Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF924 and GFF925 overlap by 6 nucleotides GFF925 and GFF926 are separated by 224 nucleotides GFF926 and GFF927 are separated by 115 nucleotides GFF927 and GFF928 are separated by 74 nucleotides
GFF924 - Trehalose operon transcriptional repressor, at 101,709 to 102,656
GFF924
GFF925 - hypothetical protein, at 102,651 to 102,767
GFF925
GFF926 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 102,992 to 105,748
GFF926
GFF927 - Mobile element protein, at 105,864 to 106,310
GFF927
GFF928 - Endoribonuclease L-PSP, at 106,385 to 106,771
GFF928
Position (kb)
102
103
104
105
106 Strain fitness (log2 ratio)
-2
-1
0
1 at 101.992 kb on + strand, within GFF924 at 102.064 kb on + strand, within GFF924 at 102.065 kb on - strand, within GFF924 at 102.123 kb on - strand, within GFF924 at 102.165 kb on - strand, within GFF924 at 102.337 kb on - strand, within GFF924 at 102.368 kb on + strand, within GFF924 at 102.369 kb on - strand, within GFF924 at 102.372 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.380 kb on - strand, within GFF924 at 102.458 kb on + strand, within GFF924 at 102.750 kb on - strand, within GFF925 at 102.764 kb on - strand at 103.035 kb on + strand at 103.040 kb on - strand at 103.040 kb on - strand at 103.243 kb on - strand at 103.336 kb on - strand, within GFF926 at 103.603 kb on + strand, within GFF926 at 103.606 kb on + strand, within GFF926 at 103.774 kb on + strand, within GFF926 at 103.906 kb on + strand, within GFF926 at 103.918 kb on + strand, within GFF926 at 103.919 kb on - strand, within GFF926 at 103.967 kb on - strand, within GFF926 at 103.977 kb on - strand, within GFF926 at 104.155 kb on + strand, within GFF926 at 104.248 kb on - strand, within GFF926 at 104.248 kb on - strand, within GFF926 at 104.486 kb on - strand, within GFF926 at 104.510 kb on - strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.530 kb on + strand, within GFF926 at 104.607 kb on - strand, within GFF926 at 104.669 kb on + strand, within GFF926 at 104.740 kb on + strand, within GFF926 at 104.847 kb on + strand, within GFF926 at 104.847 kb on + strand, within GFF926 at 104.915 kb on - strand, within GFF926 at 105.016 kb on + strand, within GFF926 at 105.256 kb on - strand, within GFF926 at 105.260 kb on + strand, within GFF926 at 105.293 kb on - strand, within GFF926 at 105.626 kb on + strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.657 kb on - strand at 105.690 kb on - strand at 105.691 kb on + strand at 105.691 kb on + strand at 105.691 kb on + strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.692 kb on - strand at 105.900 kb on - strand at 106.021 kb on + strand, within GFF927 at 106.025 kb on + strand, within GFF927 at 106.039 kb on - strand, within GFF927 at 106.039 kb on - strand, within GFF927 at 106.042 kb on + strand, within GFF927 at 106.073 kb on + strand, within GFF927 at 106.074 kb on - strand, within GFF927 at 106.083 kb on - strand, within GFF927 at 106.095 kb on + strand, within GFF927 at 106.144 kb on - strand, within GFF927 at 106.153 kb on - strand, within GFF927 at 106.164 kb on + strand, within GFF927 at 106.164 kb on + strand, within GFF927 at 106.501 kb on - strand, within GFF928 at 106.501 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.545 kb on - strand, within GFF928 at 106.595 kb on - strand, within GFF928 at 106.700 kb on - strand, within GFF928 at 106.718 kb on + strand, within GFF928 at 106.719 kb on - strand, within GFF928 at 106.722 kb on + strand, within GFF928 at 106.723 kb on - strand, within GFF928 at 106.723 kb on - strand, within GFF928
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 101,992 + GFF924 0.30 -0.0 102,064 + GFF924 0.37 +0.0 102,065 - GFF924 0.38 +0.1 102,123 - GFF924 0.44 -0.3 102,165 - GFF924 0.48 +1.5 102,337 - GFF924 0.66 +0.6 102,368 + GFF924 0.70 +0.6 102,369 - GFF924 0.70 +0.2 102,372 - GFF924 0.70 +0.0 102,380 - GFF924 0.71 -0.3 102,380 - GFF924 0.71 +0.3 102,458 + GFF924 0.79 +0.0 102,750 - GFF925 0.85 +0.2 102,764 - -0.7 103,035 + +0.5 103,040 - -0.5 103,040 - +0.3 103,243 - +0.3 103,336 - GFF926 0.12 -0.0 103,603 + GFF926 0.22 +0.3 103,606 + GFF926 0.22 +0.4 103,774 + GFF926 0.28 +0.7 103,906 + GFF926 0.33 +0.9 103,918 + GFF926 0.34 +0.5 103,919 - GFF926 0.34 +1.7 103,967 - GFF926 0.35 +0.2 103,977 - GFF926 0.36 +0.9 104,155 + GFF926 0.42 +0.4 104,248 - GFF926 0.46 +0.6 104,248 - GFF926 0.46 -0.0 104,486 - GFF926 0.54 +0.4 104,510 - GFF926 0.55 +0.5 104,530 + GFF926 0.56 +1.4 104,530 + GFF926 0.56 +0.7 104,530 + GFF926 0.56 -0.3 104,607 - GFF926 0.59 +1.4 104,669 + GFF926 0.61 +0.3 104,740 + GFF926 0.63 +0.3 104,847 + GFF926 0.67 +0.5 104,847 + GFF926 0.67 +0.2 104,915 - GFF926 0.70 +0.4 105,016 + GFF926 0.73 -0.1 105,256 - GFF926 0.82 -0.2 105,260 + GFF926 0.82 +0.2 105,293 - GFF926 0.83 -1.6 105,626 + -0.2 105,657 - -1.6 105,657 - +0.1 105,657 - +0.6 105,657 - -0.4 105,690 - +0.5 105,691 + -0.9 105,691 + +0.1 105,691 + +0.3 105,692 - +0.3 105,692 - +0.0 105,692 - +1.7 105,692 - +0.3 105,900 - +0.5 106,021 + GFF927 0.35 -0.2 106,025 + GFF927 0.36 -2.4 106,039 - GFF927 0.39 +0.0 106,039 - GFF927 0.39 +1.2 106,042 + GFF927 0.40 -0.2 106,073 + GFF927 0.47 -0.1 106,074 - GFF927 0.47 -0.4 106,083 - GFF927 0.49 -0.5 106,095 + GFF927 0.52 +0.1 106,144 - GFF927 0.63 -0.1 106,153 - GFF927 0.65 +0.7 106,164 + GFF927 0.67 -0.1 106,164 + GFF927 0.67 +0.6 106,501 - GFF928 0.30 -0.1 106,501 - GFF928 0.30 -0.8 106,545 - GFF928 0.41 -0.7 106,545 - GFF928 0.41 +0.1 106,545 - GFF928 0.41 -1.2 106,595 - GFF928 0.54 +1.0 106,700 - GFF928 0.81 +0.0 106,718 + GFF928 0.86 +1.0 106,719 - GFF928 0.86 -0.2 106,722 + GFF928 0.87 +0.1 106,723 - GFF928 0.87 +0.4 106,723 - GFF928 0.87 -1.1
Or see this region's nucleotide sequence