Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00418 and MPMX20_00419 are separated by 116 nucleotides MPMX20_00419 and MPMX20_00420 are separated by 335 nucleotides MPMX20_00420 and MPMX20_00421 are separated by 42 nucleotides
MPMX20_00418: MPMX20_00418 - Anaerobic C4-dicarboxylate transporter DcuA, at 433,804 to 435,105
_00418
MPMX20_00419: MPMX20_00419 - Aspartate ammonia-lyase, at 435,222 to 436,658
_00419
MPMX20_00420: MPMX20_00420 - hypothetical protein, at 436,994 to 437,458
_00420
MPMX20_00421: MPMX20_00421 - L-methionine/branched-chain amino acid exporter YjeH, at 437,501 to 438,739
_00421
Position (kb) 435
436
437 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 434.240 kb on - strand, within MPMX20_00418 at 434.282 kb on + strand, within MPMX20_00418 at 434.290 kb on - strand, within MPMX20_00418 at 434.340 kb on + strand, within MPMX20_00418 at 434.358 kb on + strand, within MPMX20_00418 at 434.366 kb on - strand, within MPMX20_00418 at 434.392 kb on - strand, within MPMX20_00418 at 434.397 kb on - strand, within MPMX20_00418 at 434.427 kb on - strand, within MPMX20_00418 at 434.427 kb on - strand, within MPMX20_00418 at 434.558 kb on - strand, within MPMX20_00418 at 434.573 kb on - strand, within MPMX20_00418 at 434.627 kb on + strand, within MPMX20_00418 at 434.627 kb on + strand, within MPMX20_00418 at 434.631 kb on + strand, within MPMX20_00418 at 434.671 kb on - strand, within MPMX20_00418 at 434.727 kb on + strand, within MPMX20_00418 at 434.729 kb on + strand, within MPMX20_00418 at 434.759 kb on - strand, within MPMX20_00418 at 434.769 kb on - strand, within MPMX20_00418 at 434.773 kb on + strand, within MPMX20_00418 at 434.807 kb on + strand, within MPMX20_00418 at 434.815 kb on - strand, within MPMX20_00418 at 434.911 kb on - strand, within MPMX20_00418 at 434.919 kb on - strand, within MPMX20_00418 at 434.967 kb on + strand, within MPMX20_00418 at 434.978 kb on + strand at 434.978 kb on + strand at 434.978 kb on + strand at 434.986 kb on - strand at 434.986 kb on - strand at 434.997 kb on + strand at 434.997 kb on + strand at 435.005 kb on - strand at 435.012 kb on + strand at 435.020 kb on - strand at 435.044 kb on + strand at 435.046 kb on + strand at 435.054 kb on - strand at 435.103 kb on + strand at 435.103 kb on + strand at 435.103 kb on + strand at 435.103 kb on - strand at 435.103 kb on - strand at 435.112 kb on - strand at 435.196 kb on + strand at 435.206 kb on + strand at 435.211 kb on + strand at 435.211 kb on + strand at 435.214 kb on - strand at 435.214 kb on - strand at 435.214 kb on - strand at 435.219 kb on - strand at 435.315 kb on - strand at 435.327 kb on + strand at 435.388 kb on + strand, within MPMX20_00419 at 435.410 kb on + strand, within MPMX20_00419 at 435.438 kb on + strand, within MPMX20_00419 at 435.444 kb on + strand, within MPMX20_00419 at 435.444 kb on + strand, within MPMX20_00419 at 435.468 kb on + strand, within MPMX20_00419 at 435.503 kb on + strand, within MPMX20_00419 at 435.514 kb on + strand, within MPMX20_00419 at 435.606 kb on + strand, within MPMX20_00419 at 435.611 kb on + strand, within MPMX20_00419 at 435.621 kb on - strand, within MPMX20_00419 at 435.632 kb on + strand, within MPMX20_00419 at 435.632 kb on + strand, within MPMX20_00419 at 435.640 kb on - strand, within MPMX20_00419 at 435.640 kb on - strand, within MPMX20_00419 at 435.663 kb on - strand, within MPMX20_00419 at 435.693 kb on - strand, within MPMX20_00419 at 435.737 kb on - strand, within MPMX20_00419 at 435.754 kb on - strand, within MPMX20_00419 at 435.759 kb on - strand, within MPMX20_00419 at 435.767 kb on + strand, within MPMX20_00419 at 435.822 kb on - strand, within MPMX20_00419 at 435.884 kb on + strand, within MPMX20_00419 at 435.892 kb on - strand, within MPMX20_00419 at 435.892 kb on - strand, within MPMX20_00419 at 435.893 kb on - strand, within MPMX20_00419 at 435.939 kb on + strand, within MPMX20_00419 at 435.947 kb on - strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.989 kb on + strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 435.997 kb on - strand, within MPMX20_00419 at 436.096 kb on - strand, within MPMX20_00419 at 436.127 kb on - strand, within MPMX20_00419 at 436.154 kb on + strand, within MPMX20_00419 at 436.154 kb on + strand, within MPMX20_00419 at 436.162 kb on - strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.185 kb on + strand, within MPMX20_00419 at 436.193 kb on - strand, within MPMX20_00419 at 436.193 kb on - strand, within MPMX20_00419 at 436.209 kb on + strand, within MPMX20_00419 at 436.213 kb on + strand, within MPMX20_00419 at 436.271 kb on + strand, within MPMX20_00419 at 436.272 kb on + strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.306 kb on - strand, within MPMX20_00419 at 436.327 kb on - strand, within MPMX20_00419 at 436.330 kb on - strand, within MPMX20_00419 at 436.375 kb on + strand, within MPMX20_00419 at 436.404 kb on + strand, within MPMX20_00419 at 436.412 kb on - strand, within MPMX20_00419 at 436.588 kb on + strand at 436.588 kb on + strand at 436.596 kb on - strand at 436.637 kb on - strand at 436.656 kb on + strand at 436.664 kb on - strand at 436.690 kb on + strand at 436.698 kb on - strand at 436.715 kb on + strand at 436.745 kb on - strand at 436.777 kb on - strand at 436.779 kb on + strand at 436.779 kb on + strand at 436.793 kb on + strand at 436.793 kb on + strand at 436.793 kb on + strand at 436.816 kb on - strand at 436.853 kb on - strand at 436.913 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.949 kb on + strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.957 kb on - strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.961 kb on + strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.969 kb on - strand at 436.976 kb on + strand at 436.984 kb on + strand at 436.984 kb on + strand at 436.992 kb on - strand at 436.992 kb on - strand at 436.992 kb on - strand at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.064 kb on + strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.072 kb on - strand, within MPMX20_00420 at 437.076 kb on - strand, within MPMX20_00420 at 437.076 kb on - strand, within MPMX20_00420 at 437.268 kb on - strand, within MPMX20_00420 at 437.270 kb on + strand, within MPMX20_00420 at 437.362 kb on - strand, within MPMX20_00420 at 437.374 kb on - strand, within MPMX20_00420 at 437.378 kb on + strand, within MPMX20_00420 at 437.381 kb on - strand, within MPMX20_00420 at 437.415 kb on + strand at 437.418 kb on - strand at 437.418 kb on - strand at 437.423 kb on - strand at 437.423 kb on - strand at 437.466 kb on - strand at 437.476 kb on - strand at 437.494 kb on - strand at 437.506 kb on - strand at 437.607 kb on + strand at 437.637 kb on + strand, within MPMX20_00421 at 437.652 kb on - strand, within MPMX20_00421
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 434,240 - MPMX20_00418 0.33 +0.7 434,282 + MPMX20_00418 0.37 -0.5 434,290 - MPMX20_00418 0.37 -1.7 434,340 + MPMX20_00418 0.41 -0.9 434,358 + MPMX20_00418 0.43 -1.6 434,366 - MPMX20_00418 0.43 -1.9 434,392 - MPMX20_00418 0.45 -0.4 434,397 - MPMX20_00418 0.46 -1.5 434,427 - MPMX20_00418 0.48 -0.2 434,427 - MPMX20_00418 0.48 -0.0 434,558 - MPMX20_00418 0.58 -0.4 434,573 - MPMX20_00418 0.59 -0.4 434,627 + MPMX20_00418 0.63 -1.4 434,627 + MPMX20_00418 0.63 -2.4 434,631 + MPMX20_00418 0.64 +0.7 434,671 - MPMX20_00418 0.67 +0.3 434,727 + MPMX20_00418 0.71 +1.0 434,729 + MPMX20_00418 0.71 +1.8 434,759 - MPMX20_00418 0.73 +0.1 434,769 - MPMX20_00418 0.74 +1.4 434,773 + MPMX20_00418 0.74 -1.2 434,807 + MPMX20_00418 0.77 -1.9 434,815 - MPMX20_00418 0.78 -1.2 434,911 - MPMX20_00418 0.85 +1.4 434,919 - MPMX20_00418 0.86 -1.3 434,967 + MPMX20_00418 0.89 +1.4 434,978 + +0.7 434,978 + -1.7 434,978 + -0.6 434,986 - -0.2 434,986 - -0.4 434,997 + +0.6 434,997 + -0.5 435,005 - +1.7 435,012 + -1.9 435,020 - +0.4 435,044 + -1.0 435,046 + -0.0 435,054 - -1.4 435,103 + -0.4 435,103 + -2.2 435,103 + -1.0 435,103 - +1.4 435,103 - -0.7 435,112 - +0.3 435,196 + -0.3 435,206 + +0.7 435,211 + -2.8 435,211 + -1.0 435,214 - +0.6 435,214 - -0.2 435,214 - -0.6 435,219 - +1.8 435,315 - -0.9 435,327 + -0.9 435,388 + MPMX20_00419 0.12 -2.2 435,410 + MPMX20_00419 0.13 -1.2 435,438 + MPMX20_00419 0.15 +0.4 435,444 + MPMX20_00419 0.15 -2.1 435,444 + MPMX20_00419 0.15 -1.6 435,468 + MPMX20_00419 0.17 -1.6 435,503 + MPMX20_00419 0.20 -0.8 435,514 + MPMX20_00419 0.20 -0.7 435,606 + MPMX20_00419 0.27 -1.9 435,611 + MPMX20_00419 0.27 -3.8 435,621 - MPMX20_00419 0.28 -3.3 435,632 + MPMX20_00419 0.29 +0.1 435,632 + MPMX20_00419 0.29 -1.4 435,640 - MPMX20_00419 0.29 -0.2 435,640 - MPMX20_00419 0.29 -1.7 435,663 - MPMX20_00419 0.31 -1.0 435,693 - MPMX20_00419 0.33 -2.3 435,737 - MPMX20_00419 0.36 +2.4 435,754 - MPMX20_00419 0.37 -0.2 435,759 - MPMX20_00419 0.37 -1.7 435,767 + MPMX20_00419 0.38 -0.9 435,822 - MPMX20_00419 0.42 -2.7 435,884 + MPMX20_00419 0.46 -0.2 435,892 - MPMX20_00419 0.47 +0.0 435,892 - MPMX20_00419 0.47 -1.7 435,893 - MPMX20_00419 0.47 -3.7 435,939 + MPMX20_00419 0.50 -0.9 435,947 - MPMX20_00419 0.50 -0.2 435,989 + MPMX20_00419 0.53 +0.3 435,989 + MPMX20_00419 0.53 -1.7 435,989 + MPMX20_00419 0.53 -0.6 435,997 - MPMX20_00419 0.54 +1.4 435,997 - MPMX20_00419 0.54 -1.0 435,997 - MPMX20_00419 0.54 -0.7 435,997 - MPMX20_00419 0.54 +0.4 435,997 - MPMX20_00419 0.54 -3.2 436,096 - MPMX20_00419 0.61 -0.9 436,127 - MPMX20_00419 0.63 +1.4 436,154 + MPMX20_00419 0.65 -1.8 436,154 + MPMX20_00419 0.65 -2.9 436,162 - MPMX20_00419 0.65 -0.9 436,185 + MPMX20_00419 0.67 -1.6 436,185 + MPMX20_00419 0.67 -1.4 436,185 + MPMX20_00419 0.67 -1.3 436,185 + MPMX20_00419 0.67 -3.9 436,185 + MPMX20_00419 0.67 -1.6 436,185 + MPMX20_00419 0.67 +2.2 436,193 - MPMX20_00419 0.68 -1.7 436,193 - MPMX20_00419 0.68 -2.9 436,209 + MPMX20_00419 0.69 -1.8 436,213 + MPMX20_00419 0.69 -2.2 436,271 + MPMX20_00419 0.73 -2.5 436,272 + MPMX20_00419 0.73 -1.7 436,306 - MPMX20_00419 0.75 -2.3 436,306 - MPMX20_00419 0.75 +2.4 436,306 - MPMX20_00419 0.75 -1.7 436,327 - MPMX20_00419 0.77 -1.9 436,330 - MPMX20_00419 0.77 -2.9 436,375 + MPMX20_00419 0.80 -2.2 436,404 + MPMX20_00419 0.82 +1.4 436,412 - MPMX20_00419 0.83 -0.9 436,588 + -0.7 436,588 + -1.4 436,596 - -3.2 436,637 - -2.0 436,656 + -1.0 436,664 - +0.8 436,690 + -3.8 436,698 - -0.5 436,715 + -1.9 436,745 - -0.0 436,777 - -0.1 436,779 + -0.7 436,779 + +1.1 436,793 + -1.2 436,793 + -2.2 436,793 + +1.8 436,816 - -1.4 436,853 - -0.8 436,913 + +1.8 436,949 + -0.4 436,949 + +0.6 436,949 + -1.6 436,949 + +0.1 436,949 + +1.0 436,949 + -0.3 436,949 + +0.1 436,949 + -0.4 436,949 + -0.6 436,949 + +0.3 436,957 - -1.4 436,957 - -1.1 436,957 - -1.2 436,957 - -3.1 436,957 - -0.2 436,957 - +1.6 436,957 - -1.8 436,957 - -0.2 436,957 - -1.6 436,957 - -0.0 436,957 - -0.6 436,957 - -0.1 436,957 - -1.6 436,957 - -0.9 436,957 - +0.6 436,957 - -1.1 436,957 - -0.0 436,957 - -0.0 436,957 - +0.3 436,961 + -0.4 436,961 + -0.4 436,961 + -3.2 436,961 + +0.8 436,969 - +0.0 436,969 - -0.9 436,969 - +1.0 436,969 - -1.0 436,976 + +0.3 436,984 + -0.7 436,984 + +0.5 436,992 - +0.6 436,992 - -0.1 436,992 - -1.9 437,064 + MPMX20_00420 0.15 -0.3 437,064 + MPMX20_00420 0.15 -1.3 437,064 + MPMX20_00420 0.15 +0.4 437,064 + MPMX20_00420 0.15 -0.3 437,064 + MPMX20_00420 0.15 +1.4 437,064 + MPMX20_00420 0.15 +0.0 437,064 + MPMX20_00420 0.15 -1.2 437,064 + MPMX20_00420 0.15 -0.6 437,064 + MPMX20_00420 0.15 -0.2 437,064 + MPMX20_00420 0.15 -3.0 437,064 + MPMX20_00420 0.15 +0.1 437,064 + MPMX20_00420 0.15 -0.9 437,064 + MPMX20_00420 0.15 -1.3 437,064 + MPMX20_00420 0.15 +0.1 437,064 + MPMX20_00420 0.15 -0.8 437,064 + MPMX20_00420 0.15 -0.3 437,072 - MPMX20_00420 0.17 -0.6 437,072 - MPMX20_00420 0.17 +0.7 437,072 - MPMX20_00420 0.17 -0.3 437,072 - MPMX20_00420 0.17 +0.7 437,072 - MPMX20_00420 0.17 +2.0 437,072 - MPMX20_00420 0.17 -0.6 437,072 - MPMX20_00420 0.17 -1.4 437,072 - MPMX20_00420 0.17 -2.6 437,072 - MPMX20_00420 0.17 -1.9 437,072 - MPMX20_00420 0.17 -0.4 437,072 - MPMX20_00420 0.17 -1.1 437,076 - MPMX20_00420 0.18 -1.2 437,076 - MPMX20_00420 0.18 -0.6 437,268 - MPMX20_00420 0.59 +0.6 437,270 + MPMX20_00420 0.59 -0.3 437,362 - MPMX20_00420 0.79 -1.2 437,374 - MPMX20_00420 0.82 +0.4 437,378 + MPMX20_00420 0.83 -2.4 437,381 - MPMX20_00420 0.83 -0.4 437,415 + +1.4 437,418 - -0.1 437,418 - +0.7 437,423 - +0.3 437,423 - -0.9 437,466 - -0.6 437,476 - -0.2 437,494 - -0.3 437,506 - -0.9 437,607 + -0.2 437,637 + MPMX20_00421 0.11 -1.1 437,652 - MPMX20_00421 0.12 -0.2
Or see this region's nucleotide sequence