Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1310 and Pf6N2E2_1311 are separated by 91 nucleotides Pf6N2E2_1311 and Pf6N2E2_1312 are separated by 52 nucleotides Pf6N2E2_1312 and Pf6N2E2_1313 are separated by 12 nucleotides Pf6N2E2_1313 and Pf6N2E2_1314 are separated by 14 nucleotides
Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426
_1310
Pf6N2E2_1311 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 1,545,518 to 1,545,910
_1311
Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267
_1312
Pf6N2E2_1313 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 1,547,280 to 1,548,083
_1313
Pf6N2E2_1314 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,548,098 to 1,548,895
_1314
Position (kb)
1545
1546
1547
1548 Strain fitness (log2 ratio)
-2
-1
0
1 at 1544.965 kb on + strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1545.347 kb on - strand at 1545.450 kb on - strand at 1545.450 kb on - strand at 1545.480 kb on + strand at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1546.063 kb on - strand at 1546.311 kb on + strand, within Pf6N2E2_1312 at 1546.731 kb on + strand, within Pf6N2E2_1312 at 1546.732 kb on - strand, within Pf6N2E2_1312 at 1546.732 kb on - strand, within Pf6N2E2_1312 at 1546.732 kb on - strand, within Pf6N2E2_1312 at 1546.757 kb on + strand, within Pf6N2E2_1312 at 1547.140 kb on - strand at 1547.190 kb on + strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.191 kb on - strand at 1547.397 kb on + strand, within Pf6N2E2_1313 at 1547.398 kb on - strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.856 kb on + strand, within Pf6N2E2_1313 at 1547.953 kb on - strand, within Pf6N2E2_1313 at 1548.182 kb on + strand, within Pf6N2E2_1314
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,544,965 + Pf6N2E2_1310 0.55 +0.7 1,544,966 - Pf6N2E2_1310 0.55 +1.0 1,544,966 - Pf6N2E2_1310 0.55 +0.7 1,545,347 - +0.3 1,545,450 - -0.3 1,545,450 - +1.1 1,545,480 + -0.9 1,545,703 - Pf6N2E2_1311 0.47 -1.1 1,545,703 - Pf6N2E2_1311 0.47 +1.0 1,546,063 - +1.2 1,546,311 + Pf6N2E2_1312 0.27 +0.7 1,546,731 + Pf6N2E2_1312 0.59 -2.1 1,546,732 - Pf6N2E2_1312 0.59 -0.0 1,546,732 - Pf6N2E2_1312 0.59 -0.3 1,546,732 - Pf6N2E2_1312 0.59 -0.7 1,546,757 + Pf6N2E2_1312 0.61 -0.9 1,547,140 - +0.5 1,547,190 + -0.7 1,547,191 - -1.7 1,547,191 - -0.1 1,547,191 - +0.7 1,547,191 - +0.5 1,547,397 + Pf6N2E2_1313 0.15 +0.8 1,547,398 - Pf6N2E2_1313 0.15 +0.3 1,547,856 + Pf6N2E2_1313 0.72 -0.2 1,547,856 + Pf6N2E2_1313 0.72 -1.3 1,547,953 - Pf6N2E2_1313 0.84 +0.7 1,548,182 + Pf6N2E2_1314 0.11 -0.3
Or see this region's nucleotide sequence