Experiment: 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00273 and MPMX20_00274 are separated by 113 nucleotides MPMX20_00274 and MPMX20_00275 are separated by 2 nucleotides MPMX20_00275 and MPMX20_00276 are separated by 3 nucleotides MPMX20_00276 and MPMX20_00277 are separated by 251 nucleotides MPMX20_00277 and MPMX20_00278 are separated by 64 nucleotides
MPMX20_00273: MPMX20_00273 - Class B acid phosphatase, at 290,935 to 291,648
_00273
MPMX20_00274: MPMX20_00274 - hypothetical protein, at 291,762 to 292,178
_00274
MPMX20_00275: MPMX20_00275 - putative protein YjbR, at 292,181 to 292,534
_00275
MPMX20_00276: MPMX20_00276 - UvrABC system protein A, at 292,538 to 295,360
_00276
MPMX20_00277: MPMX20_00277 - Single-stranded DNA-binding protein, at 295,612 to 296,139
_00277
MPMX20_00278: MPMX20_00278 - hypothetical protein, at 296,204 to 296,485
_00278
Position (kb) 292
293
294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 291.554 kb on + strand, within MPMX20_00273 at 291.642 kb on + strand at 291.761 kb on - strand at 291.761 kb on - strand at 291.766 kb on - strand at 291.785 kb on - strand at 291.867 kb on + strand, within MPMX20_00274 at 291.867 kb on + strand, within MPMX20_00274 at 291.869 kb on - strand, within MPMX20_00274 at 291.875 kb on - strand, within MPMX20_00274 at 291.893 kb on + strand, within MPMX20_00274 at 291.895 kb on + strand, within MPMX20_00274 at 291.903 kb on - strand, within MPMX20_00274 at 291.943 kb on - strand, within MPMX20_00274 at 292.019 kb on - strand, within MPMX20_00274 at 292.044 kb on + strand, within MPMX20_00274 at 292.081 kb on - strand, within MPMX20_00274 at 292.091 kb on + strand, within MPMX20_00274 at 292.091 kb on + strand, within MPMX20_00274 at 292.207 kb on - strand at 292.222 kb on - strand, within MPMX20_00275 at 292.229 kb on + strand, within MPMX20_00275 at 292.229 kb on + strand, within MPMX20_00275 at 292.237 kb on - strand, within MPMX20_00275 at 292.264 kb on + strand, within MPMX20_00275 at 292.272 kb on - strand, within MPMX20_00275 at 292.272 kb on - strand, within MPMX20_00275 at 292.272 kb on - strand, within MPMX20_00275 at 292.275 kb on + strand, within MPMX20_00275 at 292.303 kb on + strand, within MPMX20_00275 at 292.304 kb on + strand, within MPMX20_00275 at 292.312 kb on - strand, within MPMX20_00275 at 292.312 kb on - strand, within MPMX20_00275 at 292.322 kb on - strand, within MPMX20_00275 at 292.327 kb on + strand, within MPMX20_00275 at 292.328 kb on + strand, within MPMX20_00275 at 292.338 kb on + strand, within MPMX20_00275 at 292.346 kb on - strand, within MPMX20_00275 at 292.346 kb on - strand, within MPMX20_00275 at 292.354 kb on + strand, within MPMX20_00275 at 292.380 kb on - strand, within MPMX20_00275 at 292.402 kb on - strand, within MPMX20_00275 at 292.409 kb on + strand, within MPMX20_00275 at 292.481 kb on - strand, within MPMX20_00275 at 292.488 kb on + strand, within MPMX20_00275 at 292.527 kb on + strand at 292.568 kb on + strand at 292.575 kb on + strand at 292.576 kb on - strand at 292.583 kb on - strand at 292.592 kb on + strand at 292.647 kb on - strand at 292.671 kb on + strand at 292.710 kb on + strand at 292.718 kb on - strand at 292.777 kb on - strand at 292.777 kb on - strand at 292.821 kb on + strand, within MPMX20_00276 at 292.855 kb on + strand, within MPMX20_00276 at 292.863 kb on + strand, within MPMX20_00276 at 292.871 kb on - strand, within MPMX20_00276 at 292.919 kb on - strand, within MPMX20_00276 at 292.933 kb on + strand, within MPMX20_00276 at 292.940 kb on + strand, within MPMX20_00276 at 292.954 kb on - strand, within MPMX20_00276 at 292.991 kb on + strand, within MPMX20_00276 at 292.991 kb on + strand, within MPMX20_00276 at 292.991 kb on + strand, within MPMX20_00276 at 292.999 kb on - strand, within MPMX20_00276 at 293.009 kb on + strand, within MPMX20_00276 at 293.058 kb on + strand, within MPMX20_00276 at 293.058 kb on + strand, within MPMX20_00276 at 293.066 kb on - strand, within MPMX20_00276 at 293.103 kb on + strand, within MPMX20_00276 at 293.113 kb on + strand, within MPMX20_00276 at 293.121 kb on - strand, within MPMX20_00276 at 293.145 kb on - strand, within MPMX20_00276 at 293.158 kb on + strand, within MPMX20_00276 at 293.179 kb on + strand, within MPMX20_00276 at 293.179 kb on + strand, within MPMX20_00276 at 293.187 kb on - strand, within MPMX20_00276 at 293.187 kb on - strand, within MPMX20_00276 at 293.187 kb on - strand, within MPMX20_00276 at 293.187 kb on - strand, within MPMX20_00276 at 293.211 kb on + strand, within MPMX20_00276 at 293.211 kb on + strand, within MPMX20_00276 at 293.219 kb on + strand, within MPMX20_00276 at 293.219 kb on + strand, within MPMX20_00276 at 293.219 kb on - strand, within MPMX20_00276 at 293.219 kb on - strand, within MPMX20_00276 at 293.219 kb on - strand, within MPMX20_00276 at 293.225 kb on + strand, within MPMX20_00276 at 293.232 kb on + strand, within MPMX20_00276 at 293.232 kb on + strand, within MPMX20_00276 at 293.232 kb on + strand, within MPMX20_00276 at 293.233 kb on - strand, within MPMX20_00276 at 293.253 kb on - strand, within MPMX20_00276 at 293.283 kb on + strand, within MPMX20_00276 at 293.320 kb on + strand, within MPMX20_00276 at 293.320 kb on + strand, within MPMX20_00276 at 293.372 kb on + strand, within MPMX20_00276 at 293.380 kb on - strand, within MPMX20_00276 at 293.391 kb on + strand, within MPMX20_00276 at 293.391 kb on + strand, within MPMX20_00276 at 293.391 kb on + strand, within MPMX20_00276 at 293.399 kb on - strand, within MPMX20_00276 at 293.399 kb on - strand, within MPMX20_00276 at 293.406 kb on + strand, within MPMX20_00276 at 293.409 kb on - strand, within MPMX20_00276 at 293.414 kb on - strand, within MPMX20_00276 at 293.424 kb on - strand, within MPMX20_00276 at 293.487 kb on - strand, within MPMX20_00276 at 293.563 kb on + strand, within MPMX20_00276 at 293.583 kb on + strand, within MPMX20_00276 at 293.591 kb on - strand, within MPMX20_00276 at 293.606 kb on + strand, within MPMX20_00276 at 293.614 kb on - strand, within MPMX20_00276 at 293.614 kb on - strand, within MPMX20_00276 at 293.660 kb on + strand, within MPMX20_00276 at 293.738 kb on - strand, within MPMX20_00276 at 293.785 kb on - strand, within MPMX20_00276 at 293.800 kb on + strand, within MPMX20_00276 at 293.843 kb on + strand, within MPMX20_00276 at 293.843 kb on + strand, within MPMX20_00276 at 293.851 kb on - strand, within MPMX20_00276 at 293.851 kb on - strand, within MPMX20_00276 at 293.851 kb on - strand, within MPMX20_00276 at 293.856 kb on - strand, within MPMX20_00276 at 293.858 kb on + strand, within MPMX20_00276 at 293.879 kb on + strand, within MPMX20_00276 at 293.957 kb on + strand, within MPMX20_00276 at 293.970 kb on - strand, within MPMX20_00276 at 294.019 kb on + strand, within MPMX20_00276 at 294.019 kb on + strand, within MPMX20_00276 at 294.027 kb on - strand, within MPMX20_00276 at 294.061 kb on + strand, within MPMX20_00276 at 294.061 kb on + strand, within MPMX20_00276 at 294.069 kb on - strand, within MPMX20_00276 at 294.124 kb on - strand, within MPMX20_00276 at 294.124 kb on - strand, within MPMX20_00276 at 294.138 kb on - strand, within MPMX20_00276 at 294.176 kb on + strand, within MPMX20_00276 at 294.222 kb on - strand, within MPMX20_00276 at 294.222 kb on - strand, within MPMX20_00276 at 294.233 kb on - strand, within MPMX20_00276 at 294.317 kb on - strand, within MPMX20_00276 at 294.322 kb on - strand, within MPMX20_00276 at 294.332 kb on - strand, within MPMX20_00276 at 294.411 kb on + strand, within MPMX20_00276 at 294.463 kb on + strand, within MPMX20_00276 at 294.471 kb on - strand, within MPMX20_00276 at 294.514 kb on + strand, within MPMX20_00276 at 294.522 kb on - strand, within MPMX20_00276 at 294.568 kb on + strand, within MPMX20_00276 at 294.568 kb on + strand, within MPMX20_00276 at 294.568 kb on + strand, within MPMX20_00276 at 294.618 kb on + strand, within MPMX20_00276 at 294.781 kb on + strand, within MPMX20_00276 at 294.781 kb on + strand, within MPMX20_00276 at 294.786 kb on + strand, within MPMX20_00276 at 294.786 kb on + strand, within MPMX20_00276 at 294.798 kb on + strand, within MPMX20_00276 at 294.842 kb on + strand, within MPMX20_00276 at 294.882 kb on + strand, within MPMX20_00276 at 294.890 kb on - strand, within MPMX20_00276 at 294.890 kb on - strand, within MPMX20_00276 at 294.890 kb on - strand, within MPMX20_00276 at 294.890 kb on - strand, within MPMX20_00276 at 294.892 kb on + strand, within MPMX20_00276 at 294.892 kb on + strand, within MPMX20_00276 at 294.900 kb on - strand, within MPMX20_00276 at 294.961 kb on + strand, within MPMX20_00276 at 295.036 kb on - strand, within MPMX20_00276 at 295.043 kb on + strand, within MPMX20_00276 at 295.119 kb on + strand at 295.127 kb on + strand at 295.127 kb on + strand at 295.135 kb on - strand at 295.169 kb on + strand at 295.169 kb on + strand at 295.188 kb on + strand at 295.190 kb on + strand at 295.232 kb on + strand at 295.365 kb on - strand at 295.365 kb on - strand at 295.482 kb on + strand at 295.490 kb on + strand at 295.498 kb on - strand at 295.505 kb on - strand at 295.525 kb on - strand at 296.149 kb on + strand at 296.200 kb on + strand at 296.223 kb on - strand at 296.233 kb on + strand, within MPMX20_00278 at 296.233 kb on + strand, within MPMX20_00278 at 296.241 kb on - strand, within MPMX20_00278 at 296.241 kb on - strand, within MPMX20_00278 at 296.241 kb on - strand, within MPMX20_00278 at 296.247 kb on - strand, within MPMX20_00278 at 296.248 kb on - strand, within MPMX20_00278 at 296.271 kb on - strand, within MPMX20_00278 at 296.304 kb on + strand, within MPMX20_00278 at 296.307 kb on - strand, within MPMX20_00278 at 296.317 kb on - strand, within MPMX20_00278 at 296.322 kb on - strand, within MPMX20_00278 at 296.343 kb on + strand, within MPMX20_00278 at 296.345 kb on + strand, within MPMX20_00278 at 296.345 kb on + strand, within MPMX20_00278 at 296.355 kb on + strand, within MPMX20_00278
Per-strain Table
Position Strand Gene LocusTag Fraction 0.1X LB plate, Mixed culture; Streptomyces sp. SAI_126 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct remove 291,554 + MPMX20_00273 0.87 +0.2 291,642 + +1.0 291,761 - -0.9 291,761 - +0.3 291,766 - +0.6 291,785 - -0.0 291,867 + MPMX20_00274 0.25 +1.1 291,867 + MPMX20_00274 0.25 -0.1 291,869 - MPMX20_00274 0.26 -0.2 291,875 - MPMX20_00274 0.27 -1.7 291,893 + MPMX20_00274 0.31 +0.4 291,895 + MPMX20_00274 0.32 +1.4 291,903 - MPMX20_00274 0.34 -0.0 291,943 - MPMX20_00274 0.43 -0.7 292,019 - MPMX20_00274 0.62 -1.9 292,044 + MPMX20_00274 0.68 -0.1 292,081 - MPMX20_00274 0.76 -0.4 292,091 + MPMX20_00274 0.79 +0.2 292,091 + MPMX20_00274 0.79 -0.6 292,207 - -0.6 292,222 - MPMX20_00275 0.12 -0.4 292,229 + MPMX20_00275 0.14 +0.7 292,229 + MPMX20_00275 0.14 +0.8 292,237 - MPMX20_00275 0.16 -0.6 292,264 + MPMX20_00275 0.23 -1.3 292,272 - MPMX20_00275 0.26 -0.2 292,272 - MPMX20_00275 0.26 +0.1 292,272 - MPMX20_00275 0.26 -0.3 292,275 + MPMX20_00275 0.27 +0.8 292,303 + MPMX20_00275 0.34 +0.5 292,304 + MPMX20_00275 0.35 +0.2 292,312 - MPMX20_00275 0.37 +1.0 292,312 - MPMX20_00275 0.37 +2.4 292,322 - MPMX20_00275 0.40 -0.2 292,327 + MPMX20_00275 0.41 -0.3 292,328 + MPMX20_00275 0.42 -0.6 292,338 + MPMX20_00275 0.44 -1.8 292,346 - MPMX20_00275 0.47 -1.1 292,346 - MPMX20_00275 0.47 -1.2 292,354 + MPMX20_00275 0.49 +1.4 292,380 - MPMX20_00275 0.56 -0.0 292,402 - MPMX20_00275 0.62 -0.5 292,409 + MPMX20_00275 0.64 -0.6 292,481 - MPMX20_00275 0.85 -0.2 292,488 + MPMX20_00275 0.87 -1.0 292,527 + +2.4 292,568 + -1.4 292,575 + -1.3 292,576 - -1.8 292,583 - -0.5 292,592 + -2.9 292,647 - -0.6 292,671 + -0.5 292,710 + -1.0 292,718 - -3.2 292,777 - -0.2 292,777 - -2.3 292,821 + MPMX20_00276 0.10 -0.9 292,855 + MPMX20_00276 0.11 -2.4 292,863 + MPMX20_00276 0.12 +0.8 292,871 - MPMX20_00276 0.12 -0.9 292,919 - MPMX20_00276 0.13 -3.4 292,933 + MPMX20_00276 0.14 -2.1 292,940 + MPMX20_00276 0.14 -0.2 292,954 - MPMX20_00276 0.15 -1.7 292,991 + MPMX20_00276 0.16 -2.3 292,991 + MPMX20_00276 0.16 -3.1 292,991 + MPMX20_00276 0.16 +0.1 292,999 - MPMX20_00276 0.16 -2.4 293,009 + MPMX20_00276 0.17 -0.9 293,058 + MPMX20_00276 0.18 -1.9 293,058 + MPMX20_00276 0.18 -0.8 293,066 - MPMX20_00276 0.19 -0.9 293,103 + MPMX20_00276 0.20 -0.7 293,113 + MPMX20_00276 0.20 -0.9 293,121 - MPMX20_00276 0.21 -0.2 293,145 - MPMX20_00276 0.22 +0.5 293,158 + MPMX20_00276 0.22 +0.6 293,179 + MPMX20_00276 0.23 -1.6 293,179 + MPMX20_00276 0.23 -1.7 293,187 - MPMX20_00276 0.23 -0.5 293,187 - MPMX20_00276 0.23 -0.6 293,187 - MPMX20_00276 0.23 -1.6 293,187 - MPMX20_00276 0.23 -3.0 293,211 + MPMX20_00276 0.24 -0.7 293,211 + MPMX20_00276 0.24 -1.0 293,219 + MPMX20_00276 0.24 -0.6 293,219 + MPMX20_00276 0.24 -0.9 293,219 - MPMX20_00276 0.24 -1.2 293,219 - MPMX20_00276 0.24 +0.4 293,219 - MPMX20_00276 0.24 -1.2 293,225 + MPMX20_00276 0.24 -0.9 293,232 + MPMX20_00276 0.25 -1.2 293,232 + MPMX20_00276 0.25 -1.0 293,232 + MPMX20_00276 0.25 -2.2 293,233 - MPMX20_00276 0.25 -1.5 293,253 - MPMX20_00276 0.25 +0.1 293,283 + MPMX20_00276 0.26 +0.8 293,320 + MPMX20_00276 0.28 -1.4 293,320 + MPMX20_00276 0.28 -1.0 293,372 + MPMX20_00276 0.30 +0.4 293,380 - MPMX20_00276 0.30 -1.6 293,391 + MPMX20_00276 0.30 -0.3 293,391 + MPMX20_00276 0.30 -3.1 293,391 + MPMX20_00276 0.30 -1.2 293,399 - MPMX20_00276 0.30 -0.1 293,399 - MPMX20_00276 0.30 -1.9 293,406 + MPMX20_00276 0.31 -1.6 293,409 - MPMX20_00276 0.31 -0.6 293,414 - MPMX20_00276 0.31 -3.2 293,424 - MPMX20_00276 0.31 -0.7 293,487 - MPMX20_00276 0.34 -0.2 293,563 + MPMX20_00276 0.36 -1.5 293,583 + MPMX20_00276 0.37 -2.8 293,591 - MPMX20_00276 0.37 -1.0 293,606 + MPMX20_00276 0.38 -0.2 293,614 - MPMX20_00276 0.38 -0.3 293,614 - MPMX20_00276 0.38 -0.4 293,660 + MPMX20_00276 0.40 -2.0 293,738 - MPMX20_00276 0.43 -0.6 293,785 - MPMX20_00276 0.44 -2.3 293,800 + MPMX20_00276 0.45 -0.7 293,843 + MPMX20_00276 0.46 -0.6 293,843 + MPMX20_00276 0.46 +0.4 293,851 - MPMX20_00276 0.47 +0.2 293,851 - MPMX20_00276 0.47 -1.3 293,851 - MPMX20_00276 0.47 -0.8 293,856 - MPMX20_00276 0.47 -2.0 293,858 + MPMX20_00276 0.47 -1.1 293,879 + MPMX20_00276 0.48 +0.0 293,957 + MPMX20_00276 0.50 -1.4 293,970 - MPMX20_00276 0.51 -1.3 294,019 + MPMX20_00276 0.52 -1.6 294,019 + MPMX20_00276 0.52 +0.8 294,027 - MPMX20_00276 0.53 -1.2 294,061 + MPMX20_00276 0.54 -0.7 294,061 + MPMX20_00276 0.54 -2.0 294,069 - MPMX20_00276 0.54 -2.1 294,124 - MPMX20_00276 0.56 -0.8 294,124 - MPMX20_00276 0.56 -0.1 294,138 - MPMX20_00276 0.57 +2.4 294,176 + MPMX20_00276 0.58 -1.7 294,222 - MPMX20_00276 0.60 -0.9 294,222 - MPMX20_00276 0.60 -0.9 294,233 - MPMX20_00276 0.60 -0.2 294,317 - MPMX20_00276 0.63 -1.6 294,322 - MPMX20_00276 0.63 -2.2 294,332 - MPMX20_00276 0.64 -0.8 294,411 + MPMX20_00276 0.66 -2.1 294,463 + MPMX20_00276 0.68 -1.3 294,471 - MPMX20_00276 0.68 -1.4 294,514 + MPMX20_00276 0.70 +0.4 294,522 - MPMX20_00276 0.70 +0.5 294,568 + MPMX20_00276 0.72 -2.1 294,568 + MPMX20_00276 0.72 -0.2 294,568 + MPMX20_00276 0.72 +1.4 294,618 + MPMX20_00276 0.74 -1.5 294,781 + MPMX20_00276 0.79 +0.4 294,781 + MPMX20_00276 0.79 -1.9 294,786 + MPMX20_00276 0.80 -3.2 294,786 + MPMX20_00276 0.80 +1.0 294,798 + MPMX20_00276 0.80 -0.5 294,842 + MPMX20_00276 0.82 -1.2 294,882 + MPMX20_00276 0.83 -0.5 294,890 - MPMX20_00276 0.83 -2.3 294,890 - MPMX20_00276 0.83 -2.5 294,890 - MPMX20_00276 0.83 -1.0 294,890 - MPMX20_00276 0.83 -1.9 294,892 + MPMX20_00276 0.83 +0.2 294,892 + MPMX20_00276 0.83 -2.1 294,900 - MPMX20_00276 0.84 -0.0 294,961 + MPMX20_00276 0.86 +1.4 295,036 - MPMX20_00276 0.88 -1.3 295,043 + MPMX20_00276 0.89 -2.2 295,119 + +0.4 295,127 + +0.4 295,127 + -0.7 295,135 - -0.6 295,169 + +0.4 295,169 + -1.4 295,188 + -0.2 295,190 + -0.6 295,232 + -1.0 295,365 - -0.2 295,365 - -0.2 295,482 + -2.2 295,490 + +1.7 295,498 - -1.6 295,505 - +0.8 295,525 - -1.1 296,149 + -1.7 296,200 + -0.3 296,223 - -0.1 296,233 + MPMX20_00278 0.10 +0.0 296,233 + MPMX20_00278 0.10 -1.9 296,241 - MPMX20_00278 0.13 -0.7 296,241 - MPMX20_00278 0.13 +0.5 296,241 - MPMX20_00278 0.13 +0.2 296,247 - MPMX20_00278 0.15 -0.6 296,248 - MPMX20_00278 0.16 +0.2 296,271 - MPMX20_00278 0.24 -2.4 296,304 + MPMX20_00278 0.35 +0.3 296,307 - MPMX20_00278 0.37 -0.4 296,317 - MPMX20_00278 0.40 -0.4 296,322 - MPMX20_00278 0.42 +0.2 296,343 + MPMX20_00278 0.49 -0.4 296,345 + MPMX20_00278 0.50 -0.6 296,345 + MPMX20_00278 0.50 -1.1 296,355 + MPMX20_00278 0.54 -0.2
Or see this region's nucleotide sequence