Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4159 and GFF4160 are separated by 4 nucleotides GFF4160 and GFF4161 are separated by 165 nucleotides GFF4161 and GFF4162 are separated by 148 nucleotides GFF4162 and GFF4163 are separated by 85 nucleotides
GFF4159 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 51,250 to 51,639
GFF4159
GFF4160 - UPF0125 protein yfjF, at 51,644 to 51,919
GFF4160
GFF4161 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 52,085 to 52,423
GFF4161
GFF4162 - DNA repair protein RecN, at 52,572 to 54,233
GFF4162
GFF4163 - NAD kinase (EC 2.7.1.23), at 54,319 to 55,197
GFF4163
Position (kb) 52
53
54
55 Strain fitness (log2 ratio) -1
0
1 at 51.680 kb on + strand, within GFF4160 at 51.781 kb on + strand, within GFF4160 at 51.786 kb on + strand, within GFF4160 at 51.787 kb on - strand, within GFF4160 at 51.794 kb on + strand, within GFF4160 at 51.882 kb on + strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.913 kb on + strand at 52.060 kb on - strand at 52.087 kb on - strand at 52.200 kb on + strand, within GFF4161 at 52.201 kb on - strand, within GFF4161 at 52.216 kb on + strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.304 kb on - strand, within GFF4161 at 52.310 kb on - strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.314 kb on + strand, within GFF4161 at 52.467 kb on - strand at 52.470 kb on - strand at 52.470 kb on - strand at 52.470 kb on - strand at 52.473 kb on - strand at 52.487 kb on - strand at 52.504 kb on - strand at 53.180 kb on + strand, within GFF4162 at 53.497 kb on - strand, within GFF4162 at 53.634 kb on - strand, within GFF4162 at 53.789 kb on - strand, within GFF4162 at 53.789 kb on - strand, within GFF4162 at 53.916 kb on - strand, within GFF4162 at 54.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 51,680 + GFF4160 0.13 -0.8 51,781 + GFF4160 0.50 -0.2 51,786 + GFF4160 0.51 -0.2 51,787 - GFF4160 0.52 +1.3 51,794 + GFF4160 0.54 +0.1 51,882 + GFF4160 0.86 -0.0 51,883 - GFF4160 0.87 +0.3 51,883 - GFF4160 0.87 +0.0 51,913 + +0.5 52,060 - -0.1 52,087 - +0.2 52,200 + GFF4161 0.34 -0.6 52,201 - GFF4161 0.34 +0.9 52,216 + GFF4161 0.39 +0.2 52,304 - GFF4161 0.65 +0.4 52,304 - GFF4161 0.65 +0.4 52,304 - GFF4161 0.65 +0.1 52,310 - GFF4161 0.66 -0.1 52,314 + GFF4161 0.68 +0.7 52,314 + GFF4161 0.68 +0.5 52,314 + GFF4161 0.68 -0.0 52,467 - +0.1 52,470 - -1.6 52,470 - +0.1 52,470 - +0.3 52,473 - -1.1 52,487 - -0.3 52,504 - -0.1 53,180 + GFF4162 0.37 -0.9 53,497 - GFF4162 0.56 -1.0 53,634 - GFF4162 0.64 +0.3 53,789 - GFF4162 0.73 +0.1 53,789 - GFF4162 0.73 -0.2 53,916 - GFF4162 0.81 +0.6 54,132 - -0.2
Or see this region's nucleotide sequence