Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2786 and Pf6N2E2_6113 are separated by 162 nucleotides Pf6N2E2_6113 and Pf6N2E2_2787 are separated by 835 nucleotides
Pf6N2E2_2786 - (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-), at 3,275,432 to 3,276,316
_2786
Pf6N2E2_6113 - tRNA-Gly-CCC, at 3,276,479 to 3,276,549
_6113
Pf6N2E2_2787 - hypothetical protein, at 3,277,385 to 3,277,498
_2787
Position (kb)
3276
3277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3275.481 kb on - strand at 3275.864 kb on + strand, within Pf6N2E2_2786 at 3275.955 kb on - strand, within Pf6N2E2_2786 at 3275.987 kb on + strand, within Pf6N2E2_2786 at 3275.988 kb on - strand, within Pf6N2E2_2786 at 3276.041 kb on + strand, within Pf6N2E2_2786 at 3276.041 kb on + strand, within Pf6N2E2_2786 at 3276.042 kb on - strand, within Pf6N2E2_2786 at 3276.042 kb on - strand, within Pf6N2E2_2786 at 3276.042 kb on - strand, within Pf6N2E2_2786 at 3276.104 kb on + strand, within Pf6N2E2_2786 at 3276.104 kb on + strand, within Pf6N2E2_2786 at 3276.104 kb on + strand, within Pf6N2E2_2786 at 3276.104 kb on + strand, within Pf6N2E2_2786 at 3276.105 kb on - strand, within Pf6N2E2_2786 at 3276.149 kb on + strand, within Pf6N2E2_2786 at 3276.150 kb on - strand, within Pf6N2E2_2786 at 3276.150 kb on - strand, within Pf6N2E2_2786 at 3276.279 kb on - strand at 3276.279 kb on - strand at 3276.279 kb on - strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.284 kb on + strand at 3276.285 kb on - strand at 3276.285 kb on - strand at 3276.285 kb on - strand at 3276.285 kb on - strand at 3276.285 kb on - strand at 3276.285 kb on - strand at 3276.296 kb on + strand at 3276.346 kb on + strand at 3276.403 kb on - strand at 3276.421 kb on + strand at 3276.421 kb on + strand at 3276.421 kb on + strand at 3276.422 kb on - strand at 3276.425 kb on + strand at 3276.426 kb on - strand at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.489 kb on + strand, within Pf6N2E2_6113 at 3276.490 kb on - strand, within Pf6N2E2_6113 at 3276.490 kb on - strand, within Pf6N2E2_6113 at 3276.490 kb on - strand, within Pf6N2E2_6113 at 3276.492 kb on - strand, within Pf6N2E2_6113 at 3276.492 kb on - strand, within Pf6N2E2_6113 at 3276.501 kb on - strand, within Pf6N2E2_6113 at 3276.673 kb on - strand at 3276.673 kb on - strand at 3276.673 kb on - strand at 3276.673 kb on - strand at 3276.676 kb on + strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.677 kb on - strand at 3276.711 kb on + strand at 3276.711 kb on + strand at 3276.711 kb on + strand at 3276.712 kb on - strand at 3276.712 kb on - strand at 3276.712 kb on - strand at 3276.719 kb on + strand at 3276.719 kb on + strand at 3276.720 kb on - strand at 3276.723 kb on + strand at 3276.723 kb on + strand at 3276.723 kb on + strand at 3276.724 kb on - strand at 3276.724 kb on - strand at 3276.744 kb on - strand at 3276.914 kb on + strand at 3276.915 kb on - strand at 3276.915 kb on - strand at 3276.915 kb on - strand at 3276.915 kb on - strand at 3276.946 kb on + strand at 3276.947 kb on - strand at 3276.947 kb on - strand at 3276.990 kb on + strand at 3276.991 kb on - strand at 3277.030 kb on + strand at 3277.031 kb on - strand at 3277.031 kb on - strand at 3277.157 kb on + strand at 3277.158 kb on - strand at 3277.164 kb on + strand at 3277.164 kb on + strand at 3277.171 kb on - strand at 3277.171 kb on - strand at 3277.220 kb on + strand at 3277.221 kb on - strand at 3277.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,275,481 - -1.3 3,275,864 + Pf6N2E2_2786 0.49 -1.0 3,275,955 - Pf6N2E2_2786 0.59 +0.4 3,275,987 + Pf6N2E2_2786 0.63 +1.1 3,275,988 - Pf6N2E2_2786 0.63 +0.7 3,276,041 + Pf6N2E2_2786 0.69 +0.8 3,276,041 + Pf6N2E2_2786 0.69 +1.0 3,276,042 - Pf6N2E2_2786 0.69 -0.8 3,276,042 - Pf6N2E2_2786 0.69 -0.1 3,276,042 - Pf6N2E2_2786 0.69 +1.0 3,276,104 + Pf6N2E2_2786 0.76 +0.8 3,276,104 + Pf6N2E2_2786 0.76 -3.6 3,276,104 + Pf6N2E2_2786 0.76 +0.6 3,276,104 + Pf6N2E2_2786 0.76 -0.3 3,276,105 - Pf6N2E2_2786 0.76 +0.6 3,276,149 + Pf6N2E2_2786 0.81 -0.2 3,276,150 - Pf6N2E2_2786 0.81 +0.6 3,276,150 - Pf6N2E2_2786 0.81 -1.4 3,276,279 - +0.4 3,276,279 - +0.5 3,276,279 - +0.4 3,276,284 + +0.7 3,276,284 + +0.1 3,276,284 + +0.0 3,276,284 + -0.5 3,276,284 + +0.1 3,276,284 + -0.8 3,276,284 + +0.6 3,276,285 - -0.0 3,276,285 - -0.1 3,276,285 - +0.7 3,276,285 - +1.6 3,276,285 - -1.1 3,276,285 - +1.1 3,276,296 + -0.2 3,276,346 + -0.6 3,276,403 - +0.3 3,276,421 + +1.3 3,276,421 + +1.1 3,276,421 + +1.2 3,276,422 - +0.1 3,276,425 + +1.7 3,276,426 - -0.0 3,276,489 + Pf6N2E2_6113 0.14 +0.7 3,276,489 + Pf6N2E2_6113 0.14 +0.4 3,276,489 + Pf6N2E2_6113 0.14 +0.9 3,276,489 + Pf6N2E2_6113 0.14 +1.3 3,276,489 + Pf6N2E2_6113 0.14 +0.2 3,276,489 + Pf6N2E2_6113 0.14 -1.3 3,276,490 - Pf6N2E2_6113 0.15 +1.1 3,276,490 - Pf6N2E2_6113 0.15 +2.0 3,276,490 - Pf6N2E2_6113 0.15 +0.8 3,276,492 - Pf6N2E2_6113 0.18 +0.6 3,276,492 - Pf6N2E2_6113 0.18 -0.0 3,276,501 - Pf6N2E2_6113 0.31 +0.8 3,276,673 - +0.4 3,276,673 - -0.9 3,276,673 - -0.5 3,276,673 - +0.2 3,276,676 + +1.5 3,276,677 - -0.1 3,276,677 - +1.0 3,276,677 - +0.5 3,276,677 - +1.2 3,276,677 - +0.5 3,276,677 - +0.1 3,276,677 - -0.9 3,276,677 - +0.4 3,276,677 - -0.9 3,276,677 - -0.9 3,276,677 - -0.7 3,276,677 - +0.3 3,276,677 - +0.5 3,276,677 - -1.6 3,276,711 + -0.4 3,276,711 + -1.0 3,276,711 + +1.0 3,276,712 - +0.2 3,276,712 - +0.2 3,276,712 - -0.6 3,276,719 + +0.3 3,276,719 + +1.8 3,276,720 - -0.3 3,276,723 + +0.6 3,276,723 + +0.1 3,276,723 + +0.8 3,276,724 - +1.3 3,276,724 - -0.7 3,276,744 - -1.3 3,276,914 + +0.0 3,276,915 - +0.6 3,276,915 - -1.3 3,276,915 - +0.7 3,276,915 - +0.4 3,276,946 + +0.7 3,276,947 - -0.1 3,276,947 - +0.3 3,276,990 + -0.3 3,276,991 - +0.3 3,277,030 + -0.2 3,277,031 - +1.4 3,277,031 - +0.3 3,277,157 + -0.3 3,277,158 - -0.4 3,277,164 + +0.5 3,277,164 + +0.5 3,277,171 - -0.4 3,277,171 - +0.2 3,277,220 + +0.4 3,277,221 - -0.8 3,277,508 + +0.2
Or see this region's nucleotide sequence