Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18870 and BWI76_RS18875 overlap by 4 nucleotides BWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotides BWI76_RS18880 and BWI76_RS18885 are separated by 321 nucleotides
BWI76_RS18870: BWI76_RS18870 - protein NifZ, at 3,913,502 to 3,913,948
_RS18870
BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745
_RS18875
BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299
_RS18880
BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108
_RS18885
Position (kb)
3914
3915
3916 Strain fitness (log2 ratio)
-1
0
1
2 at 3913.774 kb on - strand, within BWI76_RS18870 at 3913.910 kb on + strand at 3914.056 kb on + strand, within BWI76_RS18875 at 3914.312 kb on - strand, within BWI76_RS18875 at 3914.368 kb on + strand, within BWI76_RS18875 at 3914.464 kb on - strand, within BWI76_RS18875 at 3914.498 kb on - strand, within BWI76_RS18875 at 3914.598 kb on - strand, within BWI76_RS18875 at 3914.792 kb on + strand at 3914.813 kb on - strand at 3915.397 kb on - strand at 3915.641 kb on + strand at 3916.112 kb on + strand, within BWI76_RS18885
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,913,774 - BWI76_RS18870 0.61 -0.4 3,913,910 + -0.0 3,914,056 + BWI76_RS18875 0.14 +0.5 3,914,312 - BWI76_RS18875 0.46 +1.8 3,914,368 + BWI76_RS18875 0.53 +0.1 3,914,464 - BWI76_RS18875 0.65 -0.2 3,914,498 - BWI76_RS18875 0.69 -0.4 3,914,598 - BWI76_RS18875 0.82 -0.4 3,914,792 + +0.4 3,914,813 - -1.5 3,915,397 - -0.8 3,915,641 + -0.2 3,916,112 + BWI76_RS18885 0.33 -0.5
Or see this region's nucleotide sequence