Strain Fitness in Enterobacter sp. TBS_079 around MPMX20_00728

Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMPMX20_00726 and MPMX20_00727 overlap by 1 nucleotidesMPMX20_00727 and MPMX20_00728 are separated by 92 nucleotidesMPMX20_00728 and MPMX20_00729 are separated by 1 nucleotides MPMX20_00726: MPMX20_00726 - Isoleucine--tRNA ligase, at 749,923 to 752,739 _00726 MPMX20_00727: MPMX20_00727 - Lipoprotein signal peptidase, at 752,739 to 753,239 _00727 MPMX20_00728: MPMX20_00728 - FKBP-type 16 kDa peptidyl-prolyl cis-trans isomerase, at 753,332 to 753,781 _00728 MPMX20_00729: MPMX20_00729 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 753,783 to 754,733 _00729 Position (kb) 753 754Strain fitness (log2 ratio) -1 0 1at 753.344 kb on + strandat 753.566 kb on + strand, within MPMX20_00728at 753.701 kb on + strand, within MPMX20_00728at 754.750 kb on + strandat 754.751 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
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753,344 + -1.0
753,566 + MPMX20_00728 0.52 -1.2
753,701 + MPMX20_00728 0.82 -1.3
754,750 + +0.5
754,751 - +0.6

Or see this region's nucleotide sequence