Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08260 and BWI76_RS08265 are separated by 129 nucleotides BWI76_RS08265 and BWI76_RS08270 are separated by 291 nucleotides BWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotides BWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides
BWI76_RS08260: BWI76_RS08260 - LysR family transcriptional regulator, at 1,749,079 to 1,750,005
_RS08260
BWI76_RS08265: BWI76_RS08265 - transcriptional repressor, at 1,750,135 to 1,750,587
_RS08265
BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508
_RS08270
BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852
_RS08275
BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759
_RS08280
Position (kb) 1750
1751
1752 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1750.030 kb on - strand at 1750.044 kb on + strand at 1750.688 kb on + strand at 1750.810 kb on + strand at 1751.518 kb on + strand at 1751.526 kb on - strand at 1751.571 kb on - strand at 1751.722 kb on + strand, within BWI76_RS08275 at 1751.754 kb on - strand, within BWI76_RS08275 at 1752.008 kb on - strand at 1752.163 kb on - strand, within BWI76_RS08280
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,750,030 - +2.0 1,750,044 + +0.2 1,750,688 + +0.4 1,750,810 + +0.2 1,751,518 + -0.4 1,751,526 - +0.3 1,751,571 - -1.1 1,751,722 + BWI76_RS08275 0.55 -1.8 1,751,754 - BWI76_RS08275 0.66 -2.5 1,752,008 - +0.4 1,752,163 - BWI76_RS08280 0.23 +2.0
Or see this region's nucleotide sequence