Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1148 and Pf6N2E2_1149 are separated by 3 nucleotides Pf6N2E2_1149 and Pf6N2E2_1150 are separated by 134 nucleotides
Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582
_1148
Pf6N2E2_1149 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 1,346,586 to 1,347,692
_1149
Pf6N2E2_1150 - CBS-domain-containing membrane protein, at 1,347,827 to 1,348,534
_1150
Position (kb)
1346
1347
1348 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1345.789 kb on + strand, within Pf6N2E2_1148 at 1346.012 kb on + strand, within Pf6N2E2_1148 at 1346.392 kb on + strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.396 kb on + strand, within Pf6N2E2_1148 at 1346.418 kb on + strand, within Pf6N2E2_1148 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.862 kb on + strand, within Pf6N2E2_1149 at 1346.863 kb on - strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.202 kb on + strand, within Pf6N2E2_1149 at 1347.436 kb on + strand, within Pf6N2E2_1149 at 1347.437 kb on - strand, within Pf6N2E2_1149 at 1347.738 kb on + strand at 1347.739 kb on - strand at 1347.807 kb on + strand at 1347.807 kb on + strand at 1347.808 kb on - strand at 1347.811 kb on + strand at 1347.811 kb on + strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.141 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.669 kb on + strand at 1348.670 kb on - strand at 1348.670 kb on - strand at 1348.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,345,789 + Pf6N2E2_1148 0.31 +0.5 1,346,012 + Pf6N2E2_1148 0.50 +0.2 1,346,392 + Pf6N2E2_1148 0.83 -0.6 1,346,393 - Pf6N2E2_1148 0.84 +0.0 1,346,393 - Pf6N2E2_1148 0.84 -1.1 1,346,393 - Pf6N2E2_1148 0.84 +0.9 1,346,396 + Pf6N2E2_1148 0.84 +1.5 1,346,418 + Pf6N2E2_1148 0.86 +0.2 1,346,703 + Pf6N2E2_1149 0.11 -0.7 1,346,703 + Pf6N2E2_1149 0.11 -0.5 1,346,704 - Pf6N2E2_1149 0.11 +0.9 1,346,704 - Pf6N2E2_1149 0.11 +0.4 1,346,862 + Pf6N2E2_1149 0.25 -0.8 1,346,863 - Pf6N2E2_1149 0.25 +0.8 1,347,038 + Pf6N2E2_1149 0.41 -0.1 1,347,038 + Pf6N2E2_1149 0.41 -0.4 1,347,121 + Pf6N2E2_1149 0.48 +0.3 1,347,121 + Pf6N2E2_1149 0.48 +0.5 1,347,122 - Pf6N2E2_1149 0.48 +0.9 1,347,122 - Pf6N2E2_1149 0.48 +0.2 1,347,122 - Pf6N2E2_1149 0.48 +0.7 1,347,122 - Pf6N2E2_1149 0.48 -1.8 1,347,122 - Pf6N2E2_1149 0.48 +0.2 1,347,166 + Pf6N2E2_1149 0.52 +0.3 1,347,166 + Pf6N2E2_1149 0.52 +0.6 1,347,167 - Pf6N2E2_1149 0.52 -0.1 1,347,167 - Pf6N2E2_1149 0.52 +1.6 1,347,202 + Pf6N2E2_1149 0.56 -3.0 1,347,436 + Pf6N2E2_1149 0.77 +0.0 1,347,437 - Pf6N2E2_1149 0.77 -0.1 1,347,738 + +1.7 1,347,739 - -0.0 1,347,807 + -0.6 1,347,807 + -0.9 1,347,808 - -0.9 1,347,811 + -1.0 1,347,811 + -0.4 1,347,812 - -0.5 1,347,812 - +0.3 1,347,812 - +1.5 1,347,812 - +0.0 1,347,812 - +0.7 1,347,812 - -0.5 1,348,063 + Pf6N2E2_1150 0.33 -0.6 1,348,063 + Pf6N2E2_1150 0.33 +0.3 1,348,065 + Pf6N2E2_1150 0.34 +0.1 1,348,065 + Pf6N2E2_1150 0.34 +0.2 1,348,065 + Pf6N2E2_1150 0.34 +0.4 1,348,141 + Pf6N2E2_1150 0.44 -0.3 1,348,268 + Pf6N2E2_1150 0.62 +0.1 1,348,268 + Pf6N2E2_1150 0.62 +0.2 1,348,269 - Pf6N2E2_1150 0.62 -0.1 1,348,269 - Pf6N2E2_1150 0.62 +0.2 1,348,269 - Pf6N2E2_1150 0.62 +0.8 1,348,669 + -0.0 1,348,670 - -0.8 1,348,670 - -0.9 1,348,670 - -0.4
Or see this region's nucleotide sequence