Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04775

Experiment: McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04770 and MMJJ_RS04775 are separated by 180 nucleotidesMMJJ_RS04775 and MMJJ_RS04780 are separated by 3 nucleotidesMMJJ_RS04780 and MMJJ_RS04785 are separated by 12 nucleotidesMMJJ_RS04785 and MMJJ_RS04790 overlap by 20 nucleotides MMJJ_RS04770: MMJJ_RS04770 - formate-dependent phosphoribosylglycinamide formyltransferase, at 895,374 to 896,540 _RS04770 MMJJ_RS04775: MMJJ_RS04775 - DNA-binding protein, at 896,721 to 897,632 _RS04775 MMJJ_RS04780: MMJJ_RS04780 - nucleic acid-binding protein, at 897,636 to 897,953 _RS04780 MMJJ_RS04785: MMJJ_RS04785 - OB-fold nucleic acid binding domain-containing protein, at 897,966 to 898,352 _RS04785 MMJJ_RS04790: MMJJ_RS04790 - biotin--[acetyl-CoA-carboxylase] ligase, at 898,333 to 899,058 _RS04790 Position (kb) 896 897 898Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 896.085 kb on - strand, within MMJJ_RS04770at 896.086 kb on + strand, within MMJJ_RS04770at 896.090 kb on - strandat 896.280 kb on + strand, within MMJJ_RS04770at 896.310 kb on - strand, within MMJJ_RS04770at 896.558 kb on - strandat 896.598 kb on - strandat 896.610 kb on - strandat 896.610 kb on - strandat 896.610 kb on - strandat 896.610 kb on - strandat 896.611 kb on + strandat 896.613 kb on - strandat 896.613 kb on - strandat 896.613 kb on - strandat 896.614 kb on + strandat 896.614 kb on + strandat 896.615 kb on - strandat 896.615 kb on - strandat 896.615 kb on - strandat 896.616 kb on + strandat 896.616 kb on + strandat 896.616 kb on + strandat 896.638 kb on + strandat 896.686 kb on - strandat 896.686 kb on - strandat 896.706 kb on - strandat 896.750 kb on - strandat 896.751 kb on + strandat 896.751 kb on + strandat 896.751 kb on + strandat 896.752 kb on - strandat 896.752 kb on - strandat 896.752 kb on - strandat 896.781 kb on + strandat 896.810 kb on - strandat 896.812 kb on - strandat 896.812 kb on - strandat 896.813 kb on + strand, within MMJJ_RS04775at 896.813 kb on + strand, within MMJJ_RS04775at 896.813 kb on + strand, within MMJJ_RS04775at 896.813 kb on + strand, within MMJJ_RS04775at 896.813 kb on + strand, within MMJJ_RS04775at 896.813 kb on + strand, within MMJJ_RS04775at 896.816 kb on - strand, within MMJJ_RS04775at 896.817 kb on + strand, within MMJJ_RS04775at 896.817 kb on + strand, within MMJJ_RS04775at 896.870 kb on - strand, within MMJJ_RS04775at 896.910 kb on + strand, within MMJJ_RS04775at 896.921 kb on + strand, within MMJJ_RS04775at 896.960 kb on + strand, within MMJJ_RS04775at 897.019 kb on - strand, within MMJJ_RS04775at 897.067 kb on - strand, within MMJJ_RS04775at 897.068 kb on + strand, within MMJJ_RS04775at 897.187 kb on - strand, within MMJJ_RS04775at 897.188 kb on + strand, within MMJJ_RS04775at 897.364 kb on - strand, within MMJJ_RS04775at 897.413 kb on + strand, within MMJJ_RS04775at 897.413 kb on + strand, within MMJJ_RS04775at 897.417 kb on + strand, within MMJJ_RS04775at 897.423 kb on + strand, within MMJJ_RS04775at 897.423 kb on + strand, within MMJJ_RS04775at 897.518 kb on + strand, within MMJJ_RS04775at 897.520 kb on - strand, within MMJJ_RS04775at 897.538 kb on - strand, within MMJJ_RS04775at 897.539 kb on + strand, within MMJJ_RS04775at 897.540 kb on - strand, within MMJJ_RS04775at 897.541 kb on + strandat 897.541 kb on + strandat 897.554 kb on + strandat 897.570 kb on + strandat 897.596 kb on - strandat 897.597 kb on + strandat 897.598 kb on - strandat 897.639 kb on - strandat 897.640 kb on + strandat 897.802 kb on - strand, within MMJJ_RS04780at 897.803 kb on + strand, within MMJJ_RS04780at 897.806 kb on - strand, within MMJJ_RS04780at 897.807 kb on + strand, within MMJJ_RS04780at 897.863 kb on - strand, within MMJJ_RS04780at 897.864 kb on + strand, within MMJJ_RS04780at 897.883 kb on - strand, within MMJJ_RS04780at 897.884 kb on + strand, within MMJJ_RS04780at 897.884 kb on + strand, within MMJJ_RS04780at 898.003 kb on - strandat 898.004 kb on + strandat 898.050 kb on - strand, within MMJJ_RS04785at 898.050 kb on - strand, within MMJJ_RS04785at 898.050 kb on - strand, within MMJJ_RS04785at 898.052 kb on - strand, within MMJJ_RS04785at 898.052 kb on - strand, within MMJJ_RS04785at 898.053 kb on + strand, within MMJJ_RS04785at 898.053 kb on + strand, within MMJJ_RS04785at 898.053 kb on + strand, within MMJJ_RS04785at 898.062 kb on + strand, within MMJJ_RS04785at 898.093 kb on - strand, within MMJJ_RS04785at 898.103 kb on + strand, within MMJJ_RS04785at 898.145 kb on - strand, within MMJJ_RS04785at 898.145 kb on - strand, within MMJJ_RS04785at 898.268 kb on + strand, within MMJJ_RS04785at 898.277 kb on - strand, within MMJJ_RS04785at 898.277 kb on - strand, within MMJJ_RS04785at 898.277 kb on - strand, within MMJJ_RS04785at 898.278 kb on + strand, within MMJJ_RS04785at 898.281 kb on - strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.282 kb on + strand, within MMJJ_RS04785at 898.285 kb on - strand, within MMJJ_RS04785at 898.286 kb on + strand, within MMJJ_RS04785at 898.286 kb on + strand, within MMJJ_RS04785at 898.330 kb on - strandat 898.336 kb on - strandat 898.336 kb on - strandat 898.337 kb on + strandat 898.337 kb on + strandat 898.337 kb on + strandat 898.351 kb on + strandat 898.351 kb on + strandat 898.411 kb on - strand, within MMJJ_RS04790at 898.412 kb on + strand, within MMJJ_RS04790at 898.412 kb on + strand, within MMJJ_RS04790at 898.485 kb on - strand, within MMJJ_RS04790at 898.602 kb on + strand, within MMJJ_RS04790

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 2,2-dibromo-2-cyanoacetamide 0.00833333333333333 mM
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896,085 - MMJJ_RS04770 0.61 +0.5
896,086 + MMJJ_RS04770 0.61 -1.2
896,090 - +0.8
896,280 + MMJJ_RS04770 0.78 -1.4
896,310 - MMJJ_RS04770 0.80 +0.5
896,558 - -1.0
896,598 - +2.7
896,610 - -0.4
896,610 - +1.3
896,610 - +3.0
896,610 - -0.7
896,611 + -2.7
896,613 - -2.8
896,613 - -0.7
896,613 - -0.5
896,614 + -3.0
896,614 + -1.8
896,615 - -1.0
896,615 - -0.3
896,615 - -3.7
896,616 + +0.9
896,616 + -0.5
896,616 + -3.1
896,638 + -0.5
896,686 - -0.9
896,686 - -2.8
896,706 - -2.5
896,750 - -0.7
896,751 + -2.3
896,751 + -1.1
896,751 + +0.9
896,752 - +2.5
896,752 - -3.7
896,752 - +0.2
896,781 + +0.5
896,810 - -4.2
896,812 - -3.1
896,812 - +0.8
896,813 + MMJJ_RS04775 0.10 +3.6
896,813 + MMJJ_RS04775 0.10 +0.5
896,813 + MMJJ_RS04775 0.10 -4.4
896,813 + MMJJ_RS04775 0.10 +2.4
896,813 + MMJJ_RS04775 0.10 +1.8
896,813 + MMJJ_RS04775 0.10 -0.6
896,816 - MMJJ_RS04775 0.10 +2.8
896,817 + MMJJ_RS04775 0.11 -1.0
896,817 + MMJJ_RS04775 0.11 -1.7
896,870 - MMJJ_RS04775 0.16 +0.5
896,910 + MMJJ_RS04775 0.21 -3.4
896,921 + MMJJ_RS04775 0.22 +0.3
896,960 + MMJJ_RS04775 0.26 +3.7
897,019 - MMJJ_RS04775 0.33 -0.2
897,067 - MMJJ_RS04775 0.38 -0.0
897,068 + MMJJ_RS04775 0.38 +2.3
897,187 - MMJJ_RS04775 0.51 +0.1
897,188 + MMJJ_RS04775 0.51 +0.5
897,364 - MMJJ_RS04775 0.71 -0.3
897,413 + MMJJ_RS04775 0.76 -2.1
897,413 + MMJJ_RS04775 0.76 +0.3
897,417 + MMJJ_RS04775 0.76 -0.3
897,423 + MMJJ_RS04775 0.77 +2.6
897,423 + MMJJ_RS04775 0.77 +3.2
897,518 + MMJJ_RS04775 0.87 -1.2
897,520 - MMJJ_RS04775 0.88 +0.7
897,538 - MMJJ_RS04775 0.90 -0.7
897,539 + MMJJ_RS04775 0.90 +2.1
897,540 - MMJJ_RS04775 0.90 +4.0
897,541 + +0.0
897,541 + +3.2
897,554 + -2.1
897,570 + +3.0
897,596 - -1.1
897,597 + +1.3
897,598 - +1.1
897,639 - -0.2
897,640 + -2.0
897,802 - MMJJ_RS04780 0.52 +0.5
897,803 + MMJJ_RS04780 0.53 +0.4
897,806 - MMJJ_RS04780 0.53 +2.2
897,807 + MMJJ_RS04780 0.54 +1.1
897,863 - MMJJ_RS04780 0.71 -3.0
897,864 + MMJJ_RS04780 0.72 -0.1
897,883 - MMJJ_RS04780 0.78 -2.7
897,884 + MMJJ_RS04780 0.78 +0.3
897,884 + MMJJ_RS04780 0.78 -1.5
898,003 - -1.8
898,004 + +1.5
898,050 - MMJJ_RS04785 0.22 -2.6
898,050 - MMJJ_RS04785 0.22 +0.8
898,050 - MMJJ_RS04785 0.22 -0.7
898,052 - MMJJ_RS04785 0.22 +3.6
898,052 - MMJJ_RS04785 0.22 -0.2
898,053 + MMJJ_RS04785 0.22 -0.9
898,053 + MMJJ_RS04785 0.22 -3.4
898,053 + MMJJ_RS04785 0.22 -1.5
898,062 + MMJJ_RS04785 0.25 +1.6
898,093 - MMJJ_RS04785 0.33 +1.7
898,103 + MMJJ_RS04785 0.35 +1.3
898,145 - MMJJ_RS04785 0.46 +0.3
898,145 - MMJJ_RS04785 0.46 -1.7
898,268 + MMJJ_RS04785 0.78 -2.8
898,277 - MMJJ_RS04785 0.80 -3.5
898,277 - MMJJ_RS04785 0.80 -3.5
898,277 - MMJJ_RS04785 0.80 -1.6
898,278 + MMJJ_RS04785 0.81 -2.5
898,281 - MMJJ_RS04785 0.81 +1.0
898,282 + MMJJ_RS04785 0.82 -1.7
898,282 + MMJJ_RS04785 0.82 -0.5
898,282 + MMJJ_RS04785 0.82 -1.9
898,282 + MMJJ_RS04785 0.82 +3.3
898,282 + MMJJ_RS04785 0.82 +1.1
898,282 + MMJJ_RS04785 0.82 -0.1
898,282 + MMJJ_RS04785 0.82 -1.6
898,285 - MMJJ_RS04785 0.82 +0.1
898,286 + MMJJ_RS04785 0.83 -2.9
898,286 + MMJJ_RS04785 0.83 +3.4
898,330 - -2.9
898,336 - -2.9
898,336 - +1.3
898,337 + +1.5
898,337 + +0.2
898,337 + +2.3
898,351 + -1.1
898,351 + +0.0
898,411 - MMJJ_RS04790 0.11 -1.5
898,412 + MMJJ_RS04790 0.11 -1.5
898,412 + MMJJ_RS04790 0.11 +0.5
898,485 - MMJJ_RS04790 0.21 -1.3
898,602 + MMJJ_RS04790 0.37 +0.5

Or see this region's nucleotide sequence