Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18880

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18870 and BWI76_RS18875 overlap by 4 nucleotidesBWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotidesBWI76_RS18880 and BWI76_RS18885 are separated by 321 nucleotides BWI76_RS18870: BWI76_RS18870 - protein NifZ, at 3,913,502 to 3,913,948 _RS18870 BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745 _RS18875 BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299 _RS18880 BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108 _RS18885 Position (kb) 3914 3915 3916Strain fitness (log2 ratio) -1 0 1 2at 3913.774 kb on - strand, within BWI76_RS18870at 3913.910 kb on + strandat 3914.056 kb on + strand, within BWI76_RS18875at 3914.312 kb on - strand, within BWI76_RS18875at 3914.368 kb on + strand, within BWI76_RS18875at 3914.464 kb on - strand, within BWI76_RS18875at 3914.498 kb on - strand, within BWI76_RS18875at 3914.598 kb on - strand, within BWI76_RS18875at 3914.792 kb on + strandat 3914.813 kb on - strandat 3915.397 kb on - strandat 3915.641 kb on + strandat 3916.112 kb on + strand, within BWI76_RS18885

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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3,913,774 - BWI76_RS18870 0.61 -0.4
3,913,910 + -0.0
3,914,056 + BWI76_RS18875 0.14 +0.5
3,914,312 - BWI76_RS18875 0.46 +1.8
3,914,368 + BWI76_RS18875 0.53 +0.1
3,914,464 - BWI76_RS18875 0.65 -0.2
3,914,498 - BWI76_RS18875 0.69 -0.4
3,914,598 - BWI76_RS18875 0.82 -0.4
3,914,792 + +0.4
3,914,813 - -1.5
3,915,397 - -0.8
3,915,641 + -0.2
3,916,112 + BWI76_RS18885 0.33 -0.5

Or see this region's nucleotide sequence