Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01295 and BWI76_RS01300 are separated by 138 nucleotides BWI76_RS01300 and BWI76_RS01305 are separated by 70 nucleotides BWI76_RS01305 and BWI76_RS01310 are separated by 13 nucleotides
BWI76_RS01295: BWI76_RS01295 - Uridine phosphorylase (EC 2.4.2.3) <small>(from data)</small>, at 266,181 to 266,942
_RS01295
BWI76_RS01300: BWI76_RS01300 - DNA recombination protein RmuC, at 267,081 to 268,529
_RS01300
BWI76_RS01305: BWI76_RS01305 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 268,600 to 269,355
_RS01305
BWI76_RS01310: BWI76_RS01310 - SCP2 domain-containing protein, at 269,369 to 269,974
_RS01310
Position (kb) 267
268
269 Strain fitness (log2 ratio) -2
-1
0
1
2 at 266.233 kb on + strand at 266.251 kb on + strand at 266.354 kb on - strand, within BWI76_RS01295 at 266.362 kb on - strand at 266.423 kb on + strand, within BWI76_RS01295 at 266.533 kb on + strand, within BWI76_RS01295 at 266.600 kb on + strand, within BWI76_RS01295 at 266.600 kb on + strand, within BWI76_RS01295 at 266.670 kb on - strand, within BWI76_RS01295 at 266.678 kb on + strand, within BWI76_RS01295 at 266.945 kb on - strand at 267.210 kb on + strand at 267.264 kb on - strand, within BWI76_RS01300 at 267.264 kb on - strand, within BWI76_RS01300 at 267.275 kb on + strand, within BWI76_RS01300 at 267.527 kb on + strand, within BWI76_RS01300 at 267.535 kb on - strand, within BWI76_RS01300 at 267.738 kb on - strand, within BWI76_RS01300 at 267.764 kb on - strand, within BWI76_RS01300 at 267.921 kb on + strand, within BWI76_RS01300 at 268.163 kb on - strand, within BWI76_RS01300
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 266,233 + -0.8 266,251 + +0.3 266,354 - BWI76_RS01295 0.23 +0.3 266,362 - +2.6 266,423 + BWI76_RS01295 0.32 +0.3 266,533 + BWI76_RS01295 0.46 -0.2 266,600 + BWI76_RS01295 0.55 +0.9 266,600 + BWI76_RS01295 0.55 +0.7 266,670 - BWI76_RS01295 0.64 +0.4 266,678 + BWI76_RS01295 0.65 +1.0 266,945 - -0.1 267,210 + -1.9 267,264 - BWI76_RS01300 0.13 -1.4 267,264 - BWI76_RS01300 0.13 +0.4 267,275 + BWI76_RS01300 0.13 -0.1 267,527 + BWI76_RS01300 0.31 +0.8 267,535 - BWI76_RS01300 0.31 -0.5 267,738 - BWI76_RS01300 0.45 +0.2 267,764 - BWI76_RS01300 0.47 +2.3 267,921 + BWI76_RS01300 0.58 +1.6 268,163 - BWI76_RS01300 0.75 +0.3
Or see this region's nucleotide sequence