Experiment: LB, pH5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13305 and BWI76_RS13310 are separated by 270 nucleotides BWI76_RS13310 and BWI76_RS13315 are separated by 75 nucleotides BWI76_RS13315 and BWI76_RS13320 are separated by 95 nucleotides
BWI76_RS13305: BWI76_RS13305 - hydrolase, at 2,774,665 to 2,775,288
_RS13305
BWI76_RS13310: BWI76_RS13310 - glucan biosynthesis protein D, at 2,775,559 to 2,777,214
_RS13310
BWI76_RS13315: BWI76_RS13315 - 50S ribosomal protein L7/L12-serine acetyltransferase, at 2,777,290 to 2,777,874
_RS13315
BWI76_RS13320: BWI76_RS13320 - putative LpxA-like enzyme, at 2,777,970 to 2,778,950
_RS13320
Position (kb)
2775
2776
2777
2778 Strain fitness (log2 ratio)
-1
0
1 at 2775.271 kb on - strand at 2775.312 kb on - strand at 2775.448 kb on - strand at 2775.795 kb on + strand, within BWI76_RS13310 at 2775.967 kb on - strand, within BWI76_RS13310 at 2776.020 kb on - strand, within BWI76_RS13310 at 2776.116 kb on + strand, within BWI76_RS13310 at 2776.276 kb on - strand, within BWI76_RS13310 at 2776.573 kb on - strand, within BWI76_RS13310 at 2777.103 kb on - strand at 2777.126 kb on - strand at 2777.128 kb on - strand at 2777.367 kb on - strand, within BWI76_RS13315
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH5 remove 2,775,271 - +0.2 2,775,312 - +1.1 2,775,448 - +0.6 2,775,795 + BWI76_RS13310 0.14 +0.2 2,775,967 - BWI76_RS13310 0.25 +0.6 2,776,020 - BWI76_RS13310 0.28 +0.4 2,776,116 + BWI76_RS13310 0.34 +0.8 2,776,276 - BWI76_RS13310 0.43 -0.2 2,776,573 - BWI76_RS13310 0.61 +0.5 2,777,103 - +0.1 2,777,126 - -0.2 2,777,128 - +0.7 2,777,367 - BWI76_RS13315 0.13 +0.0
Or see this region's nucleotide sequence