Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00784 and MPMX20_00785 overlap by 8 nucleotides MPMX20_00785 and MPMX20_00786 are separated by 1 nucleotides MPMX20_00786 and MPMX20_00787 overlap by 4 nucleotides
MPMX20_00784: MPMX20_00784 - UDP-N-acetylmuramate--L-alanine ligase, at 819,841 to 821,316
_00784
MPMX20_00785: MPMX20_00785 - D-alanine--D-alanine ligase B, at 821,309 to 822,229
_00785
MPMX20_00786: MPMX20_00786 - Cell division protein FtsQ, at 822,231 to 823,073
_00786
MPMX20_00787: MPMX20_00787 - Cell division protein FtsA, at 823,070 to 824,326
_00787
Position (kb)
821
822
823 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 821.290 kb on + strand at 821.299 kb on + strand at 821.455 kb on + strand, within MPMX20_00785 at 821.520 kb on + strand, within MPMX20_00785 at 821.594 kb on + strand, within MPMX20_00785 at 821.623 kb on + strand, within MPMX20_00785 at 821.630 kb on + strand, within MPMX20_00785 at 821.709 kb on + strand, within MPMX20_00785 at 821.753 kb on + strand, within MPMX20_00785 at 821.770 kb on + strand, within MPMX20_00785 at 821.770 kb on + strand, within MPMX20_00785 at 822.057 kb on + strand, within MPMX20_00785 at 822.214 kb on + strand at 822.233 kb on + strand at 823.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 821,290 + +0.5 821,299 + -0.0 821,455 + MPMX20_00785 0.16 -2.2 821,520 + MPMX20_00785 0.23 +0.6 821,594 + MPMX20_00785 0.31 -2.2 821,623 + MPMX20_00785 0.34 +0.8 821,630 + MPMX20_00785 0.35 -0.0 821,709 + MPMX20_00785 0.43 -1.1 821,753 + MPMX20_00785 0.48 +0.6 821,770 + MPMX20_00785 0.50 -1.7 821,770 + MPMX20_00785 0.50 -0.7 822,057 + MPMX20_00785 0.81 -0.5 822,214 + +0.3 822,233 + +1.1 823,052 + +2.1
Or see this region's nucleotide sequence