Strain Fitness in Escherichia coli BL21 around ECD_00141

Experiment: CM1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00140 and ECD_00141 are separated by 369 nucleotidesECD_00141 and ECD_00142 overlap by 4 nucleotides ECD_00140: ECD_00140 - putative fimbrial-like adhesin protein, at 159,151 to 159,735 _00140 ECD_00141: ECD_00141 - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase, at 160,105 to 160,584 _00141 ECD_00142: ECD_00142 - poly(A) polymerase, at 160,581 to 161,945 _00142 Position (kb) 160 161Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 159.339 kb on + strand, within ECD_00140at 160.572 kb on - strandat 160.572 kb on - strandat 160.778 kb on - strand, within ECD_00142at 160.842 kb on - strand, within ECD_00142at 161.174 kb on - strand, within ECD_00142at 161.190 kb on - strand, within ECD_00142at 161.192 kb on - strand, within ECD_00142at 161.355 kb on - strand, within ECD_00142at 161.474 kb on - strand, within ECD_00142at 161.474 kb on - strand, within ECD_00142

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Per-strain Table

Position Strand Gene LocusTag Fraction CM1
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159,339 + ECD_00140 0.32 +0.9
160,572 - +0.7
160,572 - +4.7
160,778 - ECD_00142 0.14 +0.3
160,842 - ECD_00142 0.19 -0.5
161,174 - ECD_00142 0.43 -2.4
161,190 - ECD_00142 0.45 -0.9
161,192 - ECD_00142 0.45 -2.1
161,355 - ECD_00142 0.57 -1.2
161,474 - ECD_00142 0.65 -1.8
161,474 - ECD_00142 0.65 -1.8

Or see this region's nucleotide sequence