Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4064 and GFF4065 overlap by 4 nucleotides GFF4065 and GFF4066 are separated by 4 nucleotides
GFF4064 - Uncharacterized protein ImpJ/VasE, at 25,181 to 26,524
GFF4064
GFF4065 - Outer membrane protein ImpK/VasF, OmpA/MotB domain, at 26,521 to 27,825
GFF4065
GFF4066 - Putative inner membrane protein, at 27,830 to 28,603
GFF4066
Position (kb)
26
27
28 Strain fitness (log2 ratio)
-1
0
1
2 at 25.611 kb on + strand, within GFF4064 at 25.724 kb on - strand, within GFF4064 at 25.753 kb on + strand, within GFF4064 at 25.754 kb on - strand, within GFF4064 at 25.780 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.009 kb on + strand, within GFF4064 at 26.010 kb on - strand, within GFF4064 at 26.043 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.052 kb on - strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.165 kb on + strand, within GFF4064 at 26.399 kb on + strand at 26.399 kb on + strand at 26.400 kb on - strand at 26.400 kb on - strand at 26.402 kb on + strand at 26.469 kb on - strand at 26.589 kb on + strand at 26.831 kb on + strand, within GFF4065 at 26.832 kb on - strand, within GFF4065 at 26.873 kb on + strand, within GFF4065 at 26.889 kb on + strand, within GFF4065 at 27.051 kb on - strand, within GFF4065 at 27.060 kb on + strand, within GFF4065 at 27.065 kb on - strand, within GFF4065 at 27.136 kb on - strand, within GFF4065 at 27.307 kb on + strand, within GFF4065 at 27.351 kb on + strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.352 kb on - strand, within GFF4065 at 27.478 kb on - strand, within GFF4065 at 27.656 kb on + strand, within GFF4065 at 27.874 kb on - strand at 27.914 kb on + strand, within GFF4066 at 27.921 kb on + strand, within GFF4066 at 27.922 kb on - strand, within GFF4066 at 27.954 kb on + strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 27.955 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.037 kb on - strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.100 kb on + strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.101 kb on - strand, within GFF4066 at 28.165 kb on + strand, within GFF4066 at 28.166 kb on - strand, within GFF4066 at 28.167 kb on + strand, within GFF4066 at 28.168 kb on - strand, within GFF4066 at 28.174 kb on - strand, within GFF4066 at 28.240 kb on - strand, within GFF4066 at 28.402 kb on + strand, within GFF4066 at 28.421 kb on + strand, within GFF4066 at 28.509 kb on - strand, within GFF4066 at 28.654 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 25,611 + GFF4064 0.32 -0.0 25,724 - GFF4064 0.40 +0.0 25,753 + GFF4064 0.43 -0.4 25,754 - GFF4064 0.43 -0.2 25,780 + GFF4064 0.45 +0.5 26,009 + GFF4064 0.62 +0.1 26,009 + GFF4064 0.62 +1.1 26,009 + GFF4064 0.62 +0.7 26,010 - GFF4064 0.62 -1.5 26,043 - GFF4064 0.64 -0.8 26,052 - GFF4064 0.65 -0.2 26,052 - GFF4064 0.65 +0.3 26,165 + GFF4064 0.73 +0.1 26,165 + GFF4064 0.73 -0.1 26,399 + +0.2 26,399 + -0.1 26,400 - +0.3 26,400 - +0.3 26,402 + +0.1 26,469 - +0.4 26,589 + -0.0 26,831 + GFF4065 0.24 +1.8 26,832 - GFF4065 0.24 -0.1 26,873 + GFF4065 0.27 +0.4 26,889 + GFF4065 0.28 -0.5 27,051 - GFF4065 0.41 -0.4 27,060 + GFF4065 0.41 -0.2 27,065 - GFF4065 0.42 -0.1 27,136 - GFF4065 0.47 -1.2 27,307 + GFF4065 0.60 +0.6 27,351 + GFF4065 0.64 -0.1 27,352 - GFF4065 0.64 -0.0 27,352 - GFF4065 0.64 -0.3 27,478 - GFF4065 0.73 -0.2 27,656 + GFF4065 0.87 -1.1 27,874 - +0.1 27,914 + GFF4066 0.11 +0.7 27,921 + GFF4066 0.12 +0.1 27,922 - GFF4066 0.12 +0.5 27,954 + GFF4066 0.16 +0.1 27,955 - GFF4066 0.16 +0.2 27,955 - GFF4066 0.16 -0.1 27,955 - GFF4066 0.16 +0.0 28,037 - GFF4066 0.27 -0.4 28,037 - GFF4066 0.27 -0.0 28,100 + GFF4066 0.35 +0.2 28,100 + GFF4066 0.35 +0.3 28,100 + GFF4066 0.35 +0.4 28,101 - GFF4066 0.35 +0.5 28,101 - GFF4066 0.35 -0.2 28,101 - GFF4066 0.35 +1.8 28,165 + GFF4066 0.43 +0.0 28,166 - GFF4066 0.43 -0.4 28,167 + GFF4066 0.44 +0.2 28,168 - GFF4066 0.44 -0.1 28,174 - GFF4066 0.44 +1.6 28,240 - GFF4066 0.53 -0.4 28,402 + GFF4066 0.74 +0.7 28,421 + GFF4066 0.76 -0.8 28,509 - GFF4066 0.88 +0.3 28,654 - -0.0
Or see this region's nucleotide sequence