Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3500 and Pf6N2E2_3501 are separated by 3 nucleotides Pf6N2E2_3501 and Pf6N2E2_3502 overlap by 4 nucleotides Pf6N2E2_3502 and Pf6N2E2_3503 are separated by 48 nucleotides Pf6N2E2_3503 and Pf6N2E2_3504 are separated by 235 nucleotides
Pf6N2E2_3500 - DNA-damage-inducible protein F, at 4,080,058 to 4,081,407
_3500
Pf6N2E2_3501 - hypothetical protein, at 4,081,411 to 4,082,208
_3501
Pf6N2E2_3502 - VgrG protein, at 4,082,205 to 4,083,251
_3502
Pf6N2E2_3503 - hypothetical protein, at 4,083,300 to 4,083,650
_3503
Pf6N2E2_3504 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 4,083,886 to 4,085,799
_3504
Position (kb)
4082
4083
4084 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4081.302 kb on - strand at 4081.302 kb on - strand at 4081.405 kb on + strand at 4081.406 kb on - strand at 4081.552 kb on - strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.603 kb on - strand, within Pf6N2E2_3501 at 4081.603 kb on - strand, within Pf6N2E2_3501 at 4081.850 kb on + strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.862 kb on + strand, within Pf6N2E2_3501 at 4081.862 kb on + strand, within Pf6N2E2_3501 at 4081.863 kb on - strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4082.155 kb on + strand at 4082.156 kb on - strand at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.404 kb on - strand, within Pf6N2E2_3502 at 4082.404 kb on - strand, within Pf6N2E2_3502 at 4082.463 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.735 kb on - strand, within Pf6N2E2_3502 at 4082.936 kb on - strand, within Pf6N2E2_3502 at 4082.973 kb on - strand, within Pf6N2E2_3502 at 4083.042 kb on + strand, within Pf6N2E2_3502 at 4083.346 kb on + strand, within Pf6N2E2_3503 at 4083.346 kb on + strand, within Pf6N2E2_3503 at 4083.400 kb on + strand, within Pf6N2E2_3503 at 4083.400 kb on + strand, within Pf6N2E2_3503 at 4083.400 kb on + strand, within Pf6N2E2_3503 at 4083.401 kb on - strand, within Pf6N2E2_3503 at 4083.401 kb on - strand, within Pf6N2E2_3503 at 4083.401 kb on - strand, within Pf6N2E2_3503 at 4083.401 kb on - strand, within Pf6N2E2_3503 at 4083.472 kb on - strand, within Pf6N2E2_3503 at 4083.524 kb on + strand, within Pf6N2E2_3503 at 4083.569 kb on + strand, within Pf6N2E2_3503 at 4083.570 kb on - strand, within Pf6N2E2_3503 at 4083.570 kb on - strand, within Pf6N2E2_3503 at 4083.582 kb on + strand, within Pf6N2E2_3503 at 4083.582 kb on + strand, within Pf6N2E2_3503 at 4083.583 kb on - strand, within Pf6N2E2_3503 at 4083.583 kb on - strand, within Pf6N2E2_3503 at 4083.583 kb on - strand, within Pf6N2E2_3503 at 4083.583 kb on - strand, within Pf6N2E2_3503 at 4083.589 kb on + strand, within Pf6N2E2_3503 at 4083.590 kb on - strand, within Pf6N2E2_3503 at 4083.590 kb on - strand, within Pf6N2E2_3503 at 4083.602 kb on - strand, within Pf6N2E2_3503 at 4083.610 kb on + strand, within Pf6N2E2_3503 at 4083.611 kb on - strand, within Pf6N2E2_3503 at 4083.611 kb on - strand, within Pf6N2E2_3503 at 4083.627 kb on - strand at 4083.627 kb on - strand at 4083.640 kb on + strand at 4083.640 kb on + strand at 4083.640 kb on + strand at 4083.641 kb on - strand at 4083.648 kb on + strand at 4083.648 kb on + strand at 4083.663 kb on + strand at 4083.710 kb on + strand at 4083.710 kb on + strand at 4083.711 kb on - strand at 4083.740 kb on + strand at 4083.820 kb on + strand at 4084.025 kb on + strand at 4084.025 kb on + strand at 4084.073 kb on + strand at 4084.073 kb on + strand at 4084.097 kb on + strand, within Pf6N2E2_3504 at 4084.097 kb on + strand, within Pf6N2E2_3504 at 4084.097 kb on + strand, within Pf6N2E2_3504 at 4084.097 kb on + strand, within Pf6N2E2_3504 at 4084.187 kb on + strand, within Pf6N2E2_3504 at 4084.187 kb on + strand, within Pf6N2E2_3504 at 4084.187 kb on + strand, within Pf6N2E2_3504 at 4084.188 kb on - strand, within Pf6N2E2_3504 at 4084.250 kb on + strand, within Pf6N2E2_3504 at 4084.250 kb on + strand, within Pf6N2E2_3504 at 4084.251 kb on - strand, within Pf6N2E2_3504
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 4,081,302 - -1.5 4,081,302 - +0.4 4,081,405 + +0.4 4,081,406 - +0.2 4,081,552 - Pf6N2E2_3501 0.18 +0.7 4,081,598 + Pf6N2E2_3501 0.23 +0.7 4,081,598 + Pf6N2E2_3501 0.23 -0.1 4,081,598 + Pf6N2E2_3501 0.23 +0.9 4,081,599 - Pf6N2E2_3501 0.24 -0.1 4,081,599 - Pf6N2E2_3501 0.24 +0.4 4,081,599 - Pf6N2E2_3501 0.24 +0.1 4,081,602 + Pf6N2E2_3501 0.24 +0.2 4,081,602 + Pf6N2E2_3501 0.24 -0.4 4,081,602 + Pf6N2E2_3501 0.24 +0.1 4,081,602 + Pf6N2E2_3501 0.24 +0.5 4,081,602 + Pf6N2E2_3501 0.24 +0.5 4,081,602 + Pf6N2E2_3501 0.24 +1.9 4,081,603 - Pf6N2E2_3501 0.24 +0.3 4,081,603 - Pf6N2E2_3501 0.24 -1.8 4,081,850 + Pf6N2E2_3501 0.55 -0.0 4,081,851 - Pf6N2E2_3501 0.55 +0.7 4,081,851 - Pf6N2E2_3501 0.55 -0.3 4,081,851 - Pf6N2E2_3501 0.55 +0.8 4,081,862 + Pf6N2E2_3501 0.57 -0.6 4,081,862 + Pf6N2E2_3501 0.57 -0.8 4,081,863 - Pf6N2E2_3501 0.57 -0.4 4,081,871 + Pf6N2E2_3501 0.58 -0.4 4,081,871 + Pf6N2E2_3501 0.58 -0.4 4,081,871 + Pf6N2E2_3501 0.58 +0.9 4,081,872 - Pf6N2E2_3501 0.58 +0.2 4,081,872 - Pf6N2E2_3501 0.58 -0.9 4,081,872 - Pf6N2E2_3501 0.58 +0.9 4,081,872 - Pf6N2E2_3501 0.58 -0.5 4,081,872 - Pf6N2E2_3501 0.58 +0.4 4,081,874 + Pf6N2E2_3501 0.58 -0.2 4,081,874 + Pf6N2E2_3501 0.58 +1.0 4,081,874 + Pf6N2E2_3501 0.58 +1.7 4,081,875 - Pf6N2E2_3501 0.58 -1.3 4,081,875 - Pf6N2E2_3501 0.58 +0.4 4,081,875 - Pf6N2E2_3501 0.58 -0.2 4,081,875 - Pf6N2E2_3501 0.58 +0.3 4,081,875 - Pf6N2E2_3501 0.58 +0.6 4,081,875 - Pf6N2E2_3501 0.58 -0.8 4,081,875 - Pf6N2E2_3501 0.58 +1.3 4,081,875 - Pf6N2E2_3501 0.58 +1.2 4,082,155 + -1.4 4,082,156 - +0.5 4,082,403 + Pf6N2E2_3502 0.19 +0.3 4,082,403 + Pf6N2E2_3502 0.19 +0.5 4,082,403 + Pf6N2E2_3502 0.19 -0.5 4,082,404 - Pf6N2E2_3502 0.19 +1.0 4,082,404 - Pf6N2E2_3502 0.19 +0.7 4,082,463 - Pf6N2E2_3502 0.25 +0.7 4,082,573 - Pf6N2E2_3502 0.35 +0.5 4,082,573 - Pf6N2E2_3502 0.35 +0.3 4,082,573 - Pf6N2E2_3502 0.35 +1.5 4,082,735 - Pf6N2E2_3502 0.51 +0.2 4,082,936 - Pf6N2E2_3502 0.70 +1.0 4,082,973 - Pf6N2E2_3502 0.73 +1.0 4,083,042 + Pf6N2E2_3502 0.80 -2.1 4,083,346 + Pf6N2E2_3503 0.13 +0.0 4,083,346 + Pf6N2E2_3503 0.13 +1.3 4,083,400 + Pf6N2E2_3503 0.28 -0.9 4,083,400 + Pf6N2E2_3503 0.28 +0.2 4,083,400 + Pf6N2E2_3503 0.28 -0.2 4,083,401 - Pf6N2E2_3503 0.29 +1.1 4,083,401 - Pf6N2E2_3503 0.29 -0.4 4,083,401 - Pf6N2E2_3503 0.29 -0.3 4,083,401 - Pf6N2E2_3503 0.29 +0.6 4,083,472 - Pf6N2E2_3503 0.49 +0.3 4,083,524 + Pf6N2E2_3503 0.64 +0.5 4,083,569 + Pf6N2E2_3503 0.77 +0.2 4,083,570 - Pf6N2E2_3503 0.77 -0.0 4,083,570 - Pf6N2E2_3503 0.77 -0.1 4,083,582 + Pf6N2E2_3503 0.80 -0.7 4,083,582 + Pf6N2E2_3503 0.80 +0.5 4,083,583 - Pf6N2E2_3503 0.81 +0.8 4,083,583 - Pf6N2E2_3503 0.81 -0.2 4,083,583 - Pf6N2E2_3503 0.81 -0.3 4,083,583 - Pf6N2E2_3503 0.81 +0.9 4,083,589 + Pf6N2E2_3503 0.82 +0.3 4,083,590 - Pf6N2E2_3503 0.83 +0.9 4,083,590 - Pf6N2E2_3503 0.83 +1.0 4,083,602 - Pf6N2E2_3503 0.86 +0.6 4,083,610 + Pf6N2E2_3503 0.88 -1.2 4,083,611 - Pf6N2E2_3503 0.89 +0.6 4,083,611 - Pf6N2E2_3503 0.89 +0.7 4,083,627 - +1.3 4,083,627 - +1.3 4,083,640 + +0.4 4,083,640 + -1.5 4,083,640 + -0.7 4,083,641 - +0.7 4,083,648 + +0.3 4,083,648 + -0.2 4,083,663 + +0.2 4,083,710 + -0.6 4,083,710 + -0.0 4,083,711 - +0.4 4,083,740 + -0.0 4,083,820 + +0.1 4,084,025 + +0.1 4,084,025 + +1.0 4,084,073 + +0.8 4,084,073 + +0.5 4,084,097 + Pf6N2E2_3504 0.11 +0.6 4,084,097 + Pf6N2E2_3504 0.11 -0.6 4,084,097 + Pf6N2E2_3504 0.11 +0.3 4,084,097 + Pf6N2E2_3504 0.11 -1.0 4,084,187 + Pf6N2E2_3504 0.16 +0.9 4,084,187 + Pf6N2E2_3504 0.16 -0.7 4,084,187 + Pf6N2E2_3504 0.16 +0.1 4,084,188 - Pf6N2E2_3504 0.16 +0.1 4,084,250 + Pf6N2E2_3504 0.19 +1.0 4,084,250 + Pf6N2E2_3504 0.19 -0.1 4,084,251 - Pf6N2E2_3504 0.19 -1.4
Or see this region's nucleotide sequence