on - strandat 5362.937 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x; and D-Glucose 99.9 mM
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5,359,521 - BWI76_RS25920 0.13 -0.4
5,359,585 + BWI76_RS25920 0.23 +0.6
5,359,835 - BWI76_RS25920 0.66 +0.1
5,359,993 - -0.4
5,360,185 + BWI76_RS25925 0.28 -0.3
5,360,254 - BWI76_RS25925 0.62 +0.1
5,360,278 - BWI76_RS25925 0.74 +0.0
5,360,425 - +1.0
5,360,450 - -0.2
5,360,628 + BWI76_RS25930 0.16 +0.1
5,360,703 - BWI76_RS25930 0.20 +0.1
5,361,199 + BWI76_RS25930 0.48 -0.0
5,361,259 + BWI76_RS25930 0.51 +0.2
5,361,267 - BWI76_RS25930 0.51 +0.3
5,361,389 + BWI76_RS25930 0.58 +0.3
5,361,407 - BWI76_RS25930 0.59 -0.1
5,361,478 + BWI76_RS25930 0.63 +1.5
5,361,493 + BWI76_RS25930 0.64 +0.9
5,361,542 - BWI76_RS25930 0.67 +1.4
5,361,692 + BWI76_RS25930 0.75 +0.2
5,361,706 - BWI76_RS25930 0.76 +0.0
5,361,724 + BWI76_RS25930 0.77 -0.0
5,361,741 - BWI76_RS25930 0.78 -0.2
5,361,932 - BWI76_RS25930 0.88 -0.1
5,362,003 + +0.7
5,362,211 + BWI76_RS25935 0.14 -0.4
5,362,438 - BWI76_RS25935 0.54 +0.5
5,362,480 - BWI76_RS25935 0.62 -0.3
5,362,480 - BWI76_RS25935 0.62 -0.6
5,362,927 - -0.7
5,362,937 + -1.5

Or see this region's nucleotide sequence