Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02474 and MPMX20_02475 overlap by 4 nucleotides MPMX20_02475 and MPMX20_02476 overlap by 14 nucleotides MPMX20_02476 and MPMX20_02477 overlap by 1 nucleotides
MPMX20_02474: MPMX20_02474 - Peptide transport periplasmic protein SapA, at 2,613,982 to 2,615,622
_02474
MPMX20_02475: MPMX20_02475 - Putrescine export system permease protein SapB, at 2,615,619 to 2,616,584
_02475
MPMX20_02476: MPMX20_02476 - Putrescine export system permease protein SapC, at 2,616,571 to 2,617,461
_02476
MPMX20_02477: MPMX20_02477 - Peptide transport system ATP-binding protein SapD, at 2,617,461 to 2,618,453
_02477
Position (kb)
2615
2616
2617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2614.858 kb on + strand, within MPMX20_02474 at 2614.863 kb on + strand, within MPMX20_02474 at 2614.866 kb on - strand, within MPMX20_02474 at 2614.905 kb on + strand, within MPMX20_02474 at 2614.913 kb on - strand, within MPMX20_02474 at 2614.915 kb on + strand, within MPMX20_02474 at 2614.979 kb on - strand, within MPMX20_02474 at 2615.023 kb on + strand, within MPMX20_02474 at 2615.023 kb on + strand, within MPMX20_02474 at 2615.025 kb on - strand, within MPMX20_02474 at 2615.031 kb on - strand, within MPMX20_02474 at 2615.031 kb on - strand, within MPMX20_02474 at 2615.079 kb on + strand, within MPMX20_02474 at 2615.102 kb on + strand, within MPMX20_02474 at 2615.123 kb on - strand, within MPMX20_02474 at 2615.232 kb on - strand, within MPMX20_02474 at 2615.329 kb on + strand, within MPMX20_02474 at 2615.337 kb on - strand, within MPMX20_02474 at 2615.425 kb on - strand, within MPMX20_02474 at 2615.499 kb on + strand at 2615.499 kb on + strand at 2615.576 kb on + strand at 2615.685 kb on - strand at 2615.709 kb on - strand at 2615.823 kb on - strand, within MPMX20_02475 at 2615.823 kb on - strand, within MPMX20_02475 at 2615.823 kb on - strand, within MPMX20_02475 at 2615.825 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.848 kb on + strand, within MPMX20_02475 at 2615.856 kb on - strand, within MPMX20_02475 at 2615.960 kb on + strand, within MPMX20_02475 at 2615.964 kb on - strand, within MPMX20_02475 at 2616.022 kb on + strand, within MPMX20_02475 at 2616.027 kb on + strand, within MPMX20_02475 at 2616.126 kb on + strand, within MPMX20_02475 at 2616.172 kb on + strand, within MPMX20_02475 at 2616.235 kb on + strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.243 kb on - strand, within MPMX20_02475 at 2616.328 kb on - strand, within MPMX20_02475 at 2616.412 kb on + strand, within MPMX20_02475 at 2616.425 kb on - strand, within MPMX20_02475 at 2616.425 kb on - strand, within MPMX20_02475 at 2616.542 kb on + strand at 2616.585 kb on + strand at 2616.721 kb on - strand, within MPMX20_02476 at 2616.789 kb on + strand, within MPMX20_02476 at 2616.931 kb on - strand, within MPMX20_02476 at 2616.981 kb on - strand, within MPMX20_02476 at 2617.099 kb on + strand, within MPMX20_02476 at 2617.150 kb on + strand, within MPMX20_02476 at 2617.158 kb on - strand, within MPMX20_02476 at 2617.296 kb on + strand, within MPMX20_02476 at 2617.349 kb on + strand, within MPMX20_02476 at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.426 kb on + strand at 2617.434 kb on - strand at 2617.434 kb on - strand at 2617.540 kb on + strand at 2617.546 kb on + strand at 2617.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 2,614,858 + MPMX20_02474 0.53 -0.4 2,614,863 + MPMX20_02474 0.54 +0.6 2,614,866 - MPMX20_02474 0.54 -0.4 2,614,905 + MPMX20_02474 0.56 -1.5 2,614,913 - MPMX20_02474 0.57 +0.5 2,614,915 + MPMX20_02474 0.57 +0.3 2,614,979 - MPMX20_02474 0.61 -1.1 2,615,023 + MPMX20_02474 0.63 +1.4 2,615,023 + MPMX20_02474 0.63 -0.2 2,615,025 - MPMX20_02474 0.64 -0.6 2,615,031 - MPMX20_02474 0.64 +0.1 2,615,031 - MPMX20_02474 0.64 -0.2 2,615,079 + MPMX20_02474 0.67 +0.1 2,615,102 + MPMX20_02474 0.68 -0.6 2,615,123 - MPMX20_02474 0.70 +1.6 2,615,232 - MPMX20_02474 0.76 -1.9 2,615,329 + MPMX20_02474 0.82 -0.1 2,615,337 - MPMX20_02474 0.83 +1.2 2,615,425 - MPMX20_02474 0.88 -1.8 2,615,499 + -2.9 2,615,499 + +0.9 2,615,576 + +0.6 2,615,685 - -0.4 2,615,709 - -0.1 2,615,823 - MPMX20_02475 0.21 -1.4 2,615,823 - MPMX20_02475 0.21 -0.2 2,615,823 - MPMX20_02475 0.21 -0.1 2,615,825 + MPMX20_02475 0.21 -1.1 2,615,848 + MPMX20_02475 0.24 +0.9 2,615,848 + MPMX20_02475 0.24 -1.1 2,615,848 + MPMX20_02475 0.24 -1.1 2,615,848 + MPMX20_02475 0.24 -0.9 2,615,856 - MPMX20_02475 0.25 -1.5 2,615,960 + MPMX20_02475 0.35 -0.1 2,615,964 - MPMX20_02475 0.36 +0.9 2,616,022 + MPMX20_02475 0.42 +0.3 2,616,027 + MPMX20_02475 0.42 +0.9 2,616,126 + MPMX20_02475 0.52 -1.4 2,616,172 + MPMX20_02475 0.57 -0.1 2,616,235 + MPMX20_02475 0.64 -1.9 2,616,243 - MPMX20_02475 0.65 -0.1 2,616,243 - MPMX20_02475 0.65 -0.1 2,616,243 - MPMX20_02475 0.65 -1.7 2,616,328 - MPMX20_02475 0.73 -0.1 2,616,412 + MPMX20_02475 0.82 -2.1 2,616,425 - MPMX20_02475 0.83 +0.3 2,616,425 - MPMX20_02475 0.83 -1.1 2,616,542 + +0.6 2,616,585 + -2.1 2,616,721 - MPMX20_02476 0.17 +0.9 2,616,789 + MPMX20_02476 0.24 +0.4 2,616,931 - MPMX20_02476 0.40 -1.1 2,616,981 - MPMX20_02476 0.46 +0.4 2,617,099 + MPMX20_02476 0.59 -0.6 2,617,150 + MPMX20_02476 0.65 -0.9 2,617,158 - MPMX20_02476 0.66 +1.2 2,617,296 + MPMX20_02476 0.81 +0.1 2,617,349 + MPMX20_02476 0.87 -0.6 2,617,426 + +2.9 2,617,426 + -0.9 2,617,426 + +1.4 2,617,426 + -0.9 2,617,434 - -1.1 2,617,434 - -0.6 2,617,540 + -0.9 2,617,546 + -1.7 2,617,548 - -1.1
Or see this region's nucleotide sequence