Experiment: KB with Helenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0209 and Psyr_0210 are separated by 42 nucleotides Psyr_0210 and Psyr_0211 are separated by 147 nucleotides Psyr_0211 and Psyr_0212 are separated by 13 nucleotides
Psyr_0209: Psyr_0209 - RelA/SpoT protein, at 223,004 to 225,127
_0209
Psyr_0210: Psyr_0210 - DNA-directed RNA polymerase subunit omega, at 225,170 to 225,433
_0210
Psyr_0211: Psyr_0211 - guanylate kinase, at 225,581 to 226,225
_0211
Psyr_0212: Psyr_0212 - Conserved hypothetical protein 255, at 226,239 to 227,102
_0212
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.151 kb on - strand at 225.166 kb on - strand at 225.166 kb on - strand at 225.253 kb on - strand, within Psyr_0210 at 225.405 kb on - strand, within Psyr_0210 at 225.438 kb on - strand at 225.497 kb on - strand at 225.505 kb on - strand at 225.505 kb on - strand at 225.613 kb on - strand at 225.613 kb on - strand at 226.235 kb on - strand at 226.241 kb on - strand at 226.248 kb on - strand at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 226.341 kb on - strand, within Psyr_0212 at 227.071 kb on - strand at 227.110 kb on - strand at 227.110 kb on - strand at 227.196 kb on - strand at 227.212 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Helenine 10 mM remove 225,151 - +3.8 225,151 - +0.5 225,151 - +0.1 225,151 - -1.2 225,151 - +3.2 225,151 - -1.6 225,166 - +0.2 225,166 - -1.2 225,253 - Psyr_0210 0.31 -2.1 225,405 - Psyr_0210 0.89 +0.2 225,438 - -0.6 225,497 - +0.5 225,505 - -1.3 225,505 - +1.0 225,613 - -0.2 225,613 - -1.9 226,235 - -0.1 226,241 - -0.6 226,248 - -1.1 226,341 - Psyr_0212 0.12 -3.0 226,341 - Psyr_0212 0.12 -1.3 226,341 - Psyr_0212 0.12 -0.8 226,341 - Psyr_0212 0.12 +0.2 226,341 - Psyr_0212 0.12 +2.0 227,071 - -0.4 227,110 - +0.3 227,110 - +0.6 227,196 - +0.2 227,212 - -0.4
Or see this region's nucleotide sequence