Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4494 and Pf6N2E2_4495 are separated by 24 nucleotides Pf6N2E2_4495 and Pf6N2E2_4496 are separated by 14 nucleotides
Pf6N2E2_4494 - Adenylate cyclase (EC 4.6.1.1), at 5,226,306 to 5,227,673
_4494
Pf6N2E2_4495 - hypothetical protein, at 5,227,698 to 5,227,817
_4495
Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989
_4496
Position (kb)
5227
5228 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.049 kb on - strand, within Pf6N2E2_4494 at 5227.064 kb on - strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.417 kb on + strand, within Pf6N2E2_4494 at 5227.418 kb on - strand, within Pf6N2E2_4494 at 5227.549 kb on + strand at 5227.549 kb on + strand at 5227.550 kb on - strand at 5227.550 kb on - strand at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.739 kb on + strand, within Pf6N2E2_4495 at 5227.758 kb on - strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand, within Pf6N2E2_4495 at 5227.796 kb on + strand at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.797 kb on - strand, within Pf6N2E2_4495 at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.815 kb on + strand at 5227.816 kb on - strand at 5227.816 kb on - strand at 5228.046 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.686 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496 at 5228.702 kb on + strand, within Pf6N2E2_4496
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 5,227,049 - Pf6N2E2_4494 0.54 -1.0 5,227,049 - Pf6N2E2_4494 0.54 +0.6 5,227,064 - Pf6N2E2_4494 0.55 +0.6 5,227,417 + Pf6N2E2_4494 0.81 +0.2 5,227,417 + Pf6N2E2_4494 0.81 +0.1 5,227,418 - Pf6N2E2_4494 0.81 -2.3 5,227,549 + +1.2 5,227,549 + +1.0 5,227,550 - -0.2 5,227,550 - -0.4 5,227,739 + Pf6N2E2_4495 0.34 -0.2 5,227,739 + Pf6N2E2_4495 0.34 -0.3 5,227,739 + Pf6N2E2_4495 0.34 +1.1 5,227,739 + Pf6N2E2_4495 0.34 +2.0 5,227,739 + Pf6N2E2_4495 0.34 +1.1 5,227,758 - Pf6N2E2_4495 0.50 +0.7 5,227,796 + Pf6N2E2_4495 0.82 +0.6 5,227,796 + Pf6N2E2_4495 0.82 +0.9 5,227,796 + Pf6N2E2_4495 0.82 -0.4 5,227,796 + Pf6N2E2_4495 0.82 -0.5 5,227,796 + Pf6N2E2_4495 0.82 +1.2 5,227,796 + Pf6N2E2_4495 0.82 +1.4 5,227,796 + Pf6N2E2_4495 0.82 -0.0 5,227,796 + Pf6N2E2_4495 0.82 -0.0 5,227,796 + Pf6N2E2_4495 0.82 +1.5 5,227,796 + +1.2 5,227,797 - Pf6N2E2_4495 0.82 +0.8 5,227,797 - Pf6N2E2_4495 0.82 +0.1 5,227,797 - Pf6N2E2_4495 0.82 -0.6 5,227,797 - Pf6N2E2_4495 0.82 -0.1 5,227,797 - Pf6N2E2_4495 0.82 +0.1 5,227,797 - Pf6N2E2_4495 0.82 +0.5 5,227,797 - Pf6N2E2_4495 0.82 +0.6 5,227,815 + +0.9 5,227,815 + +0.7 5,227,815 + +0.1 5,227,816 - -1.6 5,227,816 - -0.5 5,228,046 + Pf6N2E2_4496 0.18 +0.2 5,228,686 + Pf6N2E2_4496 0.74 +0.2 5,228,686 + Pf6N2E2_4496 0.74 +1.1 5,228,702 + Pf6N2E2_4496 0.75 +2.0 5,228,702 + Pf6N2E2_4496 0.75 +0.4
Or see this region's nucleotide sequence